Control de bacterias patógenas y hongos de postcosecha con extractos del pigmento de Gibberella zeae (Fusarium graminearum)

Translated title of the contribution: Pathogenic bacteria and postharvest fungi control with pigment extracts from Gibberella zeae (Fusarium graminearum)

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

The objective of this study was to evaluate effects of crude ethanol extract of the reddish purple pigment produced by Gibberella zeae (Fusarium graminearum) in the control of the bacteria Bacillus subtilis, Salmonella typhi, Shigella sp., Staphylococcus aureus, Escherichia coli, the yeast Candida albicans, and the fungi Colletotrichum acutatum, C. fragariae and C. gloeosporioides. The experimental design was completely randomized with a 3×2 factorial arrangement of treatments: temperature (20; 28 °C), pH (6; 8), and solid (PDA; EMA; Czapeck) and liquid (potato broth; malt extract broth; Czapeck broth) culture media. The response variables were growth type and habit of the mycelia, colony color, colony diameter (mm), time (d) of pigment appearance and biomass production (g). With these data an ANOVA was performed and treatment means were compared with orthogonal contrasts. The pigment was extracted with ethanol (96°) and concentrated; 1.6 mg of this extract diluted in 100 μL (16 mg mL-1) was used in pathogenic bacteria and phytopathogenic fungi control tests. The best conditions for growth were 20 °C, pH 6.0 in PDA. The reddish-purple pigment appeared 4 to 9 d after sowing. Fungus growth was less in liquid medium as was red pigment production. After 24 h, inhibition of growth was observed only in the bacteria S. typhi and S. aureus. Analysis of the results revealed antimicrobial activity of the pigment produced by G. zeae against S. typhi and S. aureus, but not against B. subtilis, Shigella sp., E. coli, C. albicans, C. acutatum, C. fragariae or C. gloeosporioides.

Translated title of the contributionPathogenic bacteria and postharvest fungi control with pigment extracts from Gibberella zeae (Fusarium graminearum)
Original languageSpanish
Pages (from-to)691-705
Number of pages15
JournalAgrociencia
Volume47
Issue number7
StatePublished - Nov 2013

Fingerprint

Dive into the research topics of 'Pathogenic bacteria and postharvest fungi control with pigment extracts from Gibberella zeae (Fusarium graminearum)'. Together they form a unique fingerprint.

Cite this