Monoterpenoid oxindole alkaloid production by Uncaria tomentosa (Willd) D.C. cell suspension cultures in a stirred tank bioreactor

Gabriela Trejo-Tapia, Carlos M. Cerda-García-Rojas, Mario Rodríguez-Monroy, Ana C. Ramos-Valdivia

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10 Citations (Scopus)

Abstract

Cell growth, monoterpenoid oxindole alkaloid (MOA) production, and morphological properties of Uncaria tomentosa cell suspension cultures in a 2-L stirred tank bioreactor were investigated. U. tomentosa (cell line green Uth-3) was able to grow in a stirred tank at an impeller tip speed of 95 cm/s (agitation speed of 400 rpm), showing a maximum biomass yield of 11.9 ± 0.6 g DW/L and a specific growth rate of 0.102 d-1. U. tomentosa cells growing in a stirred tank achieved maximum volumetric and specific MOA concentration (467.7 ± 40.0 μg/L, 44.6 ± 5.2 μg/g DW) at 16 days of culture. MOA chemical profile of cell suspension cultures growing in a stirred tank resembled that of the plant. Depending on culture time, from the total MOA produced, 37-100% was found in the medium in the bioreactor culture. MOA concentration achieved in a stirred tank was up to 10-fold higher than that obtained in Erlenmeyer flasks (agitated at 110 rpm). In a stirred tank, average area of the single cells of U. tomentosa increased up to 4-fold, and elliptical form factor increased from 1.40 to 2.55, indicating enlargement of U. tomentosa single cells. This work presents the first report of U. tomentosa green cell suspension cultures that grow and produce MOA in a stirred tank bioreactor. © 2005 American Chemical Society and American Institute of Chemical Engineers.
Original languageAmerican English
Pages (from-to)786-792
Number of pages7
JournalBiotechnology Progress
DOIs
StatePublished - 1 May 2005

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Cat's Claw
Monoterpenes
Bioreactors
Alkaloids
Suspensions
Cell Culture Techniques
Growth
Biomass
oxindole
Cell Line

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title = "Monoterpenoid oxindole alkaloid production by Uncaria tomentosa (Willd) D.C. cell suspension cultures in a stirred tank bioreactor",
abstract = "Cell growth, monoterpenoid oxindole alkaloid (MOA) production, and morphological properties of Uncaria tomentosa cell suspension cultures in a 2-L stirred tank bioreactor were investigated. U. tomentosa (cell line green Uth-3) was able to grow in a stirred tank at an impeller tip speed of 95 cm/s (agitation speed of 400 rpm), showing a maximum biomass yield of 11.9 ± 0.6 g DW/L and a specific growth rate of 0.102 d-1. U. tomentosa cells growing in a stirred tank achieved maximum volumetric and specific MOA concentration (467.7 ± 40.0 μg/L, 44.6 ± 5.2 μg/g DW) at 16 days of culture. MOA chemical profile of cell suspension cultures growing in a stirred tank resembled that of the plant. Depending on culture time, from the total MOA produced, 37-100{\%} was found in the medium in the bioreactor culture. MOA concentration achieved in a stirred tank was up to 10-fold higher than that obtained in Erlenmeyer flasks (agitated at 110 rpm). In a stirred tank, average area of the single cells of U. tomentosa increased up to 4-fold, and elliptical form factor increased from 1.40 to 2.55, indicating enlargement of U. tomentosa single cells. This work presents the first report of U. tomentosa green cell suspension cultures that grow and produce MOA in a stirred tank bioreactor. {\circledC} 2005 American Chemical Society and American Institute of Chemical Engineers.",
author = "Gabriela Trejo-Tapia and Cerda-Garc{\'i}a-Rojas, {Carlos M.} and Mario Rodr{\'i}guez-Monroy and Ramos-Valdivia, {Ana C.}",
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month = "5",
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doi = "10.1021/bp049608s",
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Monoterpenoid oxindole alkaloid production by Uncaria tomentosa (Willd) D.C. cell suspension cultures in a stirred tank bioreactor. / Trejo-Tapia, Gabriela; Cerda-García-Rojas, Carlos M.; Rodríguez-Monroy, Mario; Ramos-Valdivia, Ana C.

In: Biotechnology Progress, 01.05.2005, p. 786-792.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Monoterpenoid oxindole alkaloid production by Uncaria tomentosa (Willd) D.C. cell suspension cultures in a stirred tank bioreactor

AU - Trejo-Tapia, Gabriela

AU - Cerda-García-Rojas, Carlos M.

AU - Rodríguez-Monroy, Mario

AU - Ramos-Valdivia, Ana C.

PY - 2005/5/1

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N2 - Cell growth, monoterpenoid oxindole alkaloid (MOA) production, and morphological properties of Uncaria tomentosa cell suspension cultures in a 2-L stirred tank bioreactor were investigated. U. tomentosa (cell line green Uth-3) was able to grow in a stirred tank at an impeller tip speed of 95 cm/s (agitation speed of 400 rpm), showing a maximum biomass yield of 11.9 ± 0.6 g DW/L and a specific growth rate of 0.102 d-1. U. tomentosa cells growing in a stirred tank achieved maximum volumetric and specific MOA concentration (467.7 ± 40.0 μg/L, 44.6 ± 5.2 μg/g DW) at 16 days of culture. MOA chemical profile of cell suspension cultures growing in a stirred tank resembled that of the plant. Depending on culture time, from the total MOA produced, 37-100% was found in the medium in the bioreactor culture. MOA concentration achieved in a stirred tank was up to 10-fold higher than that obtained in Erlenmeyer flasks (agitated at 110 rpm). In a stirred tank, average area of the single cells of U. tomentosa increased up to 4-fold, and elliptical form factor increased from 1.40 to 2.55, indicating enlargement of U. tomentosa single cells. This work presents the first report of U. tomentosa green cell suspension cultures that grow and produce MOA in a stirred tank bioreactor. © 2005 American Chemical Society and American Institute of Chemical Engineers.

AB - Cell growth, monoterpenoid oxindole alkaloid (MOA) production, and morphological properties of Uncaria tomentosa cell suspension cultures in a 2-L stirred tank bioreactor were investigated. U. tomentosa (cell line green Uth-3) was able to grow in a stirred tank at an impeller tip speed of 95 cm/s (agitation speed of 400 rpm), showing a maximum biomass yield of 11.9 ± 0.6 g DW/L and a specific growth rate of 0.102 d-1. U. tomentosa cells growing in a stirred tank achieved maximum volumetric and specific MOA concentration (467.7 ± 40.0 μg/L, 44.6 ± 5.2 μg/g DW) at 16 days of culture. MOA chemical profile of cell suspension cultures growing in a stirred tank resembled that of the plant. Depending on culture time, from the total MOA produced, 37-100% was found in the medium in the bioreactor culture. MOA concentration achieved in a stirred tank was up to 10-fold higher than that obtained in Erlenmeyer flasks (agitated at 110 rpm). In a stirred tank, average area of the single cells of U. tomentosa increased up to 4-fold, and elliptical form factor increased from 1.40 to 2.55, indicating enlargement of U. tomentosa single cells. This work presents the first report of U. tomentosa green cell suspension cultures that grow and produce MOA in a stirred tank bioreactor. © 2005 American Chemical Society and American Institute of Chemical Engineers.

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