TY - JOUR
T1 - Non-synonymous polymorphisms in candidate gene associated with growth traits in Channel catfish (Ictalurus punctatus, Rafinesque, 1818)
AU - Suárez-Salgado, Diana
AU - Parra-Bracamonte, Gaspar Manuel
AU - Benavides-González, Flaviano
AU - Alfaro, Isidro O.Montelongo
AU - Rincón, Ana María Sifuentes
AU - Moreno-Medina, Victor Ricardo
AU - De la Rosa-Reyna, Xochitl Fabiola
N1 - Publisher Copyright:
© 2019, Springer Nature B.V.
PY - 2020/1/1
Y1 - 2020/1/1
N2 - Resequencing of Myostatin, Growth Hormone, Follistatin-A-like, Insulin-like Growth Factor I (IGF-I) and Myogenin (MYOG) genes was completed to discover novel genetic variations and assess non synonymous (ns) polymorphisms (SNPs) effect on growth related traits of channel catfish. Wild and farmed animals were used as a discovering population. Resequencing lead to the identification of 59 new variants in the five analyzed genes; 66% found in introns and 34% in coding regions. From coding regions, 14 variants were synonyms and six were ns variations. A mutation rate of one in 129 bp was estimated. Four ns variations were selected for validation and association analysis. In IGF-I two ns polymorphisms, at IGF-I19 the G wild type allele was fixed in population and for IGF-I63 the C allele had a frequency of 0.972 and for mutate allele G of 0.027. In MYOG two ns SNPs were assessed. MYOG131 presented a frequency of alleles T and A, of 0.754 and 0.246, respectively and MYOG233, with a frequency of G and C of 0.775 and 0.225, respectively. Only MYOG131 (g.529T>A) was significantly associated (P < 0.04) to some growth traits. Results suggest MYOG131 g.529T>A as candidate locus for genetic enhancement of growth traits in channel catfish.
AB - Resequencing of Myostatin, Growth Hormone, Follistatin-A-like, Insulin-like Growth Factor I (IGF-I) and Myogenin (MYOG) genes was completed to discover novel genetic variations and assess non synonymous (ns) polymorphisms (SNPs) effect on growth related traits of channel catfish. Wild and farmed animals were used as a discovering population. Resequencing lead to the identification of 59 new variants in the five analyzed genes; 66% found in introns and 34% in coding regions. From coding regions, 14 variants were synonyms and six were ns variations. A mutation rate of one in 129 bp was estimated. Four ns variations were selected for validation and association analysis. In IGF-I two ns polymorphisms, at IGF-I19 the G wild type allele was fixed in population and for IGF-I63 the C allele had a frequency of 0.972 and for mutate allele G of 0.027. In MYOG two ns SNPs were assessed. MYOG131 presented a frequency of alleles T and A, of 0.754 and 0.246, respectively and MYOG233, with a frequency of G and C of 0.775 and 0.225, respectively. Only MYOG131 (g.529T>A) was significantly associated (P < 0.04) to some growth traits. Results suggest MYOG131 g.529T>A as candidate locus for genetic enhancement of growth traits in channel catfish.
KW - Follistatin-A-like
KW - GH
KW - Growth rate
KW - IGF-I
KW - Myogenin
KW - Myostatin
KW - RFLP
KW - SNP
KW - Sequencing
UR - http://www.scopus.com/inward/record.url?scp=85074145184&partnerID=8YFLogxK
U2 - 10.1007/s11033-019-05110-0
DO - 10.1007/s11033-019-05110-0
M3 - Artículo
C2 - 31576511
AN - SCOPUS:85074145184
SN - 0301-4851
VL - 47
SP - 87
EP - 95
JO - Molecular Biology Reports
JF - Molecular Biology Reports
IS - 1
ER -