TY - JOUR
T1 - Phage therapy against Vibrio parahaemolyticus infection in the whiteleg shrimp (Litopenaeus vannamei) larvae
AU - Lomelí-Ortega, Carlos O.
AU - Martínez-Díaz, Sergio F.
N1 - Funding Information:
This research was supported by the Instituto Politécnico Nacional (project SIP-20100865 ) and by the National Council of Science and Technology CONACyT (Project 85033 and Grant 37176 ).
PY - 2014/10/20
Y1 - 2014/10/20
N2 - Vibrio parahaemolyticus is an important cause of disease, mortality, and economical losses in the shrimp aquaculture industry. Bacteriophages are natural bio-controlling agents, broadly recognized for their ability to reduce pathogen populations. Hence, in the present study, we evaluated the effectiveness of phage therapy in the prevention and control of vibriosis in Litopenaeus vannamei. Vibriosis was induced in shrimp larvae with 2×106CFUmL-1 of V. parahaemolyticus. The infected larvae were treated with different doses of selected phages and their efficacy was evaluated at different times after their application. Results revealed that selected lytic phages (A3S and Vpms1) are effective to reduce mortality caused by V. parahaemolyticus. In both cases, the early application (at 6h post-infection) was effective to avoid mortality. Low multiplicity of infection (MOI) values (<0.1) were enough to counteract V. parahaemolyticus infection. Delayed phage applications (>6h post-infection) hindered mortality and the progress of infection. This study provides the basis for the use of bacteriophages in the prevention and control of V. parahaemolyticus in shrimps.
AB - Vibrio parahaemolyticus is an important cause of disease, mortality, and economical losses in the shrimp aquaculture industry. Bacteriophages are natural bio-controlling agents, broadly recognized for their ability to reduce pathogen populations. Hence, in the present study, we evaluated the effectiveness of phage therapy in the prevention and control of vibriosis in Litopenaeus vannamei. Vibriosis was induced in shrimp larvae with 2×106CFUmL-1 of V. parahaemolyticus. The infected larvae were treated with different doses of selected phages and their efficacy was evaluated at different times after their application. Results revealed that selected lytic phages (A3S and Vpms1) are effective to reduce mortality caused by V. parahaemolyticus. In both cases, the early application (at 6h post-infection) was effective to avoid mortality. Low multiplicity of infection (MOI) values (<0.1) were enough to counteract V. parahaemolyticus infection. Delayed phage applications (>6h post-infection) hindered mortality and the progress of infection. This study provides the basis for the use of bacteriophages in the prevention and control of V. parahaemolyticus in shrimps.
KW - Litopenaeus vannamei
KW - Phage therapy
KW - Shrimp larvae
KW - Vibrio parahaemolyticus
UR - http://www.scopus.com/inward/record.url?scp=84906773648&partnerID=8YFLogxK
U2 - 10.1016/j.aquaculture.2014.08.018
DO - 10.1016/j.aquaculture.2014.08.018
M3 - Artículo
SN - 0044-8486
VL - 434
SP - 208
EP - 211
JO - Aquaculture
JF - Aquaculture
ER -