Pathogenicity of isolates of entomopathogenic fungi against spodoptera frugiperda (Lepidoptera: Noctuidae) and Epilachna varivestis (Coleoptera: Coccinellidae)

G. Cipriano García, M. María Berenice González, M. Néstor Bautista

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    Abstract

    In order to obtain native isolates of entomopathogenic fungi in Durango, Mexico, with pathogenicity against Spodoptera frugiperda Fall Armyworm and Epilachna varivestis Mexican Bean Beetle, main pests of maize and beans, respectively, 97 isolates were evaluated, Bb42 and Bb18 of Beauveria bassiana and Ma91 of Metarhizium anisopliae. A bioassay using neonate larvae under completely randomized design of treatments per dose (ANOVA) and Tukey's test were use to measure the insecticidal activity in both insects, mortality data were used to calculate LC50 and LT50. The isolation Bb42 obtained from larvae of S. frugiperda and evaluated against second instar larvae of the same insect showed the highest virulence 96.6% of mortality to rate of 1x109 spores mL-1, with LC50 of 5.92x103 spores mL-1, and lethal time of 3.6 days; isolation Bb18 from beans was highly virulent against neonate larvae of E. varivestis, 93.3% of mortality to 1x109 spores mL-1 with an LC50 of 1.20x106 and lethal time of 5.1 days (Tukey, p ≤ 0.05). In conclusion, the pathogenicity of the isolates was greater when were obtained directly from the insect hosts than from maize and beans soil samples, as well as when it's are obtain from their respective host culture where was find the pest insect.
    Original languageAmerican English
    Pages (from-to)217-222
    Number of pages194
    JournalRevista Colombiana de Entomologia
    StatePublished - 1 Jul 2011

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