Morphology, biochemistry, and growth of raphidophyte strains from the Gulf of California

C. J. Band-Schmidt, A. Martínez-López, J. J. Bustillos-Guzmán, L. Carreón-Palau, L. Morquecho, N. O. Olguín-Monroy, T. Zenteno-Savín, A. Mendoza-Flores, B. González-Acosta, F. H. Hernández-Sandoval, C. Tomas

Research output: Contribution to journalArticle

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Abstract

Strains of raphidophytes from different regions of the Gulf of California were established for identification and biochemical description. Raphidophyte in our coasts have been traditionally identified based on microscopic observations, a biochemical analyses of strains present in our coast is needed for a more detailed characterization and species confirmation. Strains were identified by morphological observations and sequencing of the 5. 8S and COI regions. Cells were cultivated in modified f/2 media at 20°C with a light-dark cycle (12 h:12 h) and 150 μmol m -2 s -1 light intensity. Pigments were identified by HPLC, brevetoxins by LC-MS/MS, fatty acids by gas chromatography, superoxide radicals by spectrophotometry, and lipid peroxidation by the determination of thiobarbituric acid reactive substances. Strains were identified as Chattonella subsalsa, C. marina, and Fibrocapsa japonica. In all strains, the main pigment was chlorophyll a, followed by fucoxanthin, chlorophyll c1 and c2, violaxanthin, β-carotene, and diadinoxanthin. Strains were tested for PbTx-1, PbTx-2, PbTx-3, PbTx-6, PbTx-9, PbTx-carboxylic acid, brevenal, and brevisin; none were detected. All strains presented superoxide radical production and lipid peroxidation. The main fatty acids were 18:4 (n-3) and 20:5 (n-3). Strains had typical fatty acid composition for raphidophytes and produced brevetoxin-like compounds, had superoxide radical production, and lipid peroxidation. With this contribution, we confirm the presence of C. subsalsa, C. marina and F. japonica in the Gulf of California. © 2012 Springer Science+Business Media B.V.
Original languageAmerican English
Pages (from-to)81-97
Number of pages71
JournalHydrobiologia
DOIs
StatePublished - 1 Sep 2012
Externally publishedYes

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biochemistry
Gulf of California
fatty acid
lipid
marina
pigment
coast
brevetoxins
superoxide anion
carboxylic acid
spectrophotometry
light intensity
lipid peroxidation
gas chromatography
chlorophyll a
chlorophyll
acid
pigments
fatty acids
coasts

Cite this

Band-Schmidt, C. J. ; Martínez-López, A. ; Bustillos-Guzmán, J. J. ; Carreón-Palau, L. ; Morquecho, L. ; Olguín-Monroy, N. O. ; Zenteno-Savín, T. ; Mendoza-Flores, A. ; González-Acosta, B. ; Hernández-Sandoval, F. H. ; Tomas, C. / Morphology, biochemistry, and growth of raphidophyte strains from the Gulf of California. In: Hydrobiologia. 2012 ; pp. 81-97.
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abstract = "Strains of raphidophytes from different regions of the Gulf of California were established for identification and biochemical description. Raphidophyte in our coasts have been traditionally identified based on microscopic observations, a biochemical analyses of strains present in our coast is needed for a more detailed characterization and species confirmation. Strains were identified by morphological observations and sequencing of the 5. 8S and COI regions. Cells were cultivated in modified f/2 media at 20°C with a light-dark cycle (12 h:12 h) and 150 μmol m -2 s -1 light intensity. Pigments were identified by HPLC, brevetoxins by LC-MS/MS, fatty acids by gas chromatography, superoxide radicals by spectrophotometry, and lipid peroxidation by the determination of thiobarbituric acid reactive substances. Strains were identified as Chattonella subsalsa, C. marina, and Fibrocapsa japonica. In all strains, the main pigment was chlorophyll a, followed by fucoxanthin, chlorophyll c1 and c2, violaxanthin, β-carotene, and diadinoxanthin. Strains were tested for PbTx-1, PbTx-2, PbTx-3, PbTx-6, PbTx-9, PbTx-carboxylic acid, brevenal, and brevisin; none were detected. All strains presented superoxide radical production and lipid peroxidation. The main fatty acids were 18:4 (n-3) and 20:5 (n-3). Strains had typical fatty acid composition for raphidophytes and produced brevetoxin-like compounds, had superoxide radical production, and lipid peroxidation. With this contribution, we confirm the presence of C. subsalsa, C. marina and F. japonica in the Gulf of California. {\circledC} 2012 Springer Science+Business Media B.V.",
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Band-Schmidt, CJ, Martínez-López, A, Bustillos-Guzmán, JJ, Carreón-Palau, L, Morquecho, L, Olguín-Monroy, NO, Zenteno-Savín, T, Mendoza-Flores, A, González-Acosta, B, Hernández-Sandoval, FH & Tomas, C 2012, 'Morphology, biochemistry, and growth of raphidophyte strains from the Gulf of California', Hydrobiologia, pp. 81-97. https://doi.org/10.1007/s10750-012-1088-y

Morphology, biochemistry, and growth of raphidophyte strains from the Gulf of California. / Band-Schmidt, C. J.; Martínez-López, A.; Bustillos-Guzmán, J. J.; Carreón-Palau, L.; Morquecho, L.; Olguín-Monroy, N. O.; Zenteno-Savín, T.; Mendoza-Flores, A.; González-Acosta, B.; Hernández-Sandoval, F. H.; Tomas, C.

In: Hydrobiologia, 01.09.2012, p. 81-97.

Research output: Contribution to journalArticle

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T1 - Morphology, biochemistry, and growth of raphidophyte strains from the Gulf of California

AU - Band-Schmidt, C. J.

AU - Martínez-López, A.

AU - Bustillos-Guzmán, J. J.

AU - Carreón-Palau, L.

AU - Morquecho, L.

AU - Olguín-Monroy, N. O.

AU - Zenteno-Savín, T.

AU - Mendoza-Flores, A.

AU - González-Acosta, B.

AU - Hernández-Sandoval, F. H.

AU - Tomas, C.

PY - 2012/9/1

Y1 - 2012/9/1

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