Monoterpenoid indole alkaloids and phenols are required antioxidants in glutathione depleted Uncaria tomentosa root cultures

Ileana Vera-Reyes, Ariana A. Huerta-Heredia, Teresa Ponce-Noyola, Carlos M. Cerda-García-Rojas, Gabriela Trejo-Tapia, Ana C. Ramos-Valdivia

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Plants cells sense their environment through oxidative signaling responses and make appropriate adjustments to gene expression, physiology and metabolic defense. Root cultures of Uncaria tomentosa, a native plant of the Amazon rainforest, were exposed to stressful conditions by combined addition of the glutathione inhibitor, buthionine sulfoximine (0.8 mM) and 0.2 mM jasmonic acid. This procedure induced a synchronized two-fold increase of hydrogen peroxide and guaiacol peroxidases, while the glutathione content and glutathione reductase activity were reduced. Likewise, in elicited cultures, production of the antioxidant secondary metabolites, monoterpenoid oxindole, and glucoindole alkaloids, were 2.1 and 5.5-fold stimulated (704.0 ± 14.9 and 845.5 ± 13.0 μg/g DW, respectively) after 12 h, while phenols were three times increased. Upon elicitation, the activities and mRNA transcript levels of two enzymes involved in the alkaloid biosynthesis, strictosidine synthase and strictosidine β-glucosidase, were also enhanced. Differential proteome analysis performed by two-dimensional polyacrylamide gel electrophoresis of elicited and control root cultures showed that after elicitation several new protein spots appeared. Two of them were identified as thiol-related enzymes, namely cysteine synthase and methionine synthase. Proteins associated with antioxidant and stress responses, including two strictosidine synthase isoforms, were identified as well, together with others as caffeic acid O-methyltransferase. Our results propose that in U. tomentosa roots a signaling network involving hydrogen peroxide and jasmonate derivatives coordinately regulates the antioxidant response and secondary metabolic defense via transcriptional and protein activation.

Original languageEnglish
Article number27
JournalFrontiers in Environmental Science
Volume3
Issue numberAPR
DOIs
StatePublished - 30 Apr 2015

Fingerprint

alkaloid
antioxidant
phenol
hydrogen peroxide
protein
enzyme
fold
thiol
acid
secondary metabolite
rainforest
gene expression
physiology
inhibitor
electrokinesis
gel
defence

Keywords

  • Antioxidant responses
  • Glutathione
  • Oxidative stress
  • Proteome
  • Uncaria tomentosa

Cite this

Vera-Reyes, Ileana ; Huerta-Heredia, Ariana A. ; Ponce-Noyola, Teresa ; Cerda-García-Rojas, Carlos M. ; Trejo-Tapia, Gabriela ; Ramos-Valdivia, Ana C. / Monoterpenoid indole alkaloids and phenols are required antioxidants in glutathione depleted Uncaria tomentosa root cultures. In: Frontiers in Environmental Science. 2015 ; Vol. 3, No. APR.
@article{f138bc4d52c94b22aca68efed4e65b53,
title = "Monoterpenoid indole alkaloids and phenols are required antioxidants in glutathione depleted Uncaria tomentosa root cultures",
abstract = "Plants cells sense their environment through oxidative signaling responses and make appropriate adjustments to gene expression, physiology and metabolic defense. Root cultures of Uncaria tomentosa, a native plant of the Amazon rainforest, were exposed to stressful conditions by combined addition of the glutathione inhibitor, buthionine sulfoximine (0.8 mM) and 0.2 mM jasmonic acid. This procedure induced a synchronized two-fold increase of hydrogen peroxide and guaiacol peroxidases, while the glutathione content and glutathione reductase activity were reduced. Likewise, in elicited cultures, production of the antioxidant secondary metabolites, monoterpenoid oxindole, and glucoindole alkaloids, were 2.1 and 5.5-fold stimulated (704.0 ± 14.9 and 845.5 ± 13.0 μg/g DW, respectively) after 12 h, while phenols were three times increased. Upon elicitation, the activities and mRNA transcript levels of two enzymes involved in the alkaloid biosynthesis, strictosidine synthase and strictosidine β-glucosidase, were also enhanced. Differential proteome analysis performed by two-dimensional polyacrylamide gel electrophoresis of elicited and control root cultures showed that after elicitation several new protein spots appeared. Two of them were identified as thiol-related enzymes, namely cysteine synthase and methionine synthase. Proteins associated with antioxidant and stress responses, including two strictosidine synthase isoforms, were identified as well, together with others as caffeic acid O-methyltransferase. Our results propose that in U. tomentosa roots a signaling network involving hydrogen peroxide and jasmonate derivatives coordinately regulates the antioxidant response and secondary metabolic defense via transcriptional and protein activation.",
keywords = "Antioxidant responses, Glutathione, Oxidative stress, Proteome, Uncaria tomentosa",
author = "Ileana Vera-Reyes and Huerta-Heredia, {Ariana A.} and Teresa Ponce-Noyola and Cerda-Garc{\'i}a-Rojas, {Carlos M.} and Gabriela Trejo-Tapia and Ramos-Valdivia, {Ana C.}",
year = "2015",
month = "4",
day = "30",
doi = "10.3389/fenvs.2015.00027",
language = "Ingl{\'e}s",
volume = "3",
journal = "Frontiers in Environmental Science",
issn = "2296-665X",
number = "APR",

}

Monoterpenoid indole alkaloids and phenols are required antioxidants in glutathione depleted Uncaria tomentosa root cultures. / Vera-Reyes, Ileana; Huerta-Heredia, Ariana A.; Ponce-Noyola, Teresa; Cerda-García-Rojas, Carlos M.; Trejo-Tapia, Gabriela; Ramos-Valdivia, Ana C.

In: Frontiers in Environmental Science, Vol. 3, No. APR, 27, 30.04.2015.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Monoterpenoid indole alkaloids and phenols are required antioxidants in glutathione depleted Uncaria tomentosa root cultures

AU - Vera-Reyes, Ileana

AU - Huerta-Heredia, Ariana A.

AU - Ponce-Noyola, Teresa

AU - Cerda-García-Rojas, Carlos M.

AU - Trejo-Tapia, Gabriela

AU - Ramos-Valdivia, Ana C.

PY - 2015/4/30

Y1 - 2015/4/30

N2 - Plants cells sense their environment through oxidative signaling responses and make appropriate adjustments to gene expression, physiology and metabolic defense. Root cultures of Uncaria tomentosa, a native plant of the Amazon rainforest, were exposed to stressful conditions by combined addition of the glutathione inhibitor, buthionine sulfoximine (0.8 mM) and 0.2 mM jasmonic acid. This procedure induced a synchronized two-fold increase of hydrogen peroxide and guaiacol peroxidases, while the glutathione content and glutathione reductase activity were reduced. Likewise, in elicited cultures, production of the antioxidant secondary metabolites, monoterpenoid oxindole, and glucoindole alkaloids, were 2.1 and 5.5-fold stimulated (704.0 ± 14.9 and 845.5 ± 13.0 μg/g DW, respectively) after 12 h, while phenols were three times increased. Upon elicitation, the activities and mRNA transcript levels of two enzymes involved in the alkaloid biosynthesis, strictosidine synthase and strictosidine β-glucosidase, were also enhanced. Differential proteome analysis performed by two-dimensional polyacrylamide gel electrophoresis of elicited and control root cultures showed that after elicitation several new protein spots appeared. Two of them were identified as thiol-related enzymes, namely cysteine synthase and methionine synthase. Proteins associated with antioxidant and stress responses, including two strictosidine synthase isoforms, were identified as well, together with others as caffeic acid O-methyltransferase. Our results propose that in U. tomentosa roots a signaling network involving hydrogen peroxide and jasmonate derivatives coordinately regulates the antioxidant response and secondary metabolic defense via transcriptional and protein activation.

AB - Plants cells sense their environment through oxidative signaling responses and make appropriate adjustments to gene expression, physiology and metabolic defense. Root cultures of Uncaria tomentosa, a native plant of the Amazon rainforest, were exposed to stressful conditions by combined addition of the glutathione inhibitor, buthionine sulfoximine (0.8 mM) and 0.2 mM jasmonic acid. This procedure induced a synchronized two-fold increase of hydrogen peroxide and guaiacol peroxidases, while the glutathione content and glutathione reductase activity were reduced. Likewise, in elicited cultures, production of the antioxidant secondary metabolites, monoterpenoid oxindole, and glucoindole alkaloids, were 2.1 and 5.5-fold stimulated (704.0 ± 14.9 and 845.5 ± 13.0 μg/g DW, respectively) after 12 h, while phenols were three times increased. Upon elicitation, the activities and mRNA transcript levels of two enzymes involved in the alkaloid biosynthesis, strictosidine synthase and strictosidine β-glucosidase, were also enhanced. Differential proteome analysis performed by two-dimensional polyacrylamide gel electrophoresis of elicited and control root cultures showed that after elicitation several new protein spots appeared. Two of them were identified as thiol-related enzymes, namely cysteine synthase and methionine synthase. Proteins associated with antioxidant and stress responses, including two strictosidine synthase isoforms, were identified as well, together with others as caffeic acid O-methyltransferase. Our results propose that in U. tomentosa roots a signaling network involving hydrogen peroxide and jasmonate derivatives coordinately regulates the antioxidant response and secondary metabolic defense via transcriptional and protein activation.

KW - Antioxidant responses

KW - Glutathione

KW - Oxidative stress

KW - Proteome

KW - Uncaria tomentosa

UR - http://www.scopus.com/inward/record.url?scp=84975917536&partnerID=8YFLogxK

U2 - 10.3389/fenvs.2015.00027

DO - 10.3389/fenvs.2015.00027

M3 - Artículo

AN - SCOPUS:84975917536

VL - 3

JO - Frontiers in Environmental Science

JF - Frontiers in Environmental Science

SN - 2296-665X

IS - APR

M1 - 27

ER -