TY - JOUR
T1 - Participation of peripheral P2Y1, P2Y6 and P2Y11 receptors in formalin-induced inflammatory pain in rats
AU - Barragán-Iglesias, Paulino
AU - Mendoza-Garcés, Luis
AU - Pineda-Farias, Jorge Baruch
AU - Solano-Olivares, Verónica
AU - Rodríguez-Silverio, Juan
AU - Flores-Murrieta, Francisco Javier
AU - Granados-Soto, Vinicio
AU - Rocha-González, Héctor Isaac
N1 - Publisher Copyright:
© 2014 Elsevier Inc. All rights reserved.
PY - 2015/1
Y1 - 2015/1
N2 - Metabotropic P2Y receptors subfamily consists of eight functional mammalian receptors. Specifically, P2Y1, P2Y6 and P2Y11 receptors have been described in the sensory nervous system, but their participation, at peripheral level, in behavioral pain models is scarcely understood. This study assessed the role of peripheral P2Y1, P2Y6 and P2Y11 receptors in formalin-induced inflammatory pain. Ipsilateral, but not contralateral peripheral pre-treatment with the endogenous P2Y1 (ADP, 100-1000 nmol/paw), P2Y6 (UDP, 180-300 nmol/paw) and P2Y11 (ATP, 100-1000 nmol/paw), or selective P2Y1 (MRS2365, 0.1-10 nmol/paw), P2Y6 (PSB0474, 0.1-0.10 pmol/paw) and P2Y11 (NF546, 0.3-3 nmol/paw) receptor agonists increased 0.5% formalin-induced flinching behavior. Concordantly, peripheral pre-treatment with the selective P2Y1 (MRS2500, 0.01-10 pmol/paw), P2Y6 (MRS2578, 3-30 nmol/paw) and P2Y11 (NF340, 1-10 nmol/paw) receptor antagonists significantly decreased 1% formalin-induced flinching behavior. Furthermore, the pronociceptive effect of ADP (100 nmol/paw) or MRS2365 (10 nmol/paw), UDP (300 nmol/paw) or PSB0474 (10 pmol/paw) and ATP (1000 nmol/paw) or NF546 (3 nmol/paw) was blocked by the selective P2Y1 (MRS2500, 0.01 nmol/paw), P2Y6 (MRS2578, 3 nmol/paw), and P2Y11 (NF340, 1 nmol/paw) receptor antagonists, respectively. Western blot analysis confirmed the presence of P2Y1 (66 kDa), P2Y6 (36 kDa) and P2Y11 (75 kDa) receptors in dorsal root ganglia (DRG) and sciatic nerve. Results suggest that peripheral activation of P2Y1, P2Y6 and P2Y11 receptors plays a pronociceptive role in formalin-induced pain.
AB - Metabotropic P2Y receptors subfamily consists of eight functional mammalian receptors. Specifically, P2Y1, P2Y6 and P2Y11 receptors have been described in the sensory nervous system, but their participation, at peripheral level, in behavioral pain models is scarcely understood. This study assessed the role of peripheral P2Y1, P2Y6 and P2Y11 receptors in formalin-induced inflammatory pain. Ipsilateral, but not contralateral peripheral pre-treatment with the endogenous P2Y1 (ADP, 100-1000 nmol/paw), P2Y6 (UDP, 180-300 nmol/paw) and P2Y11 (ATP, 100-1000 nmol/paw), or selective P2Y1 (MRS2365, 0.1-10 nmol/paw), P2Y6 (PSB0474, 0.1-0.10 pmol/paw) and P2Y11 (NF546, 0.3-3 nmol/paw) receptor agonists increased 0.5% formalin-induced flinching behavior. Concordantly, peripheral pre-treatment with the selective P2Y1 (MRS2500, 0.01-10 pmol/paw), P2Y6 (MRS2578, 3-30 nmol/paw) and P2Y11 (NF340, 1-10 nmol/paw) receptor antagonists significantly decreased 1% formalin-induced flinching behavior. Furthermore, the pronociceptive effect of ADP (100 nmol/paw) or MRS2365 (10 nmol/paw), UDP (300 nmol/paw) or PSB0474 (10 pmol/paw) and ATP (1000 nmol/paw) or NF546 (3 nmol/paw) was blocked by the selective P2Y1 (MRS2500, 0.01 nmol/paw), P2Y6 (MRS2578, 3 nmol/paw), and P2Y11 (NF340, 1 nmol/paw) receptor antagonists, respectively. Western blot analysis confirmed the presence of P2Y1 (66 kDa), P2Y6 (36 kDa) and P2Y11 (75 kDa) receptors in dorsal root ganglia (DRG) and sciatic nerve. Results suggest that peripheral activation of P2Y1, P2Y6 and P2Y11 receptors plays a pronociceptive role in formalin-induced pain.
KW - Formalin test
KW - Inflammatory pain
KW - P2Y
KW - P2Y
KW - P2Y
KW - Purinergic receptors
UR - http://www.scopus.com/inward/record.url?scp=84912051146&partnerID=8YFLogxK
U2 - 10.1016/j.pbb.2014.11.001
DO - 10.1016/j.pbb.2014.11.001
M3 - Artículo
SN - 0091-3057
VL - 128
SP - 23
EP - 32
JO - Pharmacology Biochemistry and Behavior
JF - Pharmacology Biochemistry and Behavior
ER -