The mouse α-sarcoglycan gene is expressed in muscle cells during differentiation, but its transcriptional regulation is not understood. We have characterized the promoter region of the mouse α-sarcoglycan gene. This region is composed of positive and negative regulatory elements that respond to the myogenic differentiation environment. Accordingly, MyoD transactivates the α-sarcoglycan full-length and the proximal promoter. Chromatin immunoprecipitation assays revealed that MyoD, TFIID, and TFIIB interact with the distal promoter in C2C12 myoblasts, a stage at which the α-SG promoter appears to drive basal activity. In myotubes, such factors are located concomitantly at the distal promoter and at a DNA region around the proximal promoter. In agreement with these results, TFIID and TFIIB co-immunoprecipitate with MyoD. We conclude that the α-SG promoter is activated by MyoD, which interacts with TFIID and TFIIB in a protein complex differentially located at the distal promoter and around the proximal promoter during myogenic cell differentiation. © 2006 Elsevier B.V. All rights reserved.
|Original language||American English|
|Number of pages||215|
|Journal||Biochimica et Biophysica Acta - Gene Structure and Expression|
|State||Published - 1 May 2006|
Delgado-Olguín, P., Recillas-Targa, F., Rosas-Vargas, H., Salamanca, F., & Coral-Vázquez, R. M. (2006). Partial characterization of the mouse α-sarcoglycan promoter and its responsiveness to MyoD. Biochimica et Biophysica Acta - Gene Structure and Expression, 240-246. https://doi.org/10.1016/j.bbaexp.2006.04.007