TY - JOUR
T1 - Parametric characterization of the initial pH effect on the polysaccharides production by Lentinula edodes in submerged culture
AU - García-Cruz, F.
AU - Durán-Páramo, E.
AU - Garín-Aguilar, M. A.
AU - Valencia del Toro, G.
AU - Chairez, I.
N1 - Publisher Copyright:
© 2019
PY - 2020/1
Y1 - 2020/1
N2 - Edible fungus Lentinula edodes (L. edodes) produces exo-polysaccharides (EPS), which have been obtained from submerged mycelia cultures. EPS are bioactive products which can be obtained more efficiently from submerged cultures compared to regular production methods from endo-polyssacharides from the fruiting bodies of fungus cultured on solid medium. A common manner to characterize the production ability of L. edodes is using the kinetic parameters evaluation of a suitable mathematical model which takes into account the effects of diverse experimental conditions. The aim of this study is characterize the production of exo-polysaccharide extracts from submerged cultures of three strains of L. edodes (commercial, L5 and L9) using the estimated parameters of the proposed model. The submerged mycelia cultures have fixed initial pH of 6.5, 4.5 and 3.5. Submerged cultures at an initial pH of 4.5 had maximum amounts of biomass of 10.0–15.4 g L−1. The exo-polysaccharide extracts concentrations are in the range of 0.44–0.97 g L−1. The strain L9 obtained a higher yield biomass-substrate (YX/S = 1.62). At pH 4.5, the commercial strain obtained a higher yield of product-substrate (YP/S = 0.147). If the initial pH is 3.5, the lowest YX/S is obtained in values ranging from 0.85 to 1.21. The maximum growth rate was higher for strain L9 at pH 4.5 with μmax values. The obtained exo-polysaccharide extracts from the three strains have the maximum total carbohydrate amounts of 0.5–0.93 g L−1. A soluble proteins percentage range of 0.16–0.39% is obtained. From the hydrolyzed exo-polysaccharide extracts, glucose, mannose, galactose and arabinose can be identified as the main mono-saccharides. O[sbnd]H bonds, ether bonds, C[sbnd]N bonds and carboxyl bonds are the identified functional groups of the exo-polysaccharides.
AB - Edible fungus Lentinula edodes (L. edodes) produces exo-polysaccharides (EPS), which have been obtained from submerged mycelia cultures. EPS are bioactive products which can be obtained more efficiently from submerged cultures compared to regular production methods from endo-polyssacharides from the fruiting bodies of fungus cultured on solid medium. A common manner to characterize the production ability of L. edodes is using the kinetic parameters evaluation of a suitable mathematical model which takes into account the effects of diverse experimental conditions. The aim of this study is characterize the production of exo-polysaccharide extracts from submerged cultures of three strains of L. edodes (commercial, L5 and L9) using the estimated parameters of the proposed model. The submerged mycelia cultures have fixed initial pH of 6.5, 4.5 and 3.5. Submerged cultures at an initial pH of 4.5 had maximum amounts of biomass of 10.0–15.4 g L−1. The exo-polysaccharide extracts concentrations are in the range of 0.44–0.97 g L−1. The strain L9 obtained a higher yield biomass-substrate (YX/S = 1.62). At pH 4.5, the commercial strain obtained a higher yield of product-substrate (YP/S = 0.147). If the initial pH is 3.5, the lowest YX/S is obtained in values ranging from 0.85 to 1.21. The maximum growth rate was higher for strain L9 at pH 4.5 with μmax values. The obtained exo-polysaccharide extracts from the three strains have the maximum total carbohydrate amounts of 0.5–0.93 g L−1. A soluble proteins percentage range of 0.16–0.39% is obtained. From the hydrolyzed exo-polysaccharide extracts, glucose, mannose, galactose and arabinose can be identified as the main mono-saccharides. O[sbnd]H bonds, ether bonds, C[sbnd]N bonds and carboxyl bonds are the identified functional groups of the exo-polysaccharides.
KW - Fungus Lentinula edodes
KW - Kinetic modeling
KW - Parametric characterization
KW - Polysaccharides
KW - Submerged cultures
UR - http://www.scopus.com/inward/record.url?scp=85075505047&partnerID=8YFLogxK
U2 - 10.1016/j.fbp.2019.10.016
DO - 10.1016/j.fbp.2019.10.016
M3 - Artículo
SN - 0960-3085
VL - 119
SP - 170
EP - 178
JO - Food and Bioproducts Processing
JF - Food and Bioproducts Processing
ER -