TY - JOUR
T1 - Nitrogen deficiency stimulates biosynthesis of bioactive phenylethanoid glycosides in the medicinal plant Castilleja tenuiflora Benth.
AU - Medina-Pérez, Virginia
AU - López-Laredo, Alma R.
AU - Sepúlveda-Jiménez, Gabriela
AU - Zamilpa, Alejandro
AU - Trejo-Tapia, Gabriela
N1 - Publisher Copyright:
© Franciszek Górski Institute of Plant Physiology, Polish Academy of Sciences, Kraków 2015.
PY - 2015/4/7
Y1 - 2015/4/7
N2 - Castilleja tenuiflora Benth. (Orobanchaceae) in vitro cultures are alternative sources of phenylethanoid glycosides (PhGs), promising natural products for chronic diseases treatment because of their extensive range of biological activities. To increase the yields of these bioactive compounds, the identification of factors affecting their biosynthesis is required. Here, we show that N deficiency stimulates PhGs biosynthesis by increasing the activity of PAL. The contents of two PhGs, verbascoside and isoverbascoside, were enhanced under N deficiency compared with control conditions. The maximum PAL activity in shoots cultured under N deficiency was also increased. Furthermore, we found de novo anthocyanin synthesis under N deficiency suggesting an additional impact of this stress factor on flavonoid metabolism. N deficiency negatively affected the shoot growth (length, biomass, and total chlorophyll content) and multiplication rate, and inhibited the root formation of C. tenuiflora shoots. Our results demonstrate that N deficiency increases PhGs biosynthesis in C. tenuiflora.
AB - Castilleja tenuiflora Benth. (Orobanchaceae) in vitro cultures are alternative sources of phenylethanoid glycosides (PhGs), promising natural products for chronic diseases treatment because of their extensive range of biological activities. To increase the yields of these bioactive compounds, the identification of factors affecting their biosynthesis is required. Here, we show that N deficiency stimulates PhGs biosynthesis by increasing the activity of PAL. The contents of two PhGs, verbascoside and isoverbascoside, were enhanced under N deficiency compared with control conditions. The maximum PAL activity in shoots cultured under N deficiency was also increased. Furthermore, we found de novo anthocyanin synthesis under N deficiency suggesting an additional impact of this stress factor on flavonoid metabolism. N deficiency negatively affected the shoot growth (length, biomass, and total chlorophyll content) and multiplication rate, and inhibited the root formation of C. tenuiflora shoots. Our results demonstrate that N deficiency increases PhGs biosynthesis in C. tenuiflora.
KW - Acteoside
KW - Anthocyanins
KW - Castilleja tenuiflora
KW - Nitrogen
KW - PAL
KW - Temporary immersion system
KW - Verbascoside
UR - http://www.scopus.com/inward/record.url?scp=84928335010&partnerID=8YFLogxK
U2 - 10.1007/s11738-015-1841-8
DO - 10.1007/s11738-015-1841-8
M3 - Artículo
SN - 0137-5881
VL - 37
SP - 1
EP - 8
JO - Acta Physiologiae Plantarum
JF - Acta Physiologiae Plantarum
IS - 5
M1 - 93
ER -