Outdoor production of a nitrogen-fixing photosynthetic microbial consortium in batch and semi-continuous culture in a flat-panel airlift photobioreactor

Dulce Jazmín Hernández Melchor, Eliseo Cristiani Urbina, Ronald Ferrera Cerrato, José Fernando Esparza García, Rosa Olivia Cañizares Villanueva

Research output: Contribution to journalArticle

Abstract

© 2017, Centro de Ciencias de la Atmosfera, UNAM. All rights reserved. Cyanobacteria are oxygenic phototrophic organisms that have the capacity to form consortia. One strategy that has been used to improve the productivity of cyanobacteria and algae in photobioreactors is to produce them in semicontinuous cultures. In our research group, a photosynthetic microbial consortium (MC) with nitrogen fixing capacity was studied. It consists mainly of cyanobacteria and could potentially be used as a biofertilizer. The aim of this study was to evaluate the capability to produce biomass of MC in two flat-panel airlift photobioreactors (PBR) (50 y 150 L) in batch culture, and in semicontinuous culture in a PBR with 150 L capacity, outdoors. The maximum biomass concentration in batch culture was obtained in the PBR of 150 L, with a value of 600 mg/L, in comparison with the biomass concentration obtained in the PBR of 50 L, which was equal to 385 mg/L. The biomass productivity at the end of the batch culture in both PBR (50 and 150 L) was 0.77 and 1.19 mg/L/h, respectively. The semicontinuous culture of CM was operated during three cycles (7, 7 and 5 d, respectively), attaining an increase in the biomass productivity following each cycle, with values of 1.86, 1.94 and 2.94 mg/L/h, respectively. Similarly, the maximum values of the nitrogenase activity were obtained at the end of each cycle with values of 4963, 5366 and 5689 nmol ethene/g dry weight/h, respectively. These were higher than the values obtained in batch cultures. In conclusion, it was possible to produce the MC in the PBR in batch and semicontinuous mode outdoors, without affecting the nitrogen fixation capacity.
Original languageAmerican English
Pages (from-to)73-81
Number of pages9
JournalRevista Internacional de Contaminacion Ambiental
DOIs
StatePublished - 1 Jan 2017

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Photobioreactors
Nitrogen
Biomass
nitrogen
biomass
cyanobacterium
Productivity
productivity
Nitrogen fixation
biofertilizer
nitrogen fixation
Algae
ethylene
alga

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Hernández Melchor, Dulce Jazmín ; Cristiani Urbina, Eliseo ; Ferrera Cerrato, Ronald ; Esparza García, José Fernando ; Cañizares Villanueva, Rosa Olivia. / Outdoor production of a nitrogen-fixing photosynthetic microbial consortium in batch and semi-continuous culture in a flat-panel airlift photobioreactor. In: Revista Internacional de Contaminacion Ambiental. 2017 ; pp. 73-81.
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Outdoor production of a nitrogen-fixing photosynthetic microbial consortium in batch and semi-continuous culture in a flat-panel airlift photobioreactor. / Hernández Melchor, Dulce Jazmín; Cristiani Urbina, Eliseo; Ferrera Cerrato, Ronald; Esparza García, José Fernando; Cañizares Villanueva, Rosa Olivia.

In: Revista Internacional de Contaminacion Ambiental, 01.01.2017, p. 73-81.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Outdoor production of a nitrogen-fixing photosynthetic microbial consortium in batch and semi-continuous culture in a flat-panel airlift photobioreactor

AU - Hernández Melchor, Dulce Jazmín

AU - Cristiani Urbina, Eliseo

AU - Ferrera Cerrato, Ronald

AU - Esparza García, José Fernando

AU - Cañizares Villanueva, Rosa Olivia

PY - 2017/1/1

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AB - © 2017, Centro de Ciencias de la Atmosfera, UNAM. All rights reserved. Cyanobacteria are oxygenic phototrophic organisms that have the capacity to form consortia. One strategy that has been used to improve the productivity of cyanobacteria and algae in photobioreactors is to produce them in semicontinuous cultures. In our research group, a photosynthetic microbial consortium (MC) with nitrogen fixing capacity was studied. It consists mainly of cyanobacteria and could potentially be used as a biofertilizer. The aim of this study was to evaluate the capability to produce biomass of MC in two flat-panel airlift photobioreactors (PBR) (50 y 150 L) in batch culture, and in semicontinuous culture in a PBR with 150 L capacity, outdoors. The maximum biomass concentration in batch culture was obtained in the PBR of 150 L, with a value of 600 mg/L, in comparison with the biomass concentration obtained in the PBR of 50 L, which was equal to 385 mg/L. The biomass productivity at the end of the batch culture in both PBR (50 and 150 L) was 0.77 and 1.19 mg/L/h, respectively. The semicontinuous culture of CM was operated during three cycles (7, 7 and 5 d, respectively), attaining an increase in the biomass productivity following each cycle, with values of 1.86, 1.94 and 2.94 mg/L/h, respectively. Similarly, the maximum values of the nitrogenase activity were obtained at the end of each cycle with values of 4963, 5366 and 5689 nmol ethene/g dry weight/h, respectively. These were higher than the values obtained in batch cultures. In conclusion, it was possible to produce the MC in the PBR in batch and semicontinuous mode outdoors, without affecting the nitrogen fixation capacity.

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DO - 10.20937/RICA.2017.33.esp01.07

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SN - 0188-4999

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