TY - JOUR
T1 - Molecular diversity and phylogeny of rhizobia associated with Lablab purpureus (Linn.) grown in Southern China
AU - Chang, Yue Li
AU - Wang, En Tao
AU - Sui, Xin Hua
AU - Zhang, Xiao Xia
AU - Chen, Wen Xin
N1 - Funding Information:
The authors would like to thank the anonymous reviewers for their valuable comments. This work was supported by the National Project for Basic S&T Platform Construction (grant 2005DKA21201-10 ), the Foundation of the State Key Basic Research and Development Plan of China (grant 2010CB126500 ), and the National Natural Science Foundation of China (project no. 30970004 ). ETW was partially supported by grants SIP20100067 , awarded by IPN , and PICS08-3 , awarded by ICyT DF of Mexico.
PY - 2011/6
Y1 - 2011/6
N2 - As an introduced plant, Lablab purpureus serves as a vegetable, herbal medicine, forage and green manure in China. In order to investigate the diversity of rhizobia associated with this plant, a total of 49 rhizobial strains isolated from ten provinces of Southern China were analyzed in the present study with restriction fragment length polymorphism and/or sequence analyses of housekeeping genes (16S rRNA, IGS, atpD, glnII and recA) and symbiotic genes (nifH and nodC). The results defined the L. purpureus rhizobia as 24 IGS-types within 15 rrs-IGS clusters or genomic species belonging to Bradyrhizobium, Rhizobium, Ensifer (synonym of Sinorhizobium) and Mesorhizobium. Bradyrhizobium spp. (81.6%) were the most abundant isolates, half of which were B. elkanii. Most of these rhizobia induced nodules on L. purpureus, but symbiotic genes were only amplified from the Bradyrhizobium and Rhizobium leguminosarum strains. The nodC and nifH phylogenetic trees defined five lineages corresponding to B. yuanmingense, B. japonicum, B. elkanii, B. jicamae and R. leguminosarum. The coherence of housekeeping and symbiotic gene phylogenies demonstrated that the symbiotic genes of the Lablab rhizobia were maintained mainly through vertical transfer. However, a putative lateral transfer of symbiotic genes was found in the B. liaoningense strain. The results in the present study clearly revealed that L. purpureus was a promiscuous host that formed nodules with diverse rhizobia, mainly Bradyrhizobium species, harboring different symbiotic genes.
AB - As an introduced plant, Lablab purpureus serves as a vegetable, herbal medicine, forage and green manure in China. In order to investigate the diversity of rhizobia associated with this plant, a total of 49 rhizobial strains isolated from ten provinces of Southern China were analyzed in the present study with restriction fragment length polymorphism and/or sequence analyses of housekeeping genes (16S rRNA, IGS, atpD, glnII and recA) and symbiotic genes (nifH and nodC). The results defined the L. purpureus rhizobia as 24 IGS-types within 15 rrs-IGS clusters or genomic species belonging to Bradyrhizobium, Rhizobium, Ensifer (synonym of Sinorhizobium) and Mesorhizobium. Bradyrhizobium spp. (81.6%) were the most abundant isolates, half of which were B. elkanii. Most of these rhizobia induced nodules on L. purpureus, but symbiotic genes were only amplified from the Bradyrhizobium and Rhizobium leguminosarum strains. The nodC and nifH phylogenetic trees defined five lineages corresponding to B. yuanmingense, B. japonicum, B. elkanii, B. jicamae and R. leguminosarum. The coherence of housekeeping and symbiotic gene phylogenies demonstrated that the symbiotic genes of the Lablab rhizobia were maintained mainly through vertical transfer. However, a putative lateral transfer of symbiotic genes was found in the B. liaoningense strain. The results in the present study clearly revealed that L. purpureus was a promiscuous host that formed nodules with diverse rhizobia, mainly Bradyrhizobium species, harboring different symbiotic genes.
KW - Bradyrhizobium
KW - Diversity
KW - Lablab purpureus
KW - Phylogeny
KW - Rhizobia
UR - http://www.scopus.com/inward/record.url?scp=79955468498&partnerID=8YFLogxK
U2 - 10.1016/j.syapm.2010.12.004
DO - 10.1016/j.syapm.2010.12.004
M3 - Artículo
SN - 0723-2020
VL - 34
SP - 276
EP - 284
JO - Systematic and Applied Microbiology
JF - Systematic and Applied Microbiology
IS - 4
ER -