Nitrogen deficiency stimulates biosynthesis of bioactive phenylethanoid glycosides in the medicinal plant Castilleja tenuiflora Benth.

Virginia Medina-Pérez, Alma R. López-Laredo, Gabriela Sepúlveda-Jiménez, Alejandro Zamilpa, Gabriela Trejo-Tapia

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

© Franciszek Górski Institute of Plant Physiology, Polish Academy of Sciences, Kraków 2015. Castilleja tenuiflora Benth. (Orobanchaceae) in vitro cultures are alternative sources of phenylethanoid glycosides (PhGs), promising natural products for chronic diseases treatment because of their extensive range of biological activities. To increase the yields of these bioactive compounds, the identification of factors affecting their biosynthesis is required. Here, we show that N deficiency stimulates PhGs biosynthesis by increasing the activity of PAL. The contents of two PhGs, verbascoside and isoverbascoside, were enhanced under N deficiency compared with control conditions. The maximum PAL activity in shoots cultured under N deficiency was also increased. Furthermore, we found de novo anthocyanin synthesis under N deficiency suggesting an additional impact of this stress factor on flavonoid metabolism. N deficiency negatively affected the shoot growth (length, biomass, and total chlorophyll content) and multiplication rate, and inhibited the root formation of C. tenuiflora shoots. Our results demonstrate that N deficiency increases PhGs biosynthesis in C. tenuiflora.
Original languageAmerican English
Pages (from-to)1-8
Number of pages8
JournalActa Physiologiae Plantarum
DOIs
StatePublished - 7 Apr 2015

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Orobanchaceae
Castilleja
Medicinal Plants
Glycosides
medicinal plants
glycosides
Nitrogen
biosynthesis
nitrogen
shoots
Plant Physiological Phenomena
verbascoside
Anthocyanins
plant physiology
Chlorophyll
Biological Products
chronic diseases
Flavonoids
in vitro culture
Biomass

Cite this

@article{11cafd4258d742bfb621d02b56432d81,
title = "Nitrogen deficiency stimulates biosynthesis of bioactive phenylethanoid glycosides in the medicinal plant Castilleja tenuiflora Benth.",
abstract = "{\circledC} Franciszek G{\'o}rski Institute of Plant Physiology, Polish Academy of Sciences, Krak{\'o}w 2015. Castilleja tenuiflora Benth. (Orobanchaceae) in vitro cultures are alternative sources of phenylethanoid glycosides (PhGs), promising natural products for chronic diseases treatment because of their extensive range of biological activities. To increase the yields of these bioactive compounds, the identification of factors affecting their biosynthesis is required. Here, we show that N deficiency stimulates PhGs biosynthesis by increasing the activity of PAL. The contents of two PhGs, verbascoside and isoverbascoside, were enhanced under N deficiency compared with control conditions. The maximum PAL activity in shoots cultured under N deficiency was also increased. Furthermore, we found de novo anthocyanin synthesis under N deficiency suggesting an additional impact of this stress factor on flavonoid metabolism. N deficiency negatively affected the shoot growth (length, biomass, and total chlorophyll content) and multiplication rate, and inhibited the root formation of C. tenuiflora shoots. Our results demonstrate that N deficiency increases PhGs biosynthesis in C. tenuiflora.",
author = "Virginia Medina-P{\'e}rez and L{\'o}pez-Laredo, {Alma R.} and Gabriela Sep{\'u}lveda-Jim{\'e}nez and Alejandro Zamilpa and Gabriela Trejo-Tapia",
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doi = "10.1007/s11738-015-1841-8",
language = "American English",
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Nitrogen deficiency stimulates biosynthesis of bioactive phenylethanoid glycosides in the medicinal plant Castilleja tenuiflora Benth. / Medina-Pérez, Virginia; López-Laredo, Alma R.; Sepúlveda-Jiménez, Gabriela; Zamilpa, Alejandro; Trejo-Tapia, Gabriela.

In: Acta Physiologiae Plantarum, 07.04.2015, p. 1-8.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Nitrogen deficiency stimulates biosynthesis of bioactive phenylethanoid glycosides in the medicinal plant Castilleja tenuiflora Benth.

AU - Medina-Pérez, Virginia

AU - López-Laredo, Alma R.

AU - Sepúlveda-Jiménez, Gabriela

AU - Zamilpa, Alejandro

AU - Trejo-Tapia, Gabriela

PY - 2015/4/7

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N2 - © Franciszek Górski Institute of Plant Physiology, Polish Academy of Sciences, Kraków 2015. Castilleja tenuiflora Benth. (Orobanchaceae) in vitro cultures are alternative sources of phenylethanoid glycosides (PhGs), promising natural products for chronic diseases treatment because of their extensive range of biological activities. To increase the yields of these bioactive compounds, the identification of factors affecting their biosynthesis is required. Here, we show that N deficiency stimulates PhGs biosynthesis by increasing the activity of PAL. The contents of two PhGs, verbascoside and isoverbascoside, were enhanced under N deficiency compared with control conditions. The maximum PAL activity in shoots cultured under N deficiency was also increased. Furthermore, we found de novo anthocyanin synthesis under N deficiency suggesting an additional impact of this stress factor on flavonoid metabolism. N deficiency negatively affected the shoot growth (length, biomass, and total chlorophyll content) and multiplication rate, and inhibited the root formation of C. tenuiflora shoots. Our results demonstrate that N deficiency increases PhGs biosynthesis in C. tenuiflora.

AB - © Franciszek Górski Institute of Plant Physiology, Polish Academy of Sciences, Kraków 2015. Castilleja tenuiflora Benth. (Orobanchaceae) in vitro cultures are alternative sources of phenylethanoid glycosides (PhGs), promising natural products for chronic diseases treatment because of their extensive range of biological activities. To increase the yields of these bioactive compounds, the identification of factors affecting their biosynthesis is required. Here, we show that N deficiency stimulates PhGs biosynthesis by increasing the activity of PAL. The contents of two PhGs, verbascoside and isoverbascoside, were enhanced under N deficiency compared with control conditions. The maximum PAL activity in shoots cultured under N deficiency was also increased. Furthermore, we found de novo anthocyanin synthesis under N deficiency suggesting an additional impact of this stress factor on flavonoid metabolism. N deficiency negatively affected the shoot growth (length, biomass, and total chlorophyll content) and multiplication rate, and inhibited the root formation of C. tenuiflora shoots. Our results demonstrate that N deficiency increases PhGs biosynthesis in C. tenuiflora.

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