NMR-Based Metabonomic Analysis of Physiological Responses to Starvation and Refeeding in the Rat

José I. Serrano-Contreras, Isabel García-Pérez, María E. Meléndez-Camargo, L. Gerardo Zepeda

Research output: Contribution to journalArticleResearchpeer-review

4 Citations (Scopus)

Abstract

© 2016 American Chemical Society. Starvation is a postabsorptive condition derived from a limitation on food resources by external factors. Energy homeostasis is maintained under this condition by using sources other than glucose via adaptive mechanisms. After refeeding, when food is available, other adaptive processes are linked to energy balance. However, less has been reported about the physiological mechanisms present as a result of these conditions, considering the rat as a supraorganism. Metabolic profiling using 1H nuclear magnetic resonance spectroscopy was used to characterize the physiological metabolic differences in urine specimens collected under starved, refed, and recovered conditions. In addition, because starvation induced lack of faecal production and not all animals produced faeces during refeeding, 24 h pooled faecal water samples were also analyzed. Urinary metabolites upregulated by starvation included 2-butanamidoacetate, 3-hydroxyisovalerate, ketoleucine, methylmalonate, p-cresyl glucuronide, p-cresyl sulfate, phenylacetylglycine, pseudouridine, creatinine, taurine, and N-acetyl glycoprotein, which were related to renal and skeletal muscle function, β-oxidation, turnover of proteins and RNA, and host-microbial interactions. Food-derived metabolites, including gut microbial cometabolites, and tricarboxylic acid cycle intermediates were upregulated under refed and recovered conditions, which characterized anabolic urinary metabotypes. The upregulation of creatine and pantothenate indicated an absorptive state after refeeding. Fecal short chain fatty acids, 3-(3-hydroxyphenyl)propionate, lactate, and acetoin provided additional information about the combinatorial metabolism between the host and gut microbiota. This investigation contributes to allow a deeper understanding of physiological responses associated with starvation and refeeding.
Original languageAmerican English
Pages (from-to)3241-3254
Number of pages2915
JournalJournal of Proteome Research
DOIs
StatePublished - 2 Sep 2016

Fingerprint

Metabolomics
Starvation
Rats
Nuclear magnetic resonance
Metabolites
Pseudouridine
Acetoin
Food
Creatine
Volatile Fatty Acids
Taurine
Glucuronides
Microbial Interactions
Energy balance
Metabolism
Nuclear magnetic resonance spectroscopy
Sulfates
Citric Acid Cycle
Muscle
Lactic Acid

Cite this

Serrano-Contreras, José I. ; García-Pérez, Isabel ; Meléndez-Camargo, María E. ; Gerardo Zepeda, L. / NMR-Based Metabonomic Analysis of Physiological Responses to Starvation and Refeeding in the Rat. In: Journal of Proteome Research. 2016 ; pp. 3241-3254.
@article{e9db2e604f674ada899f57fd657fcefa,
title = "NMR-Based Metabonomic Analysis of Physiological Responses to Starvation and Refeeding in the Rat",
abstract = "{\circledC} 2016 American Chemical Society. Starvation is a postabsorptive condition derived from a limitation on food resources by external factors. Energy homeostasis is maintained under this condition by using sources other than glucose via adaptive mechanisms. After refeeding, when food is available, other adaptive processes are linked to energy balance. However, less has been reported about the physiological mechanisms present as a result of these conditions, considering the rat as a supraorganism. Metabolic profiling using 1H nuclear magnetic resonance spectroscopy was used to characterize the physiological metabolic differences in urine specimens collected under starved, refed, and recovered conditions. In addition, because starvation induced lack of faecal production and not all animals produced faeces during refeeding, 24 h pooled faecal water samples were also analyzed. Urinary metabolites upregulated by starvation included 2-butanamidoacetate, 3-hydroxyisovalerate, ketoleucine, methylmalonate, p-cresyl glucuronide, p-cresyl sulfate, phenylacetylglycine, pseudouridine, creatinine, taurine, and N-acetyl glycoprotein, which were related to renal and skeletal muscle function, β-oxidation, turnover of proteins and RNA, and host-microbial interactions. Food-derived metabolites, including gut microbial cometabolites, and tricarboxylic acid cycle intermediates were upregulated under refed and recovered conditions, which characterized anabolic urinary metabotypes. The upregulation of creatine and pantothenate indicated an absorptive state after refeeding. Fecal short chain fatty acids, 3-(3-hydroxyphenyl)propionate, lactate, and acetoin provided additional information about the combinatorial metabolism between the host and gut microbiota. This investigation contributes to allow a deeper understanding of physiological responses associated with starvation and refeeding.",
author = "Serrano-Contreras, {Jos{\'e} I.} and Isabel Garc{\'i}a-P{\'e}rez and Mel{\'e}ndez-Camargo, {Mar{\'i}a E.} and {Gerardo Zepeda}, L.",
year = "2016",
month = "9",
day = "2",
doi = "10.1021/acs.jproteome.6b00433",
language = "American English",
pages = "3241--3254",
journal = "Journal of Proteome Research",
issn = "1535-3893",
publisher = "American Chemical Society",

}

NMR-Based Metabonomic Analysis of Physiological Responses to Starvation and Refeeding in the Rat. / Serrano-Contreras, José I.; García-Pérez, Isabel; Meléndez-Camargo, María E.; Gerardo Zepeda, L.

In: Journal of Proteome Research, 02.09.2016, p. 3241-3254.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - NMR-Based Metabonomic Analysis of Physiological Responses to Starvation and Refeeding in the Rat

AU - Serrano-Contreras, José I.

AU - García-Pérez, Isabel

AU - Meléndez-Camargo, María E.

AU - Gerardo Zepeda, L.

PY - 2016/9/2

Y1 - 2016/9/2

N2 - © 2016 American Chemical Society. Starvation is a postabsorptive condition derived from a limitation on food resources by external factors. Energy homeostasis is maintained under this condition by using sources other than glucose via adaptive mechanisms. After refeeding, when food is available, other adaptive processes are linked to energy balance. However, less has been reported about the physiological mechanisms present as a result of these conditions, considering the rat as a supraorganism. Metabolic profiling using 1H nuclear magnetic resonance spectroscopy was used to characterize the physiological metabolic differences in urine specimens collected under starved, refed, and recovered conditions. In addition, because starvation induced lack of faecal production and not all animals produced faeces during refeeding, 24 h pooled faecal water samples were also analyzed. Urinary metabolites upregulated by starvation included 2-butanamidoacetate, 3-hydroxyisovalerate, ketoleucine, methylmalonate, p-cresyl glucuronide, p-cresyl sulfate, phenylacetylglycine, pseudouridine, creatinine, taurine, and N-acetyl glycoprotein, which were related to renal and skeletal muscle function, β-oxidation, turnover of proteins and RNA, and host-microbial interactions. Food-derived metabolites, including gut microbial cometabolites, and tricarboxylic acid cycle intermediates were upregulated under refed and recovered conditions, which characterized anabolic urinary metabotypes. The upregulation of creatine and pantothenate indicated an absorptive state after refeeding. Fecal short chain fatty acids, 3-(3-hydroxyphenyl)propionate, lactate, and acetoin provided additional information about the combinatorial metabolism between the host and gut microbiota. This investigation contributes to allow a deeper understanding of physiological responses associated with starvation and refeeding.

AB - © 2016 American Chemical Society. Starvation is a postabsorptive condition derived from a limitation on food resources by external factors. Energy homeostasis is maintained under this condition by using sources other than glucose via adaptive mechanisms. After refeeding, when food is available, other adaptive processes are linked to energy balance. However, less has been reported about the physiological mechanisms present as a result of these conditions, considering the rat as a supraorganism. Metabolic profiling using 1H nuclear magnetic resonance spectroscopy was used to characterize the physiological metabolic differences in urine specimens collected under starved, refed, and recovered conditions. In addition, because starvation induced lack of faecal production and not all animals produced faeces during refeeding, 24 h pooled faecal water samples were also analyzed. Urinary metabolites upregulated by starvation included 2-butanamidoacetate, 3-hydroxyisovalerate, ketoleucine, methylmalonate, p-cresyl glucuronide, p-cresyl sulfate, phenylacetylglycine, pseudouridine, creatinine, taurine, and N-acetyl glycoprotein, which were related to renal and skeletal muscle function, β-oxidation, turnover of proteins and RNA, and host-microbial interactions. Food-derived metabolites, including gut microbial cometabolites, and tricarboxylic acid cycle intermediates were upregulated under refed and recovered conditions, which characterized anabolic urinary metabotypes. The upregulation of creatine and pantothenate indicated an absorptive state after refeeding. Fecal short chain fatty acids, 3-(3-hydroxyphenyl)propionate, lactate, and acetoin provided additional information about the combinatorial metabolism between the host and gut microbiota. This investigation contributes to allow a deeper understanding of physiological responses associated with starvation and refeeding.

UR - https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84985894824&origin=inward

UR - https://www.scopus.com/inward/citedby.uri?partnerID=HzOxMe3b&scp=84985894824&origin=inward

U2 - 10.1021/acs.jproteome.6b00433

DO - 10.1021/acs.jproteome.6b00433

M3 - Article

SP - 3241

EP - 3254

JO - Journal of Proteome Research

JF - Journal of Proteome Research

SN - 1535-3893

ER -