TY - JOUR
T1 - Mycobacterium tuberculosis H37Rv induces ectosome release in human polymorphonuclear neutrophils
AU - González-Cano, Patricia
AU - Mondragón-Flores, Ricardo
AU - Sánchez-Torres, Luvia E.
AU - González-Pozos, Sirenia
AU - Silva-Miranda, Mayra
AU - Monroy-Ostria, Amalia
AU - Estrada-Parra, Sergio
AU - Estrada-García, Iris
N1 - Funding Information:
This work was supported by CONACYT Grant No. 42512 and SIP 20091338.
PY - 2010/3
Y1 - 2010/3
N2 - Ectocytosis, the cellular process by which ectosomes (Ects) are released, is an important phenomenon by which eukaryotic cells exchange molecular information. Ects released from N-formylmethionyl-leucyl-phenylalanine (fMLP)-activated human polymorphonuclear neutrophils (PMNs) have recently been characterized. Molecules such as CD35 and phosphatidylserine (PS), and enzymes such as myeloperoxidase and elastase were found in these vesicles, suggesting that Ects from PMNs could function as ecto-organelles with anti-microbial activity. Here we show for the first time that human PMNs release ectosomes in response to Mycobacterium tuberculosis H37Rv infection. We found that the release of ectosomes was not associated exclusively with mycobacterial infection since infection with other microorganisms (e.g., Leishmania mexicana, Staphylococcus aureus, and Escherichia coli or activation with phorbol myristate acetate (PMA)) also induced ectocytosis. Ects release started as early as 10 min after infection or activation. Expression of CD35, PS, Rab5, Rab7 and gp91Phox, a subunit of Cyt b555 was demonstrated on the Ects membrane. Based on our observations we conclude that Ects are released from human neutrophils in response to cell activation and that this process is not related to apoptosis.
AB - Ectocytosis, the cellular process by which ectosomes (Ects) are released, is an important phenomenon by which eukaryotic cells exchange molecular information. Ects released from N-formylmethionyl-leucyl-phenylalanine (fMLP)-activated human polymorphonuclear neutrophils (PMNs) have recently been characterized. Molecules such as CD35 and phosphatidylserine (PS), and enzymes such as myeloperoxidase and elastase were found in these vesicles, suggesting that Ects from PMNs could function as ecto-organelles with anti-microbial activity. Here we show for the first time that human PMNs release ectosomes in response to Mycobacterium tuberculosis H37Rv infection. We found that the release of ectosomes was not associated exclusively with mycobacterial infection since infection with other microorganisms (e.g., Leishmania mexicana, Staphylococcus aureus, and Escherichia coli or activation with phorbol myristate acetate (PMA)) also induced ectocytosis. Ects release started as early as 10 min after infection or activation. Expression of CD35, PS, Rab5, Rab7 and gp91Phox, a subunit of Cyt b555 was demonstrated on the Ects membrane. Based on our observations we conclude that Ects are released from human neutrophils in response to cell activation and that this process is not related to apoptosis.
KW - NADPH oxidase
KW - Neutrophils
KW - Vesicles
UR - http://www.scopus.com/inward/record.url?scp=77950594307&partnerID=8YFLogxK
U2 - 10.1016/j.tube.2010.01.002
DO - 10.1016/j.tube.2010.01.002
M3 - Artículo
SN - 1472-9792
VL - 90
SP - 125
EP - 134
JO - Tuberculosis
JF - Tuberculosis
IS - 2
ER -