Multidrug- and extensively drug-resistant uropathogenic Escherichia coli clinical strains: Phylogenetic groups widely associated with integrons maintain high genetic diversity

Sara A. Ochoa, Ariadnna Cruz-Córdova, Victor M. Luna-Pineda, Juan P. Reyes-Grajeda, Vicenta Cázares-Domínguez, Gerardo Escalona, Ma Eugenia Sepúlveda-González, Fernanda López-Montiel, José Arellano-Galindo, Briceida López-Martínez, Israel Parra-Ortega, Silvia Giono-Cerezo, Rigoberto Hernández-Castro, Daniela de la Rosa-Zamboni, Juan Xicohtencatl-Cortes

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Abstract

© 2016 Ochoa, Cruz-Córdova, Luna-Pineda, Reyes-Grajeda, Cázares-Domínguez, Escalona, Sepúlveda-González, López-Montiel, Arellano-Galindo, López-Martínez, Parra-Ortega, Giono-Cerezo, Hernández-Castro, de la Rosa-Zamboni and Xicohtencatl-Cortes. In recent years, an increase of uropathogenic Escherichia coli (UPEC) strains with Multidrug-resistant (MDR) and Extensively Drug-resistant (XDR) profiles that complicate therapy for urinary tract infections (UTIs) has been observed and has directly impacted costs and extended hospital stays. The aim of this study was to determine MDR- and XDR-UPEC clinical strains, their virulence genes, their phylogenetic groups and to ascertain their relationship with integrons and genetic diversity. From a collection of 500 UPEC strains, 103 were selected with MDR and XDR characteristics. MDR-UPEC strains were mainly associated with phylogenetic groups D (54.87%) and B2 (39.02%) with a high percentage (≥70%) of several fimbrial genes (ecpA, fimH, csgA, and papGII), an iron uptake gene (chuA), and a toxin gene (hlyA). In addition, a moderate frequency (40-70%) of other genes (iutD, tosA, and bcsA) was observed. XDR-UPEC strains were predominantly associated with phylogenetic groups B2 (47.61%) and D (42.85%), which grouped with ≥80 virulence genes, including ecpA, fimH, csgA, papGII, iutD, and chuA. A moderate frequency (40-70%) of the tosA and hlyA genes was observed. The class 1 and 2 integrons that were identified in the MDR- and XDR-UPEC strains were associated with phylogenetic groups D, B2, and A, while the XDR-UPEC strains that were associated with phylogenetic groups B2, D, and A showed an extended-spectrum beta-lactamase (ESBL) phenotype. The modifying enzymes (aadA1, aadB, aacC, ant1, dfrA1, dfrA17, and aadA4) that were identified in the variable region of class 1 and 2 integrons from the MDR strains showed resistance to gentamycin (56.25 and 66.66%, respectively) and trimethoprim-sulfamethoxazole (84.61 and 66.66%, respectively). The MDR- and XDR-UPEC strains were distributed into seven clusters and were closely related to phylogenic groups B2 and D. The diversity analysis by PFGE showed 42.68% of clones of MDR-UPEC and no clonal association in the XDR-UPEC strains. In conclusion, phylogenetic groups including virulence genes are widely associated with two integron classes (1 and 2) in MDR- and XDR-UPEC strains.
Original languageAmerican English
JournalFrontiers in Microbiology
DOIs
StatePublished - 1 Jan 2016

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Uropathogenic Escherichia coli
Integrons
Pharmaceutical Preparations
Genes
Virulence
Sulfamethoxazole Drug Combination Trimethoprim
beta-Lactamases
Gentamicins
Urinary Tract Infections
Length of Stay

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Ochoa, Sara A. ; Cruz-Córdova, Ariadnna ; Luna-Pineda, Victor M. ; Reyes-Grajeda, Juan P. ; Cázares-Domínguez, Vicenta ; Escalona, Gerardo ; Sepúlveda-González, Ma Eugenia ; López-Montiel, Fernanda ; Arellano-Galindo, José ; López-Martínez, Briceida ; Parra-Ortega, Israel ; Giono-Cerezo, Silvia ; Hernández-Castro, Rigoberto ; de la Rosa-Zamboni, Daniela ; Xicohtencatl-Cortes, Juan. / Multidrug- and extensively drug-resistant uropathogenic Escherichia coli clinical strains: Phylogenetic groups widely associated with integrons maintain high genetic diversity. In: Frontiers in Microbiology. 2016.
@article{a4b9bccfd4c64b4ba97abbedc1d21557,
title = "Multidrug- and extensively drug-resistant uropathogenic Escherichia coli clinical strains: Phylogenetic groups widely associated with integrons maintain high genetic diversity",
abstract = "{\circledC} 2016 Ochoa, Cruz-C{\'o}rdova, Luna-Pineda, Reyes-Grajeda, C{\'a}zares-Dom{\'i}nguez, Escalona, Sep{\'u}lveda-Gonz{\'a}lez, L{\'o}pez-Montiel, Arellano-Galindo, L{\'o}pez-Mart{\'i}nez, Parra-Ortega, Giono-Cerezo, Hern{\'a}ndez-Castro, de la Rosa-Zamboni and Xicohtencatl-Cortes. In recent years, an increase of uropathogenic Escherichia coli (UPEC) strains with Multidrug-resistant (MDR) and Extensively Drug-resistant (XDR) profiles that complicate therapy for urinary tract infections (UTIs) has been observed and has directly impacted costs and extended hospital stays. The aim of this study was to determine MDR- and XDR-UPEC clinical strains, their virulence genes, their phylogenetic groups and to ascertain their relationship with integrons and genetic diversity. From a collection of 500 UPEC strains, 103 were selected with MDR and XDR characteristics. MDR-UPEC strains were mainly associated with phylogenetic groups D (54.87{\%}) and B2 (39.02{\%}) with a high percentage (≥70{\%}) of several fimbrial genes (ecpA, fimH, csgA, and papGII), an iron uptake gene (chuA), and a toxin gene (hlyA). In addition, a moderate frequency (40-70{\%}) of other genes (iutD, tosA, and bcsA) was observed. XDR-UPEC strains were predominantly associated with phylogenetic groups B2 (47.61{\%}) and D (42.85{\%}), which grouped with ≥80 virulence genes, including ecpA, fimH, csgA, papGII, iutD, and chuA. A moderate frequency (40-70{\%}) of the tosA and hlyA genes was observed. The class 1 and 2 integrons that were identified in the MDR- and XDR-UPEC strains were associated with phylogenetic groups D, B2, and A, while the XDR-UPEC strains that were associated with phylogenetic groups B2, D, and A showed an extended-spectrum beta-lactamase (ESBL) phenotype. The modifying enzymes (aadA1, aadB, aacC, ant1, dfrA1, dfrA17, and aadA4) that were identified in the variable region of class 1 and 2 integrons from the MDR strains showed resistance to gentamycin (56.25 and 66.66{\%}, respectively) and trimethoprim-sulfamethoxazole (84.61 and 66.66{\%}, respectively). The MDR- and XDR-UPEC strains were distributed into seven clusters and were closely related to phylogenic groups B2 and D. The diversity analysis by PFGE showed 42.68{\%} of clones of MDR-UPEC and no clonal association in the XDR-UPEC strains. In conclusion, phylogenetic groups including virulence genes are widely associated with two integron classes (1 and 2) in MDR- and XDR-UPEC strains.",
author = "Ochoa, {Sara A.} and Ariadnna Cruz-C{\'o}rdova and Luna-Pineda, {Victor M.} and Reyes-Grajeda, {Juan P.} and Vicenta C{\'a}zares-Dom{\'i}nguez and Gerardo Escalona and Sep{\'u}lveda-Gonz{\'a}lez, {Ma Eugenia} and Fernanda L{\'o}pez-Montiel and Jos{\'e} Arellano-Galindo and Briceida L{\'o}pez-Mart{\'i}nez and Israel Parra-Ortega and Silvia Giono-Cerezo and Rigoberto Hern{\'a}ndez-Castro and {de la Rosa-Zamboni}, Daniela and Juan Xicohtencatl-Cortes",
year = "2016",
month = "1",
day = "1",
doi = "10.3389/fmicb.2016.02042",
language = "American English",
journal = "Frontiers in Microbiology",
issn = "1664-302X",
publisher = "Frontiers Media S.A.",

}

Ochoa, SA, Cruz-Córdova, A, Luna-Pineda, VM, Reyes-Grajeda, JP, Cázares-Domínguez, V, Escalona, G, Sepúlveda-González, ME, López-Montiel, F, Arellano-Galindo, J, López-Martínez, B, Parra-Ortega, I, Giono-Cerezo, S, Hernández-Castro, R, de la Rosa-Zamboni, D & Xicohtencatl-Cortes, J 2016, 'Multidrug- and extensively drug-resistant uropathogenic Escherichia coli clinical strains: Phylogenetic groups widely associated with integrons maintain high genetic diversity', Frontiers in Microbiology. https://doi.org/10.3389/fmicb.2016.02042

Multidrug- and extensively drug-resistant uropathogenic Escherichia coli clinical strains: Phylogenetic groups widely associated with integrons maintain high genetic diversity. / Ochoa, Sara A.; Cruz-Córdova, Ariadnna; Luna-Pineda, Victor M.; Reyes-Grajeda, Juan P.; Cázares-Domínguez, Vicenta; Escalona, Gerardo; Sepúlveda-González, Ma Eugenia; López-Montiel, Fernanda; Arellano-Galindo, José; López-Martínez, Briceida; Parra-Ortega, Israel; Giono-Cerezo, Silvia; Hernández-Castro, Rigoberto; de la Rosa-Zamboni, Daniela; Xicohtencatl-Cortes, Juan.

In: Frontiers in Microbiology, 01.01.2016.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Multidrug- and extensively drug-resistant uropathogenic Escherichia coli clinical strains: Phylogenetic groups widely associated with integrons maintain high genetic diversity

AU - Ochoa, Sara A.

AU - Cruz-Córdova, Ariadnna

AU - Luna-Pineda, Victor M.

AU - Reyes-Grajeda, Juan P.

AU - Cázares-Domínguez, Vicenta

AU - Escalona, Gerardo

AU - Sepúlveda-González, Ma Eugenia

AU - López-Montiel, Fernanda

AU - Arellano-Galindo, José

AU - López-Martínez, Briceida

AU - Parra-Ortega, Israel

AU - Giono-Cerezo, Silvia

AU - Hernández-Castro, Rigoberto

AU - de la Rosa-Zamboni, Daniela

AU - Xicohtencatl-Cortes, Juan

PY - 2016/1/1

Y1 - 2016/1/1

N2 - © 2016 Ochoa, Cruz-Córdova, Luna-Pineda, Reyes-Grajeda, Cázares-Domínguez, Escalona, Sepúlveda-González, López-Montiel, Arellano-Galindo, López-Martínez, Parra-Ortega, Giono-Cerezo, Hernández-Castro, de la Rosa-Zamboni and Xicohtencatl-Cortes. In recent years, an increase of uropathogenic Escherichia coli (UPEC) strains with Multidrug-resistant (MDR) and Extensively Drug-resistant (XDR) profiles that complicate therapy for urinary tract infections (UTIs) has been observed and has directly impacted costs and extended hospital stays. The aim of this study was to determine MDR- and XDR-UPEC clinical strains, their virulence genes, their phylogenetic groups and to ascertain their relationship with integrons and genetic diversity. From a collection of 500 UPEC strains, 103 were selected with MDR and XDR characteristics. MDR-UPEC strains were mainly associated with phylogenetic groups D (54.87%) and B2 (39.02%) with a high percentage (≥70%) of several fimbrial genes (ecpA, fimH, csgA, and papGII), an iron uptake gene (chuA), and a toxin gene (hlyA). In addition, a moderate frequency (40-70%) of other genes (iutD, tosA, and bcsA) was observed. XDR-UPEC strains were predominantly associated with phylogenetic groups B2 (47.61%) and D (42.85%), which grouped with ≥80 virulence genes, including ecpA, fimH, csgA, papGII, iutD, and chuA. A moderate frequency (40-70%) of the tosA and hlyA genes was observed. The class 1 and 2 integrons that were identified in the MDR- and XDR-UPEC strains were associated with phylogenetic groups D, B2, and A, while the XDR-UPEC strains that were associated with phylogenetic groups B2, D, and A showed an extended-spectrum beta-lactamase (ESBL) phenotype. The modifying enzymes (aadA1, aadB, aacC, ant1, dfrA1, dfrA17, and aadA4) that were identified in the variable region of class 1 and 2 integrons from the MDR strains showed resistance to gentamycin (56.25 and 66.66%, respectively) and trimethoprim-sulfamethoxazole (84.61 and 66.66%, respectively). The MDR- and XDR-UPEC strains were distributed into seven clusters and were closely related to phylogenic groups B2 and D. The diversity analysis by PFGE showed 42.68% of clones of MDR-UPEC and no clonal association in the XDR-UPEC strains. In conclusion, phylogenetic groups including virulence genes are widely associated with two integron classes (1 and 2) in MDR- and XDR-UPEC strains.

AB - © 2016 Ochoa, Cruz-Córdova, Luna-Pineda, Reyes-Grajeda, Cázares-Domínguez, Escalona, Sepúlveda-González, López-Montiel, Arellano-Galindo, López-Martínez, Parra-Ortega, Giono-Cerezo, Hernández-Castro, de la Rosa-Zamboni and Xicohtencatl-Cortes. In recent years, an increase of uropathogenic Escherichia coli (UPEC) strains with Multidrug-resistant (MDR) and Extensively Drug-resistant (XDR) profiles that complicate therapy for urinary tract infections (UTIs) has been observed and has directly impacted costs and extended hospital stays. The aim of this study was to determine MDR- and XDR-UPEC clinical strains, their virulence genes, their phylogenetic groups and to ascertain their relationship with integrons and genetic diversity. From a collection of 500 UPEC strains, 103 were selected with MDR and XDR characteristics. MDR-UPEC strains were mainly associated with phylogenetic groups D (54.87%) and B2 (39.02%) with a high percentage (≥70%) of several fimbrial genes (ecpA, fimH, csgA, and papGII), an iron uptake gene (chuA), and a toxin gene (hlyA). In addition, a moderate frequency (40-70%) of other genes (iutD, tosA, and bcsA) was observed. XDR-UPEC strains were predominantly associated with phylogenetic groups B2 (47.61%) and D (42.85%), which grouped with ≥80 virulence genes, including ecpA, fimH, csgA, papGII, iutD, and chuA. A moderate frequency (40-70%) of the tosA and hlyA genes was observed. The class 1 and 2 integrons that were identified in the MDR- and XDR-UPEC strains were associated with phylogenetic groups D, B2, and A, while the XDR-UPEC strains that were associated with phylogenetic groups B2, D, and A showed an extended-spectrum beta-lactamase (ESBL) phenotype. The modifying enzymes (aadA1, aadB, aacC, ant1, dfrA1, dfrA17, and aadA4) that were identified in the variable region of class 1 and 2 integrons from the MDR strains showed resistance to gentamycin (56.25 and 66.66%, respectively) and trimethoprim-sulfamethoxazole (84.61 and 66.66%, respectively). The MDR- and XDR-UPEC strains were distributed into seven clusters and were closely related to phylogenic groups B2 and D. The diversity analysis by PFGE showed 42.68% of clones of MDR-UPEC and no clonal association in the XDR-UPEC strains. In conclusion, phylogenetic groups including virulence genes are widely associated with two integron classes (1 and 2) in MDR- and XDR-UPEC strains.

U2 - 10.3389/fmicb.2016.02042

DO - 10.3389/fmicb.2016.02042

M3 - Article

JO - Frontiers in Microbiology

JF - Frontiers in Microbiology

SN - 1664-302X

ER -