Online determination of structural properties and observation of deviations from power law behavior

Agnès Rolland-Sabaté, Maria Guadalupe Mendez-Montealvo, Paul Colonna, Véronique Planchot

Research output: Contribution to journalArticle

60 Citations (Scopus)

Abstract

Synthetic amyloses, pullulans, phytoglycogen, rabbit liver glycogen, oyster glycogen, and dextrans were studied using high-performance size-exclusion chromatography combined with multiangle laser light scattering (MALLS) and online quasi-elastic light scattering (QELS), which provided the R H distributions up to 65 nm. Different structural parameters were extracted from entire molar mass distributions, including the slope of the log-log plot of R HI versus M i and the ρ i = R Gi /R Hi ratio. This approach enabled to observe deviations from the De Gennes scaling law concept. Evidences that the power laws do not obey the general universality were furnished by the observation of strong deviations in the relation between radii and molar masses for the branched polysaccharides, a decrease of ρ-parameter with molar mass toward values much lower than theoretically expected, and the fact that relation between ρ-parameter and apparent segment density did not show the expected power law decrease with an exponent of 1/3. The universality of scaling behavior seems no longer to be realized if structural heterogeneity governs the system. © 2008 American Chemical Society.
Original languageAmerican English
Pages (from-to)1719-1730
Number of pages12
JournalBiomacromolecules
DOIs
StatePublished - 1 Jul 2008

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Molar mass
Structural properties
Light scattering
Amylose
Liver Glycogen
Elastic scattering
Dextran
Size exclusion chromatography
Scaling laws
Polysaccharides
Dextrans
Glycogen
Liver
Lasers

Cite this

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title = "Online determination of structural properties and observation of deviations from power law behavior",
abstract = "Synthetic amyloses, pullulans, phytoglycogen, rabbit liver glycogen, oyster glycogen, and dextrans were studied using high-performance size-exclusion chromatography combined with multiangle laser light scattering (MALLS) and online quasi-elastic light scattering (QELS), which provided the R H distributions up to 65 nm. Different structural parameters were extracted from entire molar mass distributions, including the slope of the log-log plot of R HI versus M i and the ρ i = R Gi /R Hi ratio. This approach enabled to observe deviations from the De Gennes scaling law concept. Evidences that the power laws do not obey the general universality were furnished by the observation of strong deviations in the relation between radii and molar masses for the branched polysaccharides, a decrease of ρ-parameter with molar mass toward values much lower than theoretically expected, and the fact that relation between ρ-parameter and apparent segment density did not show the expected power law decrease with an exponent of 1/3. The universality of scaling behavior seems no longer to be realized if structural heterogeneity governs the system. {\circledC} 2008 American Chemical Society.",
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year = "2008",
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doi = "10.1021/bm7013119",
language = "American English",
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Online determination of structural properties and observation of deviations from power law behavior. / Rolland-Sabaté, Agnès; Mendez-Montealvo, Maria Guadalupe; Colonna, Paul; Planchot, Véronique.

In: Biomacromolecules, 01.07.2008, p. 1719-1730.

Research output: Contribution to journalArticle

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T1 - Online determination of structural properties and observation of deviations from power law behavior

AU - Rolland-Sabaté, Agnès

AU - Mendez-Montealvo, Maria Guadalupe

AU - Colonna, Paul

AU - Planchot, Véronique

PY - 2008/7/1

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N2 - Synthetic amyloses, pullulans, phytoglycogen, rabbit liver glycogen, oyster glycogen, and dextrans were studied using high-performance size-exclusion chromatography combined with multiangle laser light scattering (MALLS) and online quasi-elastic light scattering (QELS), which provided the R H distributions up to 65 nm. Different structural parameters were extracted from entire molar mass distributions, including the slope of the log-log plot of R HI versus M i and the ρ i = R Gi /R Hi ratio. This approach enabled to observe deviations from the De Gennes scaling law concept. Evidences that the power laws do not obey the general universality were furnished by the observation of strong deviations in the relation between radii and molar masses for the branched polysaccharides, a decrease of ρ-parameter with molar mass toward values much lower than theoretically expected, and the fact that relation between ρ-parameter and apparent segment density did not show the expected power law decrease with an exponent of 1/3. The universality of scaling behavior seems no longer to be realized if structural heterogeneity governs the system. © 2008 American Chemical Society.

AB - Synthetic amyloses, pullulans, phytoglycogen, rabbit liver glycogen, oyster glycogen, and dextrans were studied using high-performance size-exclusion chromatography combined with multiangle laser light scattering (MALLS) and online quasi-elastic light scattering (QELS), which provided the R H distributions up to 65 nm. Different structural parameters were extracted from entire molar mass distributions, including the slope of the log-log plot of R HI versus M i and the ρ i = R Gi /R Hi ratio. This approach enabled to observe deviations from the De Gennes scaling law concept. Evidences that the power laws do not obey the general universality were furnished by the observation of strong deviations in the relation between radii and molar masses for the branched polysaccharides, a decrease of ρ-parameter with molar mass toward values much lower than theoretically expected, and the fact that relation between ρ-parameter and apparent segment density did not show the expected power law decrease with an exponent of 1/3. The universality of scaling behavior seems no longer to be realized if structural heterogeneity governs the system. © 2008 American Chemical Society.

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