TY - JOUR
T1 - Neutrophil extracellular traps and MPO in models of susceptibility and resistance against Entamoeba histolytica
AU - Contis Montes de Oca, Arturo
AU - Cruz Baquero, Andrea
AU - Campos Rodríguez, Rafael
AU - Cárdenas Jaramillo, Luz María
AU - Aguayo Flores, José Eduardo
AU - Rojas Hernández, Saúl
AU - Olivos García, Alfonso
AU - Pacheco Yepez, Judith
N1 - Publisher Copyright:
© 2020 John Wiley & Sons Ltd
PY - 2020/6/1
Y1 - 2020/6/1
N2 - The main effector mechanisms of neutrophils are the release of neutrophil extracellular traps (NETs) and myeloperoxidase (MPO). In this work, we evaluated the role of NETs and the activity of MPO in the interactions of rodent neutrophils with amoebae and in amoebic liver abscess (ALA)-resistant and ALA-susceptible models. We showed with in vitro assays that mice produced greater amounts of NETs and MPO than did hamsters, and the elastase activity was high in both models. However, the inhibition of NETs and MPO promoted an increase in amoeba viability in the mice. The mouse ALAs showed a more profound presence of NETs and MPO than did the hamster ALAs. We concluded that both effector mechanisms were essential for the amoebic damage and could prevent the formation of ALAs in the resistant model.
AB - The main effector mechanisms of neutrophils are the release of neutrophil extracellular traps (NETs) and myeloperoxidase (MPO). In this work, we evaluated the role of NETs and the activity of MPO in the interactions of rodent neutrophils with amoebae and in amoebic liver abscess (ALA)-resistant and ALA-susceptible models. We showed with in vitro assays that mice produced greater amounts of NETs and MPO than did hamsters, and the elastase activity was high in both models. However, the inhibition of NETs and MPO promoted an increase in amoeba viability in the mice. The mouse ALAs showed a more profound presence of NETs and MPO than did the hamster ALAs. We concluded that both effector mechanisms were essential for the amoebic damage and could prevent the formation of ALAs in the resistant model.
KW - BALB/c mice
KW - Entamoeba histolytica
KW - amoebic liver abscess
KW - hamster
KW - myeloperoxidase
KW - neutrophils extracellular traps
UR - http://www.scopus.com/inward/record.url?scp=85082921871&partnerID=8YFLogxK
U2 - 10.1111/pim.12714
DO - 10.1111/pim.12714
M3 - Artículo
C2 - 32187688
AN - SCOPUS:85082921871
SN - 0141-9838
VL - 42
JO - Parasite Immunology
JF - Parasite Immunology
IS - 6
M1 - e12714
ER -