Partial characterization of the mouse α-sarcoglycan promoter and its responsiveness to MyoD

Paul Delgado-Olguín, Félix Recillas-Targa, Haydeé Rosas-Vargas, Fabio Salamanca, Ramón M. Coral-Vázquez

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Abstract

The mouse α-sarcoglycan gene is expressed in muscle cells during differentiation, but its transcriptional regulation is not understood. We have characterized the promoter region of the mouse α-sarcoglycan gene. This region is composed of positive and negative regulatory elements that respond to the myogenic differentiation environment. Accordingly, MyoD transactivates the α-sarcoglycan full-length and the proximal promoter. Chromatin immunoprecipitation assays revealed that MyoD, TFIID, and TFIIB interact with the distal promoter in C2C12 myoblasts, a stage at which the α-SG promoter appears to drive basal activity. In myotubes, such factors are located concomitantly at the distal promoter and at a DNA region around the proximal promoter. In agreement with these results, TFIID and TFIIB co-immunoprecipitate with MyoD. We conclude that the α-SG promoter is activated by MyoD, which interacts with TFIID and TFIIB in a protein complex differentially located at the distal promoter and around the proximal promoter during myogenic cell differentiation. © 2006 Elsevier B.V. All rights reserved.
Original languageAmerican English
Pages (from-to)240-246
Number of pages215
JournalBiochimica et Biophysica Acta - Gene Structure and Expression
DOIs
StatePublished - 1 May 2006
Externally publishedYes

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