Molecular diversity and phylogeny of rhizobia associated with Lablab purpureus (Linn.) grown in Southern China

Yue Li Chang, En Tao Wang, Xin Hua Sui, Xiao Xia Zhang, Wen Xin Chen

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8 Citations (Scopus)

Abstract

As an introduced plant, Lablab purpureus serves as a vegetable, herbal medicine, forage and green manure in China. In order to investigate the diversity of rhizobia associated with this plant, a total of 49 rhizobial strains isolated from ten provinces of Southern China were analyzed in the present study with restriction fragment length polymorphism and/or sequence analyses of housekeeping genes (16S rRNA, IGS, atpD, glnII and recA) and symbiotic genes (nifH and nodC). The results defined the L. purpureus rhizobia as 24 IGS-types within 15 rrs-IGS clusters or genomic species belonging to Bradyrhizobium, Rhizobium, Ensifer (synonym of Sinorhizobium) and Mesorhizobium. Bradyrhizobium spp. (81.6%) were the most abundant isolates, half of which were B. elkanii. Most of these rhizobia induced nodules on L. purpureus, but symbiotic genes were only amplified from the Bradyrhizobium and Rhizobium leguminosarum strains. The nodC and nifH phylogenetic trees defined five lineages corresponding to B. yuanmingense, B. japonicum, B. elkanii, B. jicamae and R. leguminosarum. The coherence of housekeeping and symbiotic gene phylogenies demonstrated that the symbiotic genes of the Lablab rhizobia were maintained mainly through vertical transfer. However, a putative lateral transfer of symbiotic genes was found in the B. liaoningense strain. The results in the present study clearly revealed that L. purpureus was a promiscuous host that formed nodules with diverse rhizobia, mainly Bradyrhizobium species, harboring different symbiotic genes. © 2011 Elsevier GmbH.
Original languageAmerican English
Pages (from-to)276-284
Number of pages247
JournalSystematic and Applied Microbiology
DOIs
StatePublished - 1 Jun 2011

Fingerprint

Lablab purpureus
Rhizobium
Phylogeny
rhizobacterium
Bradyrhizobium
China
phylogeny
gene
Bradyrhizobium elkanii
Rhizobium leguminosarum
genes
Essential Genes
Genes
Sinorhizobium
Mesorhizobium
Ensifer
Lablab
Horizontal Gene Transfer
Bradyrhizobium japonicum
Manure

Cite this

Chang, Yue Li ; Wang, En Tao ; Sui, Xin Hua ; Zhang, Xiao Xia ; Chen, Wen Xin. / Molecular diversity and phylogeny of rhizobia associated with Lablab purpureus (Linn.) grown in Southern China. In: Systematic and Applied Microbiology. 2011 ; pp. 276-284.
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abstract = "As an introduced plant, Lablab purpureus serves as a vegetable, herbal medicine, forage and green manure in China. In order to investigate the diversity of rhizobia associated with this plant, a total of 49 rhizobial strains isolated from ten provinces of Southern China were analyzed in the present study with restriction fragment length polymorphism and/or sequence analyses of housekeeping genes (16S rRNA, IGS, atpD, glnII and recA) and symbiotic genes (nifH and nodC). The results defined the L. purpureus rhizobia as 24 IGS-types within 15 rrs-IGS clusters or genomic species belonging to Bradyrhizobium, Rhizobium, Ensifer (synonym of Sinorhizobium) and Mesorhizobium. Bradyrhizobium spp. (81.6{\%}) were the most abundant isolates, half of which were B. elkanii. Most of these rhizobia induced nodules on L. purpureus, but symbiotic genes were only amplified from the Bradyrhizobium and Rhizobium leguminosarum strains. The nodC and nifH phylogenetic trees defined five lineages corresponding to B. yuanmingense, B. japonicum, B. elkanii, B. jicamae and R. leguminosarum. The coherence of housekeeping and symbiotic gene phylogenies demonstrated that the symbiotic genes of the Lablab rhizobia were maintained mainly through vertical transfer. However, a putative lateral transfer of symbiotic genes was found in the B. liaoningense strain. The results in the present study clearly revealed that L. purpureus was a promiscuous host that formed nodules with diverse rhizobia, mainly Bradyrhizobium species, harboring different symbiotic genes. {\circledC} 2011 Elsevier GmbH.",
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Molecular diversity and phylogeny of rhizobia associated with Lablab purpureus (Linn.) grown in Southern China. / Chang, Yue Li; Wang, En Tao; Sui, Xin Hua; Zhang, Xiao Xia; Chen, Wen Xin.

In: Systematic and Applied Microbiology, 01.06.2011, p. 276-284.

Research output: Contribution to journalArticleResearchpeer-review

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T1 - Molecular diversity and phylogeny of rhizobia associated with Lablab purpureus (Linn.) grown in Southern China

AU - Chang, Yue Li

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AU - Chen, Wen Xin

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