Oxidation of solid paraffin (C<inf>11-40</inf>) by Pseudomonas aeruginosa MGP-1

Rosa Salgado-Brito, M. Isabel Neria, Ana M. Mesta-Howard, Francisco Díaz Cedillo, En Tao Wang

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Isolated from oil-contaminated soil, Pseudomonas aeruginosa strain MGP-1 was identified by 16S rDNA sequence analysis and phenotypic characterisation. This strain was highly competent in medium and in soils, grew and survived well under adverse conditions. MGP-1 did not produce the pathogenic factor pyocyanin and used paraffin (C11-40) as sole carbon source. It degraded eicosane (C20) more efficiently than other n-alkanes, removing 60% within 16 days. An alkB gene and intermediate metabolites of mono-alcohol and lipid acid corresponding to the oxidised paraffin were detected in the cell-free extracts of MGP-1, indicating that it possessed a mono-terminal oxidation. This is the first report to demonstrate that a Pseudomonas strain is able to degrade paraffin with more than 18 carbon atoms by mono-terminal oxidation. Considering all the results, MGP-1 could be designed as a soil-born, highly competent, long chain n-alkane degrading bacterium with low or perhaps without pathogenicity.
Original languageAmerican English
Pages (from-to)321-328
Number of pages288
JournalAnnals of Microbiology
DOIs
StatePublished - 1 Jan 2007

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Paraffin
Pseudomonas aeruginosa
Alkanes
Soil
Carbon
Pyocyanine
Ribosomal DNA
Pseudomonas
Cell Extracts
Sequence Analysis
Virulence
Oils
Alcohols
Bacteria
Lipids
Acids
Genes
eicosane

Cite this

Salgado-Brito, Rosa ; Isabel Neria, M. ; Mesta-Howard, Ana M. ; Cedillo, Francisco Díaz ; Wang, En Tao. / Oxidation of solid paraffin (C<inf>11-40</inf>) by Pseudomonas aeruginosa MGP-1. In: Annals of Microbiology. 2007 ; pp. 321-328.
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abstract = "Isolated from oil-contaminated soil, Pseudomonas aeruginosa strain MGP-1 was identified by 16S rDNA sequence analysis and phenotypic characterisation. This strain was highly competent in medium and in soils, grew and survived well under adverse conditions. MGP-1 did not produce the pathogenic factor pyocyanin and used paraffin (C11-40) as sole carbon source. It degraded eicosane (C20) more efficiently than other n-alkanes, removing 60{\%} within 16 days. An alkB gene and intermediate metabolites of mono-alcohol and lipid acid corresponding to the oxidised paraffin were detected in the cell-free extracts of MGP-1, indicating that it possessed a mono-terminal oxidation. This is the first report to demonstrate that a Pseudomonas strain is able to degrade paraffin with more than 18 carbon atoms by mono-terminal oxidation. Considering all the results, MGP-1 could be designed as a soil-born, highly competent, long chain n-alkane degrading bacterium with low or perhaps without pathogenicity.",
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Oxidation of solid paraffin (C<inf>11-40</inf>) by Pseudomonas aeruginosa MGP-1. / Salgado-Brito, Rosa; Isabel Neria, M.; Mesta-Howard, Ana M.; Cedillo, Francisco Díaz; Wang, En Tao.

In: Annals of Microbiology, 01.01.2007, p. 321-328.

Research output: Contribution to journalArticle

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