TY - JOUR
T1 - Peptides derived from in vitro gastrointestinal digestion of germinated soybean proteins inhibit human colon cancer cells proliferation and inflammation
AU - González-Montoya, Marcela
AU - Hernández-Ledesma, Blanca
AU - Silván, Jose Manuel
AU - Mora-Escobedo, Rosalva
AU - Martínez-Villaluenga, Cristina
N1 - Publisher Copyright:
© 2017 Elsevier Ltd
PY - 2018/3/1
Y1 - 2018/3/1
N2 - The aim was to investigate the potential of germinated soybean proteins as a source of peptides with anticancer and anti-inflammatory activities produced after simulated gastrointestinal digestion. Protein concentrate from germinated soybean was hydrolysed with pepsin/pancreatin and fractionated by ultrafiltration. Whole digest and fractions >10, 5–10, and <5 kDa caused cytotoxicity to Caco-2, HT-29, HCT-116 human colon cancer cells, and reduced inflammatory response caused by lipopolysaccharide in macrophages RAW 264.7. Antiproliferative and anti-inflammatory effects were generally higher in 5–10 kDa fractions. This fraction was further purified by semi-preparative chromatography and characterised by HPLC-MS/MS. The most potent fraction was mainly composed of β-conglycinin and glycinin fragments rich in glutamine. This is the first report on the anti-cancer and anti-inflammatory effects of newly isolated and identified peptides from germinated soybean released during gastrointestinal digestion. These findings highlight the potential of germination as a process to obtain functional foods or nutraceuticals for colon cancer prevention.
AB - The aim was to investigate the potential of germinated soybean proteins as a source of peptides with anticancer and anti-inflammatory activities produced after simulated gastrointestinal digestion. Protein concentrate from germinated soybean was hydrolysed with pepsin/pancreatin and fractionated by ultrafiltration. Whole digest and fractions >10, 5–10, and <5 kDa caused cytotoxicity to Caco-2, HT-29, HCT-116 human colon cancer cells, and reduced inflammatory response caused by lipopolysaccharide in macrophages RAW 264.7. Antiproliferative and anti-inflammatory effects were generally higher in 5–10 kDa fractions. This fraction was further purified by semi-preparative chromatography and characterised by HPLC-MS/MS. The most potent fraction was mainly composed of β-conglycinin and glycinin fragments rich in glutamine. This is the first report on the anti-cancer and anti-inflammatory effects of newly isolated and identified peptides from germinated soybean released during gastrointestinal digestion. These findings highlight the potential of germination as a process to obtain functional foods or nutraceuticals for colon cancer prevention.
KW - Anti-inflammatory
KW - Antiproliferative
KW - Digestion
KW - Germination
KW - Peptides
KW - Soybean
UR - http://www.scopus.com/inward/record.url?scp=85029145175&partnerID=8YFLogxK
U2 - 10.1016/j.foodchem.2017.09.035
DO - 10.1016/j.foodchem.2017.09.035
M3 - Artículo
C2 - 29037738
SN - 0308-8146
VL - 242
SP - 75
EP - 82
JO - Food Chemistry
JF - Food Chemistry
ER -