Novel toxicity of native and HD Bacillus thuringiensis strains against to the sugarcane borer Diatraea saccharalis

Ninfa M. Rosas-García, Benito Pereyra-Alférez, Katiushka Arévalo Niño, Luis J. Gaĺn-Wong, Lilia H. Morales-Ramos

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Abstract

Diatraea saccharalis (F.) (Lepidoptera: Pyralidae) is a pest that causes great economic losses to sugarcane producers in Mexico. In order to obtain alternatives for control of this pest, several Bacillus thuringiensis strains (native and from the Howard Dulmage collection) were tested. In bioassays, strains HD-133, HD-551, GM-7, GM-10, and GM-34 caused more than 50% mortality with a 50 μg/ml spore-crystal complex concentration, and were selected as toxic strains. The lowest LC 50 value corresponded to GM-34 (33.21 μg/ml). Cry1B and cry1C genes were detected by PCR analysis in the toxic strains. HD-133 and GM-10 habored cry1C gene, HD-551 and GM-7 strains harbored cry1B gene, while GM34 strain did not contain cry1B nor cry1C. An additional PCR analysis was performed to detect cry1A-type genes. All the toxic strains habor at least one cry1A-type gene. Immunoblotting revealed that all strains cross-reacted with an antiCry1A, and only the HD-551 gave a positive signal with antiCry1B polyclonal antisera. GM-7 crystal protein showed no cross-reaction with polyclonal Cry1B antiserum. The toxicity of these strains may be related to some member of the Cry1A toxin class.
Original languageAmerican English
Pages (from-to)455-465
Number of pages408
JournalBioControl
DOIs
StatePublished - 1 Aug 2004

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