Molecular and biological characterization of Watermelon chlorotic stunt virus (WmCSV): An Eastern Hemisphere begomovirus introduced in the Western Hemisphere

Gustavo Domínguez-Durán; Edgar A. Rodríguez-Negrete; Juan José Morales-Aguilar; Erika Camacho-Beltrán; Jesús L. Romero-Romero; Maria A. Rivera-Acosta; Norma E. Leyva-López; Analilia Arroyo-Becerra; Jesús Méndez-Lozano

doi:10.1016/j.cropro.2017.09.006

Molecular and biological characterization of Watermelon chlorotic stunt virus (WmCSV): An Eastern Hemisphere begomovirus introduced in the Western Hemisphere

Gustavo Domínguez-Durán, Edgar A. Rodríguez-Negrete, Juan José Morales-Aguilar, Erika Camacho-Beltrán, Jesús L. Romero-Romero, Maria A. Rivera-Acosta, Norma E. Leyva-López, Analilia Arroyo-Becerra, Jesús Méndez-Lozano

Centro Interdisciplinario de Investigación para el Desarrollo Integral Regional (CIIDIR), Unidad Sinaloa
Centro de Investigación en Biotecnología Aplicada (CIBA), Unidad Tlaxcala

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18 Scopus citations

Abstract

Watermelon chlorotic stunt virus (WmCSV) is a bipartite begomovirus that causes severe diseases to cucurbits, particularly watermelon, across Eastern Mediterranean countries. Watermelon plants showing begomovirus-associated symptoms and virus vector whitefly infestation were collected from a cultivated area in Sonora, Mexico. Using rolling circle amplification (RCA) and sequencing, the full-length genome of an isolate of WmCSV was obtained. Agrobacterium-mediated virus inoculation to Solanaceae (Nicotiana benthamiana and tomato) and Cucurbitaceae (Squash, melon and watermelon) plants, fulfilled Koch's postulates confirming the disease causal agent. To the best of our knowledge, this is the first report of WmCSV associated to cucurbit diseases in the Western Hemisphere.

Original language	English
Pages (from-to)	51-55
Number of pages	5
Journal	Crop Protection
Volume	103
DOIs	https://doi.org/10.1016/j.cropro.2017.09.006
State	Published - Jan 2018

Keywords

Begomovirus
Cucurbits
Old world (OW) virus
WmCSV

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10.1016/j.cropro.2017.09.006

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Domínguez-Durán, G., Rodríguez-Negrete, E. A., Morales-Aguilar, J. J., Camacho-Beltrán, E., Romero-Romero, J. L., Rivera-Acosta, M. A., Leyva-López, N. E., Arroyo-Becerra, A., & Méndez-Lozano, J. (2018). Molecular and biological characterization of Watermelon chlorotic stunt virus (WmCSV): An Eastern Hemisphere begomovirus introduced in the Western Hemisphere. Crop Protection, 103, 51-55. https://doi.org/10.1016/j.cropro.2017.09.006

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title = "Molecular and biological characterization of Watermelon chlorotic stunt virus (WmCSV): An Eastern Hemisphere begomovirus introduced in the Western Hemisphere",

abstract = "Watermelon chlorotic stunt virus (WmCSV) is a bipartite begomovirus that causes severe diseases to cucurbits, particularly watermelon, across Eastern Mediterranean countries. Watermelon plants showing begomovirus-associated symptoms and virus vector whitefly infestation were collected from a cultivated area in Sonora, Mexico. Using rolling circle amplification (RCA) and sequencing, the full-length genome of an isolate of WmCSV was obtained. Agrobacterium-mediated virus inoculation to Solanaceae (Nicotiana benthamiana and tomato) and Cucurbitaceae (Squash, melon and watermelon) plants, fulfilled Koch's postulates confirming the disease causal agent. To the best of our knowledge, this is the first report of WmCSV associated to cucurbit diseases in the Western Hemisphere.",

keywords = "Begomovirus, Cucurbits, Old world (OW) virus, WmCSV",

author = "Gustavo Dom{\'i}nguez-Dur{\'a}n and Rodr{\'i}guez-Negrete, {Edgar A.} and Morales-Aguilar, {Juan Jos{\'e}} and Erika Camacho-Beltr{\'a}n and Romero-Romero, {Jes{\'u}s L.} and Rivera-Acosta, {Maria A.} and Leyva-L{\'o}pez, {Norma E.} and Analilia Arroyo-Becerra and Jes{\'u}s M{\'e}ndez-Lozano",

note = "Publisher Copyright: {\textcopyright} 2017 Elsevier Ltd",

year = "2018",

month = jan,

doi = "10.1016/j.cropro.2017.09.006",

language = "Ingl{\'e}s",

volume = "103",

pages = "51--55",

journal = "Crop Protection",

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Domínguez-Durán, G, Rodríguez-Negrete, EA, Morales-Aguilar, JJ, Camacho-Beltrán, E , Romero-Romero, JL, Rivera-Acosta, MA, Leyva-López, NE , Arroyo-Becerra, A & Méndez-Lozano, J 2018, 'Molecular and biological characterization of Watermelon chlorotic stunt virus (WmCSV): An Eastern Hemisphere begomovirus introduced in the Western Hemisphere', Crop Protection, vol. 103, pp. 51-55. https://doi.org/10.1016/j.cropro.2017.09.006

Molecular and biological characterization of Watermelon chlorotic stunt virus (WmCSV): An Eastern Hemisphere begomovirus introduced in the Western Hemisphere. / Domínguez-Durán, Gustavo; Rodríguez-Negrete, Edgar A.; Morales-Aguilar, Juan José et al.
In: Crop Protection, Vol. 103, 01.2018, p. 51-55.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Molecular and biological characterization of Watermelon chlorotic stunt virus (WmCSV)

T2 - An Eastern Hemisphere begomovirus introduced in the Western Hemisphere

AU - Domínguez-Durán, Gustavo

AU - Rodríguez-Negrete, Edgar A.

AU - Morales-Aguilar, Juan José

AU - Camacho-Beltrán, Erika

AU - Romero-Romero, Jesús L.

AU - Rivera-Acosta, Maria A.

AU - Leyva-López, Norma E.

AU - Arroyo-Becerra, Analilia

AU - Méndez-Lozano, Jesús

PY - 2018/1

Y1 - 2018/1

N2 - Watermelon chlorotic stunt virus (WmCSV) is a bipartite begomovirus that causes severe diseases to cucurbits, particularly watermelon, across Eastern Mediterranean countries. Watermelon plants showing begomovirus-associated symptoms and virus vector whitefly infestation were collected from a cultivated area in Sonora, Mexico. Using rolling circle amplification (RCA) and sequencing, the full-length genome of an isolate of WmCSV was obtained. Agrobacterium-mediated virus inoculation to Solanaceae (Nicotiana benthamiana and tomato) and Cucurbitaceae (Squash, melon and watermelon) plants, fulfilled Koch's postulates confirming the disease causal agent. To the best of our knowledge, this is the first report of WmCSV associated to cucurbit diseases in the Western Hemisphere.

AB - Watermelon chlorotic stunt virus (WmCSV) is a bipartite begomovirus that causes severe diseases to cucurbits, particularly watermelon, across Eastern Mediterranean countries. Watermelon plants showing begomovirus-associated symptoms and virus vector whitefly infestation were collected from a cultivated area in Sonora, Mexico. Using rolling circle amplification (RCA) and sequencing, the full-length genome of an isolate of WmCSV was obtained. Agrobacterium-mediated virus inoculation to Solanaceae (Nicotiana benthamiana and tomato) and Cucurbitaceae (Squash, melon and watermelon) plants, fulfilled Koch's postulates confirming the disease causal agent. To the best of our knowledge, this is the first report of WmCSV associated to cucurbit diseases in the Western Hemisphere.

KW - Begomovirus

KW - Cucurbits

KW - Old world (OW) virus

KW - WmCSV

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DO - 10.1016/j.cropro.2017.09.006

M3 - Artículo

SN - 0261-2194

VL - 103

SP - 51

EP - 55

JO - Crop Protection

JF - Crop Protection

ER -

Molecular and biological characterization of Watermelon chlorotic stunt virus (WmCSV): An Eastern Hemisphere begomovirus introduced in the Western Hemisphere

Abstract

Keywords

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