TY - JOUR
T1 - Oxidative stress induced in Hyalella azteca by an effluent from a NSAID-manufacturing plant in Mexico
AU - Novoa-Luna, Karen Adriana
AU - Romero-Romero, Rubí
AU - Natividad-Rangel, Reyna
AU - Galar-Martínez, Marcela
AU - SanJuan-Reyes, Nely
AU - García-Medina, Sandra
AU - Martínez-Vieyra, Catalina
AU - Neri-Cruz, Nadia
AU - Gómez-Oliván, Leobardo Manuel
N1 - Publisher Copyright:
© 2016, Springer Science+Business Media New York.
PY - 2016/9/1
Y1 - 2016/9/1
N2 - Production in the pharmaceutical industry has increased and along with it, the amount of wastewater of various characteristics and contaminant concentrations. The main chemicals in these effluents are solvents, detergents, disinfectants—such as sodium hypochlorite (NaClO)—and pharmaceutical products, all of which are potentially ecotoxic. Therefore, this study aimed to evaluate the oxidative stress induced in the amphipod Hyalella azteca by the effluent from a nonsteroidal anti-inflammatory drug (NSAID)-manufacturing plant. The median lethal concentration (72 h-LC50) was determined and H. azteca were exposed to the lowest observed adverse effect level (0.0732 %) for 12, 24, 48 and 72 h, and biomarkers of oxidative stress were evaluated [hydroperoxide content (HPC), lipid peroxidation (LPX), protein carbonyl content (PCC), and the activity of the superoxidant enzymes superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx)]. Statistically significant increases with respect to the control group (P < 0.05) were observed in HPC, LPX and PCC in H. azteca at all exposure times. Antioxidant enzymes activity SOD, CAT and GPx activity also increased significantly (P < 0.05) with respect to the control group. In conclusion, the industrial effluent analyzed in the present study contains NSAIDs and NaClO, and induces oxidative stress in H. azteca.
AB - Production in the pharmaceutical industry has increased and along with it, the amount of wastewater of various characteristics and contaminant concentrations. The main chemicals in these effluents are solvents, detergents, disinfectants—such as sodium hypochlorite (NaClO)—and pharmaceutical products, all of which are potentially ecotoxic. Therefore, this study aimed to evaluate the oxidative stress induced in the amphipod Hyalella azteca by the effluent from a nonsteroidal anti-inflammatory drug (NSAID)-manufacturing plant. The median lethal concentration (72 h-LC50) was determined and H. azteca were exposed to the lowest observed adverse effect level (0.0732 %) for 12, 24, 48 and 72 h, and biomarkers of oxidative stress were evaluated [hydroperoxide content (HPC), lipid peroxidation (LPX), protein carbonyl content (PCC), and the activity of the superoxidant enzymes superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx)]. Statistically significant increases with respect to the control group (P < 0.05) were observed in HPC, LPX and PCC in H. azteca at all exposure times. Antioxidant enzymes activity SOD, CAT and GPx activity also increased significantly (P < 0.05) with respect to the control group. In conclusion, the industrial effluent analyzed in the present study contains NSAIDs and NaClO, and induces oxidative stress in H. azteca.
KW - Amphipod
KW - Hyalella azteca
KW - NaClO
KW - Nonsteroidal anti-inflammatory drugs
KW - Oxidative stress
UR - http://www.scopus.com/inward/record.url?scp=84973137366&partnerID=8YFLogxK
U2 - 10.1007/s10646-016-1682-2
DO - 10.1007/s10646-016-1682-2
M3 - Artículo
SN - 0963-9292
VL - 25
SP - 1288
EP - 1304
JO - Ecotoxicology
JF - Ecotoxicology
IS - 7
ER -