Perezone and its isomer isoperezone induce caspase-dependent and caspase-independent cell death

L. E. Sánchez-Torres; J. A. Torres-Martínez; M. Godínez-Victoria; J. M. Omar; B. Velasco-Bejarano

doi:10.1016/j.phymed.2009.12.011

Perezone and its isomer isoperezone induce caspase-dependent and caspase-independent cell death

L. E. Sánchez-Torres, J. A. Torres-Martínez, M. Godínez-Victoria, J. M. Omar, B. Velasco-Bejarano

Research output: Contribution to journal › Article › peer-review

14 Scopus citations

Abstract

In this study, we investigated the cytotoxic effect of perezone, a constituent isolated from the roots of Perezia spp. and of its synthetic isomer isoperezone on the K562 human leukemia cell line. Perezone showed greater cytotoxic effect than isoperezone but both compounds were found to induce cytotoxicity trough a caspase-dependent and a caspase-independent mechanisms; important changes in their light scattering properties, phosphatidylserine translocation and mitochondrial membrane potential disruption were detected by cytometry. The mechanism of death induction of each compound showed interesting concentration-dependent differences. Neither compound induced the apoptosis inducing factor.

Original language	English
Pages (from-to)	614-620
Number of pages	7
Journal	Phytomedicine
Volume	17
Issue number	8-9
DOIs	https://doi.org/10.1016/j.phymed.2009.12.011
State	Published - Jul 2010

Keywords

Cell death
Cytotoxic
Isoperezone
Perezone
Sesquiterpenes

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10.1016/j.phymed.2009.12.011

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title = "Perezone and its isomer isoperezone induce caspase-dependent and caspase-independent cell death",

abstract = "In this study, we investigated the cytotoxic effect of perezone, a constituent isolated from the roots of Perezia spp. and of its synthetic isomer isoperezone on the K562 human leukemia cell line. Perezone showed greater cytotoxic effect than isoperezone but both compounds were found to induce cytotoxicity trough a caspase-dependent and a caspase-independent mechanisms; important changes in their light scattering properties, phosphatidylserine translocation and mitochondrial membrane potential disruption were detected by cytometry. The mechanism of death induction of each compound showed interesting concentration-dependent differences. Neither compound induced the apoptosis inducing factor.",

keywords = "Cell death, Cytotoxic, Isoperezone, Perezone, Sesquiterpenes",

author = "S{\'a}nchez-Torres, {L. E.} and Torres-Mart{\'i}nez, {J. A.} and M. God{\'i}nez-Victoria and Omar, {J. M.} and B. Velasco-Bejarano",

note = "Funding Information: This research was financially supported by SIP-IPN . God{\'i}nez-Victoria thanks CONACyT for the scholarship. Luvia Enid S{\'a}nchez-Torres, is a fellow of COFAA-IPN and EDI-IPN. Benjam{\'i}n Velasco Bejarano is a fellow of CONACYT-SNI-08/89717. We thank Alan Larsen for reviewing the use of English in this manuscript.",

year = "2010",

month = jul,

doi = "10.1016/j.phymed.2009.12.011",

language = "Ingl{\'e}s",

volume = "17",

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journal = "Phytomedicine",

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TY - JOUR

T1 - Perezone and its isomer isoperezone induce caspase-dependent and caspase-independent cell death

AU - Sánchez-Torres, L. E.

AU - Torres-Martínez, J. A.

AU - Godínez-Victoria, M.

AU - Omar, J. M.

AU - Velasco-Bejarano, B.

N1 - Funding Information: This research was financially supported by SIP-IPN . Godínez-Victoria thanks CONACyT for the scholarship. Luvia Enid Sánchez-Torres, is a fellow of COFAA-IPN and EDI-IPN. Benjamín Velasco Bejarano is a fellow of CONACYT-SNI-08/89717. We thank Alan Larsen for reviewing the use of English in this manuscript.

PY - 2010/7

Y1 - 2010/7

N2 - In this study, we investigated the cytotoxic effect of perezone, a constituent isolated from the roots of Perezia spp. and of its synthetic isomer isoperezone on the K562 human leukemia cell line. Perezone showed greater cytotoxic effect than isoperezone but both compounds were found to induce cytotoxicity trough a caspase-dependent and a caspase-independent mechanisms; important changes in their light scattering properties, phosphatidylserine translocation and mitochondrial membrane potential disruption were detected by cytometry. The mechanism of death induction of each compound showed interesting concentration-dependent differences. Neither compound induced the apoptosis inducing factor.

AB - In this study, we investigated the cytotoxic effect of perezone, a constituent isolated from the roots of Perezia spp. and of its synthetic isomer isoperezone on the K562 human leukemia cell line. Perezone showed greater cytotoxic effect than isoperezone but both compounds were found to induce cytotoxicity trough a caspase-dependent and a caspase-independent mechanisms; important changes in their light scattering properties, phosphatidylserine translocation and mitochondrial membrane potential disruption were detected by cytometry. The mechanism of death induction of each compound showed interesting concentration-dependent differences. Neither compound induced the apoptosis inducing factor.

KW - Cell death

KW - Cytotoxic

KW - Isoperezone

KW - Perezone

KW - Sesquiterpenes

UR - http://www.scopus.com/inward/record.url?scp=77952426694&partnerID=8YFLogxK

U2 - 10.1016/j.phymed.2009.12.011

DO - 10.1016/j.phymed.2009.12.011

M3 - Artículo

C2 - 20089388

SN - 0944-7113

VL - 17

SP - 614

EP - 620

JO - Phytomedicine

JF - Phytomedicine

IS - 8-9

ER -

Perezone and its isomer isoperezone induce caspase-dependent and caspase-independent cell death

Abstract

Keywords

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