TY - JOUR
T1 - Partial characterization of the mouse α-sarcoglycan promoter and its responsiveness to MyoD
AU - Delgado-Olguín, Paul
AU - Recillas-Targa, Félix
AU - Rosas-Vargas, Haydeé
AU - Salamanca, Fabio
AU - Coral-Vázquez, Ramón M.
N1 - Funding Information:
We thank Georgina Guerrero-Avendaño for the technical assistance and Benoit Bruneau for the critical review of the manuscript. P.D.-O. was supported by Consejo Nacional de Ciencia y Tecnología, México (CONACyT, México) grant 158524, the Dirección General de Estudios de Posgrado, Universidad Nacional Autónoma de México (UNAM), and the Instituto Mexicano del Seguro Social (IMSS) Fellowship Award. R.C.-V. is supported by the Association Française contre les Myopathies grant MNM 2000 Groupe 1. F.R.-T. is supported by CONACyT, México grant 33863-N and 42653-Q, PAPIIT-DGAPA grants IN203200 and IN209403, The Third World Academy of Sciences (TWAS) grant 01-055RG/BIO/LA and the Fundación Miguel Alemán.
PY - 2006/5
Y1 - 2006/5
N2 - The mouse α-sarcoglycan gene is expressed in muscle cells during differentiation, but its transcriptional regulation is not understood. We have characterized the promoter region of the mouse α-sarcoglycan gene. This region is composed of positive and negative regulatory elements that respond to the myogenic differentiation environment. Accordingly, MyoD transactivates the α-sarcoglycan full-length and the proximal promoter. Chromatin immunoprecipitation assays revealed that MyoD, TFIID, and TFIIB interact with the distal promoter in C2C12 myoblasts, a stage at which the α-SG promoter appears to drive basal activity. In myotubes, such factors are located concomitantly at the distal promoter and at a DNA region around the proximal promoter. In agreement with these results, TFIID and TFIIB co-immunoprecipitate with MyoD. We conclude that the α-SG promoter is activated by MyoD, which interacts with TFIID and TFIIB in a protein complex differentially located at the distal promoter and around the proximal promoter during myogenic cell differentiation.
AB - The mouse α-sarcoglycan gene is expressed in muscle cells during differentiation, but its transcriptional regulation is not understood. We have characterized the promoter region of the mouse α-sarcoglycan gene. This region is composed of positive and negative regulatory elements that respond to the myogenic differentiation environment. Accordingly, MyoD transactivates the α-sarcoglycan full-length and the proximal promoter. Chromatin immunoprecipitation assays revealed that MyoD, TFIID, and TFIIB interact with the distal promoter in C2C12 myoblasts, a stage at which the α-SG promoter appears to drive basal activity. In myotubes, such factors are located concomitantly at the distal promoter and at a DNA region around the proximal promoter. In agreement with these results, TFIID and TFIIB co-immunoprecipitate with MyoD. We conclude that the α-SG promoter is activated by MyoD, which interacts with TFIID and TFIIB in a protein complex differentially located at the distal promoter and around the proximal promoter during myogenic cell differentiation.
KW - Core promoter
KW - MyoD transactivation
KW - α-Sarcoglycan promoter
UR - http://www.scopus.com/inward/record.url?scp=33745894175&partnerID=8YFLogxK
U2 - 10.1016/j.bbaexp.2006.04.007
DO - 10.1016/j.bbaexp.2006.04.007
M3 - Artículo
SN - 0167-4781
VL - 1759
SP - 240
EP - 246
JO - Biochimica et Biophysica Acta - Gene Structure and Expression
JF - Biochimica et Biophysica Acta - Gene Structure and Expression
IS - 5
ER -