Multiphasic characterization of a plant growth promoting bacterial strain, Burkholderia sp. 7016 and its effect on tomato growth in the field

Miao Gao, Jian Jiao Zhou, En Tao Wang, Qian Chen, Jing Xu, Jian Guang Sun

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

© 2015 Chinese Academy of Agricultural Sciences. Aiming at searching for plant growth promoting rhizobacteria (PGPR), a bacterium strain coded as 7016 was isolated from soybean rhizosphere and was characterized in the present study. It was identified as Burkholderia sp. based on 16S rDNA sequence analysis, as well as phenotypic and biochemical characterizations. This bacterium presented nitrogenase activity, 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase activity and phosphate solubilizing ability; inhibited the growth of Sclerotinia sclerotiorum, Gibberella zeae and Verticillium dahliae; and produced small quantities of indole acetic acid (IAA). In green house experiments, significant increases in shoot height and weight, root length and weight, and stem diameter were observed on tomato plants in 30 d after inoculation with strain 7016. Result of 16S rDNA PCR-DGGE showed that 7016 survived in the rhizosphere of tomato seedlings. In the field experiments, Burkholderia sp. 7016 enhanced the tomato yield and significantly promoted activities of soil urease, phosphatase, sucrase, and catalase. All these results demonstrated Burkholderia sp. 7016 as a valuable PGPR and a candidate of biofertilizer.
Original languageAmerican English
Pages (from-to)1855-1863
Number of pages1668
JournalJournal of Integrative Agriculture
DOIs
StatePublished - 1 Jan 2015

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Burkholderia
Lycopersicon esculentum
Rhizosphere
plant growth-promoting rhizobacteria
plant growth
tomatoes
rhizobacterium
Ribosomal DNA
rhizosphere
Bacteria
Growth
Gibberella
Verticillium
1-aminocyclopropane-1-carboxylate deaminase
Sucrase
agricultural science
biofertilizer
Weights and Measures
Nitrogenase
Ascomycota

Cite this

Gao, Miao ; Zhou, Jian Jiao ; Wang, En Tao ; Chen, Qian ; Xu, Jing ; Sun, Jian Guang. / Multiphasic characterization of a plant growth promoting bacterial strain, Burkholderia sp. 7016 and its effect on tomato growth in the field. In: Journal of Integrative Agriculture. 2015 ; pp. 1855-1863.
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Multiphasic characterization of a plant growth promoting bacterial strain, Burkholderia sp. 7016 and its effect on tomato growth in the field. / Gao, Miao; Zhou, Jian Jiao; Wang, En Tao; Chen, Qian; Xu, Jing; Sun, Jian Guang.

In: Journal of Integrative Agriculture, 01.01.2015, p. 1855-1863.

Research output: Contribution to journalArticle

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