Peptides derived from in vitro gastrointestinal digestion of germinated soybean proteins inhibit human colon cancer cells proliferation and inflammation

Marcela González-Montoya, Blanca Hernández-Ledesma, Jose Manuel Silván, Rosalva Mora-Escobedo, Cristina Martínez-Villaluenga

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

© 2017 Elsevier Ltd The aim was to investigate the potential of germinated soybean proteins as a source of peptides with anticancer and anti-inflammatory activities produced after simulated gastrointestinal digestion. Protein concentrate from germinated soybean was hydrolysed with pepsin/pancreatin and fractionated by ultrafiltration. Whole digest and fractions >10, 5–10, and <5 kDa caused cytotoxicity to Caco-2, HT-29, HCT-116 human colon cancer cells, and reduced inflammatory response caused by lipopolysaccharide in macrophages RAW 264.7. Antiproliferative and anti-inflammatory effects were generally higher in 5–10 kDa fractions. This fraction was further purified by semi-preparative chromatography and characterised by HPLC-MS/MS. The most potent fraction was mainly composed of β-conglycinin and glycinin fragments rich in glutamine. This is the first report on the anti-cancer and anti-inflammatory effects of newly isolated and identified peptides from germinated soybean released during gastrointestinal digestion. These findings highlight the potential of germination as a process to obtain functional foods or nutraceuticals for colon cancer prevention.
Original languageAmerican English
Pages (from-to)75-82
Number of pages66
JournalFood Chemistry
DOIs
StatePublished - 1 Mar 2018

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Soybean Proteins
Cell proliferation
anti-inflammatory activity
soy protein
colorectal neoplasms
Colonic Neoplasms
Digestion
cell proliferation
Anti-Inflammatory Agents
inflammation
digestion
Cell Proliferation
peptides
Inflammation
Soybeans
functional foods
Peptides
Pancreatin
soybeans
glycinin

Cite this

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title = "Peptides derived from in vitro gastrointestinal digestion of germinated soybean proteins inhibit human colon cancer cells proliferation and inflammation",
abstract = "{\circledC} 2017 Elsevier Ltd The aim was to investigate the potential of germinated soybean proteins as a source of peptides with anticancer and anti-inflammatory activities produced after simulated gastrointestinal digestion. Protein concentrate from germinated soybean was hydrolysed with pepsin/pancreatin and fractionated by ultrafiltration. Whole digest and fractions >10, 5–10, and <5 kDa caused cytotoxicity to Caco-2, HT-29, HCT-116 human colon cancer cells, and reduced inflammatory response caused by lipopolysaccharide in macrophages RAW 264.7. Antiproliferative and anti-inflammatory effects were generally higher in 5–10 kDa fractions. This fraction was further purified by semi-preparative chromatography and characterised by HPLC-MS/MS. The most potent fraction was mainly composed of β-conglycinin and glycinin fragments rich in glutamine. This is the first report on the anti-cancer and anti-inflammatory effects of newly isolated and identified peptides from germinated soybean released during gastrointestinal digestion. These findings highlight the potential of germination as a process to obtain functional foods or nutraceuticals for colon cancer prevention.",
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Peptides derived from in vitro gastrointestinal digestion of germinated soybean proteins inhibit human colon cancer cells proliferation and inflammation. / González-Montoya, Marcela; Hernández-Ledesma, Blanca; Silván, Jose Manuel; Mora-Escobedo, Rosalva; Martínez-Villaluenga, Cristina.

In: Food Chemistry, 01.03.2018, p. 75-82.

Research output: Contribution to journalArticle

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AU - Hernández-Ledesma, Blanca

AU - Silván, Jose Manuel

AU - Mora-Escobedo, Rosalva

AU - Martínez-Villaluenga, Cristina

PY - 2018/3/1

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AB - © 2017 Elsevier Ltd The aim was to investigate the potential of germinated soybean proteins as a source of peptides with anticancer and anti-inflammatory activities produced after simulated gastrointestinal digestion. Protein concentrate from germinated soybean was hydrolysed with pepsin/pancreatin and fractionated by ultrafiltration. Whole digest and fractions >10, 5–10, and <5 kDa caused cytotoxicity to Caco-2, HT-29, HCT-116 human colon cancer cells, and reduced inflammatory response caused by lipopolysaccharide in macrophages RAW 264.7. Antiproliferative and anti-inflammatory effects were generally higher in 5–10 kDa fractions. This fraction was further purified by semi-preparative chromatography and characterised by HPLC-MS/MS. The most potent fraction was mainly composed of β-conglycinin and glycinin fragments rich in glutamine. This is the first report on the anti-cancer and anti-inflammatory effects of newly isolated and identified peptides from germinated soybean released during gastrointestinal digestion. These findings highlight the potential of germination as a process to obtain functional foods or nutraceuticals for colon cancer prevention.

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