TY - JOUR
T1 - Detección molecular de especies de Candida en especímenes de pacientes hospitalizados
AU - Camacho-Cardoso, José Luis
AU - Martínez-Rivera, María Ángeles
AU - Manzano-Gayosso, Patricia
AU - Méndez-Tovar, Luis Javier
AU - López-Martínez, Rubén
AU - Hernández-Hernández, Francisca
N1 - Publisher Copyright:
© 2017, Academia Nacional de Medicina. All rights reserved.
PY - 2017
Y1 - 2017
N2 - Objective: To identify the most frequent Candida species in specimens from patients hospitalized in different medical centers of Mexico City, with suspected fungal infection. Methods: Specimens were grown on Sabouraud dextrose agar at 28°C for 72 h. In addition, DNA was extracted. Isolates were grown on CHROMagar Candida™, at 37°C for 48 h. The molecular identification was performed by polymerase chain reaction (PCR) using primers specific for four species. Results: Eighty one specimens were processed and included: bronchial lavage, pleural, cerebrospinal, peritoneal, ascites and bile fluids; blood, sputum, bone marrow, oro-tracheal cannula and ganglion. By culture, 30 samples (37%) were positive, and by PCR, 41 (50.6%). By PCR, the frequency of species was: Candida albicans 82.9%, Candida tropicalis 31.7%, Candida glabrata 24.4%, and Candida parapsilosis 4.9%. In 34.1% of specimens a species mixture was detected suggesting a co-infection: Two species in five specimens (C. albicans-C tropicalis and C. albicans-C glabrata), and three species in three specimens (C. albicans-C. glabrata-C. tropicalis). Conclusions: The PCR is an useful tool for detection the most common Candida species causing in- fection in hospitalized patients, it avoids the requirement of culture weather we start from clinical specimen and it favors the early diagnosis of invasive candidiasis.
AB - Objective: To identify the most frequent Candida species in specimens from patients hospitalized in different medical centers of Mexico City, with suspected fungal infection. Methods: Specimens were grown on Sabouraud dextrose agar at 28°C for 72 h. In addition, DNA was extracted. Isolates were grown on CHROMagar Candida™, at 37°C for 48 h. The molecular identification was performed by polymerase chain reaction (PCR) using primers specific for four species. Results: Eighty one specimens were processed and included: bronchial lavage, pleural, cerebrospinal, peritoneal, ascites and bile fluids; blood, sputum, bone marrow, oro-tracheal cannula and ganglion. By culture, 30 samples (37%) were positive, and by PCR, 41 (50.6%). By PCR, the frequency of species was: Candida albicans 82.9%, Candida tropicalis 31.7%, Candida glabrata 24.4%, and Candida parapsilosis 4.9%. In 34.1% of specimens a species mixture was detected suggesting a co-infection: Two species in five specimens (C. albicans-C tropicalis and C. albicans-C glabrata), and three species in three specimens (C. albicans-C. glabrata-C. tropicalis). Conclusions: The PCR is an useful tool for detection the most common Candida species causing in- fection in hospitalized patients, it avoids the requirement of culture weather we start from clinical specimen and it favors the early diagnosis of invasive candidiasis.
KW - CHROMagar Candida™
KW - Candida albicans
KW - Candida glabrata
KW - Candida spp
KW - Candida tropicalis
KW - Invasive candidiasis
KW - Polimerase chain reaction
UR - http://www.scopus.com/inward/record.url?scp=85039744767&partnerID=8YFLogxK
U2 - 10.24875/GMM.17002535
DO - 10.24875/GMM.17002535
M3 - Artículo
C2 - 29099103
SN - 0016-3813
VL - 153
SP - 581
EP - 589
JO - Gaceta Medica de Mexico
JF - Gaceta Medica de Mexico
IS - 5
ER -