TY - JOUR
T1 - Morphology, biochemistry, and growth of raphidophyte strains from the Gulf of California
AU - Band-Schmidt, C. J.
AU - Martínez-López, A.
AU - Bustillos-Guzmán, J. J.
AU - Carreón-Palau, L.
AU - Morquecho, L.
AU - Olguín-Monroy, N. O.
AU - Zenteno-Savín, T.
AU - Mendoza-Flores, A.
AU - González-Acosta, B.
AU - Hernández-Sandoval, F. H.
AU - Tomas, C.
N1 - Funding Information:
Acknowledgments The authors thank Ira Fogel of the Centro de Investigaciones Biológicas del Noroeste (CIBNOR) for editorial improvements. Research was supported by Consejo Nacional de Ciencia y Tecnología (CONACYT Grant 61226 and SIP Grant 20110590). C.J.B.S. and A.M.L. are fellows of the Comisión de Operación y Fomento de Actividades Académicas (COFFA) and the Programa de Estímulos al Desempeño de los Investigadores (EDI) of the Instituto Politécnico Nacional.
PY - 2012/9
Y1 - 2012/9
N2 - Strains of raphidophytes from different regions of the Gulf of California were established for identification and biochemical description. Raphidophyte in our coasts have been traditionally identified based on microscopic observations, a biochemical analyses of strains present in our coast is needed for a more detailed characterization and species confirmation. Strains were identified by morphological observations and sequencing of the 5. 8S and COI regions. Cells were cultivated in modified f/2 media at 20°C with a light-dark cycle (12 h:12 h) and 150 μmol m -2 s -1 light intensity. Pigments were identified by HPLC, brevetoxins by LC-MS/MS, fatty acids by gas chromatography, superoxide radicals by spectrophotometry, and lipid peroxidation by the determination of thiobarbituric acid reactive substances. Strains were identified as Chattonella subsalsa, C. marina, and Fibrocapsa japonica. In all strains, the main pigment was chlorophyll a, followed by fucoxanthin, chlorophyll c1 and c2, violaxanthin, β-carotene, and diadinoxanthin. Strains were tested for PbTx-1, PbTx-2, PbTx-3, PbTx-6, PbTx-9, PbTx-carboxylic acid, brevenal, and brevisin; none were detected. All strains presented superoxide radical production and lipid peroxidation. The main fatty acids were 18:4 (n-3) and 20:5 (n-3). Strains had typical fatty acid composition for raphidophytes and produced brevetoxin-like compounds, had superoxide radical production, and lipid peroxidation. With this contribution, we confirm the presence of C. subsalsa, C. marina and F. japonica in the Gulf of California.
AB - Strains of raphidophytes from different regions of the Gulf of California were established for identification and biochemical description. Raphidophyte in our coasts have been traditionally identified based on microscopic observations, a biochemical analyses of strains present in our coast is needed for a more detailed characterization and species confirmation. Strains were identified by morphological observations and sequencing of the 5. 8S and COI regions. Cells were cultivated in modified f/2 media at 20°C with a light-dark cycle (12 h:12 h) and 150 μmol m -2 s -1 light intensity. Pigments were identified by HPLC, brevetoxins by LC-MS/MS, fatty acids by gas chromatography, superoxide radicals by spectrophotometry, and lipid peroxidation by the determination of thiobarbituric acid reactive substances. Strains were identified as Chattonella subsalsa, C. marina, and Fibrocapsa japonica. In all strains, the main pigment was chlorophyll a, followed by fucoxanthin, chlorophyll c1 and c2, violaxanthin, β-carotene, and diadinoxanthin. Strains were tested for PbTx-1, PbTx-2, PbTx-3, PbTx-6, PbTx-9, PbTx-carboxylic acid, brevenal, and brevisin; none were detected. All strains presented superoxide radical production and lipid peroxidation. The main fatty acids were 18:4 (n-3) and 20:5 (n-3). Strains had typical fatty acid composition for raphidophytes and produced brevetoxin-like compounds, had superoxide radical production, and lipid peroxidation. With this contribution, we confirm the presence of C. subsalsa, C. marina and F. japonica in the Gulf of California.
KW - Chattonella
KW - Fatty acids
KW - Fibrocapsa japonica
KW - Raphidophytes
KW - Sequences
KW - Superoxide
UR - http://www.scopus.com/inward/record.url?scp=84864304034&partnerID=8YFLogxK
U2 - 10.1007/s10750-012-1088-y
DO - 10.1007/s10750-012-1088-y
M3 - Artículo
SN - 0018-8158
VL - 693
SP - 81
EP - 97
JO - Hydrobiologia
JF - Hydrobiologia
IS - 1
ER -