Abstract
It was evaluated the growth of Azadirachta indica cell suspension in different conditions of oxygen delivery in Erlenmeyer shake flask. Oxygen transfer rate (OTR, kg O2 m-3 day-1) for the closures utilized were: silicone foam (1.04), cotton (0.58), and aluminum foil (0.07). A. indica cells growing during 6 weeks of subculture showed that lower OTR reduced cell viability, the pH of broth medium, and Azadirachtins production. While, higher OTR induced the formation of aggregates. Using a stirred tank bioreactor, it was determined that A. indica cells had an oxygen consumption of 0.100 kg O2 kg DW-1 day-1, a higher value than other plant cell cultures. These results show that OTR generated in Erlenmeyer shake flasks is lower to oxygen uptake rate of A. indica cells and it is a limiting factor to grow this plant.
| Translated title of the contribution | Oxygen limitations to growaz Adirachta indica cell culture in shake flasks |
|---|---|
| Original language | English |
| Pages (from-to) | 343-352 |
| Number of pages | 10 |
| Journal | Revista Mexicana de Ingeniera Quimica |
| Volume | 10 |
| Issue number | 3 |
| State | Published - Dec 2011 |
Keywords
- Azadirachta indica cell culture
- Closures
- Oxygen transfer esistance
- Oxygen transfer rate
- Shake flask