Muscarinic receptors control markers of inflammation in the small intestine of BALB/c mice

Ivonne Maciel Arciniega-Martínez; Judith Pacheco-Yépez; Mónica Miguel Santamaria-Chávez; Xóchitl Abril Rebollar-Ruíz; Luz María Cárdenas-Jaramillo; Rosa Adriana Jarillo-Luna; Rafael Campos-Rodríguez; Maria Elisa Drago-Serrano; Aldo Arturo Reséndiz-Albor

doi:10.1016/j.jneuroim.2021.577764

Muscarinic receptors control markers of inflammation in the small intestine of BALB/c mice

Ivonne Maciel Arciniega-Martínez, Judith Pacheco-Yépez, Mónica Miguel Santamaria-Chávez, Xóchitl Abril Rebollar-Ruíz, Luz María Cárdenas-Jaramillo, Rosa Adriana Jarillo-Luna, Rafael Campos-Rodríguez, Maria Elisa Drago-Serrano, Aldo Arturo Reséndiz-Albor

Escuela Superior de Medicina (ESM)

Research output: Contribution to journal › Article › peer-review

Abstract

Muscarinic-acetylcholine-receptors (mAChRs) modulate intestinal homeostasis, but their role in inflammation is unclear; thus, this issue was the focus of this study. BALB/c mice were treated for 7 days with muscarine (mAChR/agonist), atropine (mAChR/antagonist) or saline. Small-intestine samples were collected for histology and cytofluorometric assays in Peyer's patches (PP) and lamina propria (LP) cell-suspensions. In LP, goblet-cells/leukocytes/neutrophils/MPO⁺ cells and MPO/activity were increased in the muscarine group. In PP, IFN-γ⁺/CD4⁺ T or IL-6⁺/CD4⁺ T cell numbers were higher in the muscarine or atropine groups, respectively. In LP, TNF-α⁺/CD4⁺ T cell number was higher in the muscarine group and lower in the atropine.

Original language	English
Article number	577764
Journal	Journal of Neuroimmunology
Volume	362
DOIs	https://doi.org/10.1016/j.jneuroim.2021.577764
State	Published - 15 Jan 2022

Keywords

Cholinergic antagonists
Goblet cells
Intestinal inflammation
Muscarinic receptors
Myeloperoxidase
Neutrophils

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10.1016/j.jneuroim.2021.577764

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Arciniega-Martínez, I. M., Pacheco-Yépez, J., Santamaria-Chávez, M. M., Rebollar-Ruíz, X. A., Cárdenas-Jaramillo, L. M., Jarillo-Luna, R. A., Campos-Rodríguez, R., Drago-Serrano, M. E., & Reséndiz-Albor, A. A. (2022). Muscarinic receptors control markers of inflammation in the small intestine of BALB/c mice. Journal of Neuroimmunology, 362, Article 577764. https://doi.org/10.1016/j.jneuroim.2021.577764

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title = "Muscarinic receptors control markers of inflammation in the small intestine of BALB/c mice",

abstract = "Muscarinic-acetylcholine-receptors (mAChRs) modulate intestinal homeostasis, but their role in inflammation is unclear; thus, this issue was the focus of this study. BALB/c mice were treated for 7 days with muscarine (mAChR/agonist), atropine (mAChR/antagonist) or saline. Small-intestine samples were collected for histology and cytofluorometric assays in Peyer's patches (PP) and lamina propria (LP) cell-suspensions. In LP, goblet-cells/leukocytes/neutrophils/MPO+ cells and MPO/activity were increased in the muscarine group. In PP, IFN-γ+/CD4+ T or IL-6+/CD4+ T cell numbers were higher in the muscarine or atropine groups, respectively. In LP, TNF-α+/CD4+ T cell number was higher in the muscarine group and lower in the atropine.",

keywords = "Cholinergic antagonists, Goblet cells, Intestinal inflammation, Muscarinic receptors, Myeloperoxidase, Neutrophils",

author = "Arciniega-Mart{\'i}nez, {Ivonne Maciel} and Judith Pacheco-Y{\'e}pez and Santamaria-Ch{\'a}vez, {M{\'o}nica Miguel} and Rebollar-Ru{\'i}z, {X{\'o}chitl Abril} and C{\'a}rdenas-Jaramillo, {Luz Mar{\'i}a} and Jarillo-Luna, {Rosa Adriana} and Rafael Campos-Rodr{\'i}guez and Drago-Serrano, {Maria Elisa} and Res{\'e}ndiz-Albor, {Aldo Arturo}",

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Arciniega-Martínez, IM , Pacheco-Yépez, J, Santamaria-Chávez, MM, Rebollar-Ruíz, XA, Cárdenas-Jaramillo, LM, Jarillo-Luna, RA, Campos-Rodríguez, R, Drago-Serrano, ME & Reséndiz-Albor, AA 2022, 'Muscarinic receptors control markers of inflammation in the small intestine of BALB/c mice', Journal of Neuroimmunology, vol. 362, 577764. https://doi.org/10.1016/j.jneuroim.2021.577764

TY - JOUR

T1 - Muscarinic receptors control markers of inflammation in the small intestine of BALB/c mice

AU - Arciniega-Martínez, Ivonne Maciel

AU - Pacheco-Yépez, Judith

AU - Santamaria-Chávez, Mónica Miguel

AU - Rebollar-Ruíz, Xóchitl Abril

AU - Cárdenas-Jaramillo, Luz María

AU - Jarillo-Luna, Rosa Adriana

AU - Campos-Rodríguez, Rafael

AU - Drago-Serrano, Maria Elisa

AU - Reséndiz-Albor, Aldo Arturo

PY - 2022/1/15

Y1 - 2022/1/15

N2 - Muscarinic-acetylcholine-receptors (mAChRs) modulate intestinal homeostasis, but their role in inflammation is unclear; thus, this issue was the focus of this study. BALB/c mice were treated for 7 days with muscarine (mAChR/agonist), atropine (mAChR/antagonist) or saline. Small-intestine samples were collected for histology and cytofluorometric assays in Peyer's patches (PP) and lamina propria (LP) cell-suspensions. In LP, goblet-cells/leukocytes/neutrophils/MPO+ cells and MPO/activity were increased in the muscarine group. In PP, IFN-γ+/CD4+ T or IL-6+/CD4+ T cell numbers were higher in the muscarine or atropine groups, respectively. In LP, TNF-α+/CD4+ T cell number was higher in the muscarine group and lower in the atropine.

AB - Muscarinic-acetylcholine-receptors (mAChRs) modulate intestinal homeostasis, but their role in inflammation is unclear; thus, this issue was the focus of this study. BALB/c mice were treated for 7 days with muscarine (mAChR/agonist), atropine (mAChR/antagonist) or saline. Small-intestine samples were collected for histology and cytofluorometric assays in Peyer's patches (PP) and lamina propria (LP) cell-suspensions. In LP, goblet-cells/leukocytes/neutrophils/MPO+ cells and MPO/activity were increased in the muscarine group. In PP, IFN-γ+/CD4+ T or IL-6+/CD4+ T cell numbers were higher in the muscarine or atropine groups, respectively. In LP, TNF-α+/CD4+ T cell number was higher in the muscarine group and lower in the atropine.

KW - Cholinergic antagonists

KW - Goblet cells

KW - Intestinal inflammation

KW - Muscarinic receptors

KW - Myeloperoxidase

KW - Neutrophils

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U2 - 10.1016/j.jneuroim.2021.577764

DO - 10.1016/j.jneuroim.2021.577764

M3 - Artículo

C2 - 34823118

AN - SCOPUS:85119409813

SN - 0165-5728

VL - 362

JO - Journal of Neuroimmunology

JF - Journal of Neuroimmunology

M1 - 577764

ER -

Muscarinic receptors control markers of inflammation in the small intestine of BALB/c mice

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Keywords

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