TY - JOUR
T1 - NSAID-manufacturing plant effluent induces geno- and cytotoxicity in common carp (Cyprinus carpio)
AU - SanJuan-Reyes, Nely
AU - Gómez-Oliván, Leobardo Manuel
AU - Galar-Martínez, Marcela
AU - García-Medina, Sandra
AU - Islas-Flores, Hariz
AU - González-González, Edgar David
AU - Cardoso-Vera, Jesús Daniel
AU - Jiménez-Vargas, Juan Manuel
N1 - Publisher Copyright:
© 2015 Elsevier B.V.
PY - 2015/10/5
Y1 - 2015/10/5
N2 - The pharmaceutical industry generates wastewater discharges of varying characteristics and contaminant concentrations depending on the nature of the production process. The main chemicals present in these effluents are solvents, detergents, disinfectants - such as sodium hypochlorite (NaClO) - and pharmaceutical products, all of which are potentially ecotoxic. Therefore, this study aimed to evaluate the geno- and cytotoxicity induced in the common carp Cyprinus carpio by the effluent emanating from a nonsteroidal anti-inflammatory drug (NSAID)-manufacturing plant. Carp were exposed to the lowest observed adverse effect level (LOAEL, 0.1173%) for 12, 24, 48, 72 and 96. h, and biomarkers of genotoxicity (comet assay and micronucleus test) and cytotoxicity (caspase-3 activity and TUNEL assay) were evaluated. A significant increase with respect to the control group (p<. 0.05) occurred with all biomarkers from 24. h on. Significant positive correlations were found between NSAID concentrations and biomarkers of geno- and cytotoxicity, as well as among geno- and cytotoxicity biomarkers. In conclusion, exposure to this industrial effluent induces geno- and cytotoxicity in blood of C. carpio.
AB - The pharmaceutical industry generates wastewater discharges of varying characteristics and contaminant concentrations depending on the nature of the production process. The main chemicals present in these effluents are solvents, detergents, disinfectants - such as sodium hypochlorite (NaClO) - and pharmaceutical products, all of which are potentially ecotoxic. Therefore, this study aimed to evaluate the geno- and cytotoxicity induced in the common carp Cyprinus carpio by the effluent emanating from a nonsteroidal anti-inflammatory drug (NSAID)-manufacturing plant. Carp were exposed to the lowest observed adverse effect level (LOAEL, 0.1173%) for 12, 24, 48, 72 and 96. h, and biomarkers of genotoxicity (comet assay and micronucleus test) and cytotoxicity (caspase-3 activity and TUNEL assay) were evaluated. A significant increase with respect to the control group (p<. 0.05) occurred with all biomarkers from 24. h on. Significant positive correlations were found between NSAID concentrations and biomarkers of geno- and cytotoxicity, as well as among geno- and cytotoxicity biomarkers. In conclusion, exposure to this industrial effluent induces geno- and cytotoxicity in blood of C. carpio.
KW - Apoptosis
KW - DNA damage
KW - Effluent
KW - Pharmaceutical industry
UR - http://www.scopus.com/inward/record.url?scp=84930210079&partnerID=8YFLogxK
U2 - 10.1016/j.scitotenv.2015.05.088
DO - 10.1016/j.scitotenv.2015.05.088
M3 - Artículo
SN - 0048-9697
VL - 530-531
SP - 1
EP - 10
JO - Science of the Total Environment
JF - Science of the Total Environment
ER -