TY - JOUR
T1 - PAH removal of high molecular weight by characterized bacterial strains from different organic sources
AU - García-Díaz, César
AU - Ponce-Noyola, Ma Teresa
AU - Esparza-García, Fernando
AU - Rivera-Orduña, Flor
AU - Barrera-Cortés, Josefina
N1 - Funding Information:
Project CB-2010/156837 supported by the Consejo Nacional de Ciencia y Tecnología (CONACyT), México . César García Díaz received grant-aided support from CONACyT. Authors wish to thanks the technical support of Cirino Rojas and Claudia Ivonne Flores Pucheta. Comments and suggestions of the Editor and anonymous referees of the journal are warmly acknowledged since they contributed to substantially improve the manuscript.
PY - 2013/11
Y1 - 2013/11
N2 - This study analyzes the capacity of native bacteria associated with humic acids (HA), sugar cane bagasse (SCB), vermicompost (VC) and the earthworm Eisenia andrei (EaW), to remove polycyclic aromatic hydrocarbons of high molecular weight (HMW-PAH). Bacteria isolation was carried out previous enrichment of the organic sources (OS) with mineral salts and kerosene. An average of 25 bacteria were isolated from each OS and their capacity to remove the PAH, per group (each added at 0.5 O.D), was tested according to a combinatory experimental design with two controls: enriched and non-enriched supernatants of the OS. The bacterial mixture provided by the HA and SCB (44) showed the best performance for degrading the selected PAH. Isolated bacteria associated to these materials were classified into 12 genera, three of which have been previously reported as highly efficient hydrocarbon degraders: Gordonia, Mycobacterium, and Rhodococcus. Regarding the other bacterial genera found, they possess interesting characteristics which include efficient surfactant production capacity, tolerance to high metal concentrations and capacity to reproduce in dry and nutrient-deficient conditions. Therefore, given the characteristics of the isolated hydrocarbonoclastic strains, they could be successfully applied to bioremediation by bioaugmentation of soil co-contaminated with hydrocarbons, metals and even pesticides.
AB - This study analyzes the capacity of native bacteria associated with humic acids (HA), sugar cane bagasse (SCB), vermicompost (VC) and the earthworm Eisenia andrei (EaW), to remove polycyclic aromatic hydrocarbons of high molecular weight (HMW-PAH). Bacteria isolation was carried out previous enrichment of the organic sources (OS) with mineral salts and kerosene. An average of 25 bacteria were isolated from each OS and their capacity to remove the PAH, per group (each added at 0.5 O.D), was tested according to a combinatory experimental design with two controls: enriched and non-enriched supernatants of the OS. The bacterial mixture provided by the HA and SCB (44) showed the best performance for degrading the selected PAH. Isolated bacteria associated to these materials were classified into 12 genera, three of which have been previously reported as highly efficient hydrocarbon degraders: Gordonia, Mycobacterium, and Rhodococcus. Regarding the other bacterial genera found, they possess interesting characteristics which include efficient surfactant production capacity, tolerance to high metal concentrations and capacity to reproduce in dry and nutrient-deficient conditions. Therefore, given the characteristics of the isolated hydrocarbonoclastic strains, they could be successfully applied to bioremediation by bioaugmentation of soil co-contaminated with hydrocarbons, metals and even pesticides.
KW - Bioremediation
KW - Cellulosic organic materials
KW - Humic acids
KW - PAH
KW - Sugar cane bagasse
KW - Vermicompost
UR - http://www.scopus.com/inward/record.url?scp=84884277520&partnerID=8YFLogxK
U2 - 10.1016/j.ibiod.2013.08.016
DO - 10.1016/j.ibiod.2013.08.016
M3 - Artículo
SN - 0964-8305
VL - 85
SP - 311
EP - 322
JO - International Biodeterioration and Biodegradation
JF - International Biodeterioration and Biodegradation
ER -