Nitrogen-Fixing and Uricolytic Bacteria Associated with the Gut of Dendroctonus rhizophagus and Dendroctonus valens (Curculionidae: Scolytinae)

Jesús Morales-Jiménez, Arturo Vera-Ponce de León, Aidé García-Domínguez, Esperanza Martínez-Romero, Gerardo Zúñiga, César Hernández-Rodríguez

Research output: Contribution to journalArticle

55 Scopus citations

Abstract

The bark beetles of the genus Dendroctonus feed on phloem that is a nitrogen-limited source. Nitrogen fixation and nitrogen recycling may compensate or alleviate such a limitation, and beetle-associated bacteria capable of such processes were identified. Raoultella terrigena, a diazotrophic bacteria present in the gut of Dendroctonus rhizophagus and D. valens, exhibited high acetylene reduction activity in vitro with different carbon sources, and its nifH and nifD genes were sequenced. Bacteria able to recycle uric acid were Pseudomonas fluorescens DVL3A that used it as carbon and nitrogen source, Serratia proteomaculans 2A CDF and Rahnella aquatilis 6-DR that used uric acid as sole nitrogen source. Also, this is the first report about the uric acid content in whole eggs, larvae, and adults (male and female) samples of the red turpentine beetle (Dendroctonus valens). Our results suggest that the gut bacteria of these bark beetles could contribute to insect N balance. © 2013 Springer Science+Business Media New York.
Original languageAmerican English
Pages (from-to)200-210
Number of pages178
JournalMicrobial Ecology
DOIs
StatePublished - 1 Jul 2013

    Fingerprint

Cite this