TY - JOUR
T1 - Molecular and biochemical characterisation of antagonistic mechanisms of the biocontrol agent Bacillus cereus B25 inhibiting the growth of the phytopathogen Fusarium verticillioides P03 during their direct interaction in vitro
AU - Báez-Astorga, Paúl Alán
AU - Cázares-Álvarez, Jesús Eduardo
AU - Cruz-Mendívil, Abraham
AU - Quiroz-Figueroa, Francisco Roberto
AU - Sánchez-Valle, Valeria Isabel
AU - Maldonado-Mendoza, Ignacio Eduardo
N1 - Publisher Copyright:
© 2022 Informa UK Limited, trading as Taylor & Francis Group.
PY - 2022
Y1 - 2022
N2 - Fusarium verticillioides (Fv) is a mycotoxin-producing phytopathogen causing ear and root rot in maize. Bacillus cereus strain B25 is a maize endophyte and an effective biological control agent against Fv. The B25 genome contains several genes associated with fungal antagonism, including lytic enzymes (chitinases A and B, chitosanase and glycoside hydrolase), siderophores (petrobactin and bacillibactin), antibiotics (surfactin), and biofilm. This study aimed to elucidate which B25 antagonistic mechanisms are implicated in Fv biocontrol. The expression of twelve B25 genes was evaluated by qRT-PCR after 3–30 h of confrontation between B25 and Fv. All B25 genes tested were induced, mainly between 6 and 24 h, while none was induced at 3 h. Lytic enzyme genes were induced at different times, with three of them occurring at 12 h. Chitinase and chitosanase activities were induced after 6 and 12 h, respectively, and gradually increased until 30 h. Biofilm formation was detected only when B25 interacted with Fv. Images of the B25-Fv interaction and measurements at 24 and 30 h showed little branching or abnormal mycelial growth, suggesting cell wall damage. In conclusion, when B25 is confronted against Fv, all B25 antagonistic mechanisms tested appear to be involved in Fv growth control.
AB - Fusarium verticillioides (Fv) is a mycotoxin-producing phytopathogen causing ear and root rot in maize. Bacillus cereus strain B25 is a maize endophyte and an effective biological control agent against Fv. The B25 genome contains several genes associated with fungal antagonism, including lytic enzymes (chitinases A and B, chitosanase and glycoside hydrolase), siderophores (petrobactin and bacillibactin), antibiotics (surfactin), and biofilm. This study aimed to elucidate which B25 antagonistic mechanisms are implicated in Fv biocontrol. The expression of twelve B25 genes was evaluated by qRT-PCR after 3–30 h of confrontation between B25 and Fv. All B25 genes tested were induced, mainly between 6 and 24 h, while none was induced at 3 h. Lytic enzyme genes were induced at different times, with three of them occurring at 12 h. Chitinase and chitosanase activities were induced after 6 and 12 h, respectively, and gradually increased until 30 h. Biofilm formation was detected only when B25 interacted with Fv. Images of the B25-Fv interaction and measurements at 24 and 30 h showed little branching or abnormal mycelial growth, suggesting cell wall damage. In conclusion, when B25 is confronted against Fv, all B25 antagonistic mechanisms tested appear to be involved in Fv growth control.
KW - Bacillus cereus
KW - Biocontrol
KW - Fusarium verticillioides
KW - bacterium-fungus interaction
KW - cell wall-degrading enzymes
KW - qRT-PCR
UR - http://www.scopus.com/inward/record.url?scp=85131692088&partnerID=8YFLogxK
U2 - 10.1080/09583157.2022.2085662
DO - 10.1080/09583157.2022.2085662
M3 - Artículo
AN - SCOPUS:85131692088
SN - 0958-3157
VL - 32
SP - 1074
EP - 1094
JO - Biocontrol Science and Technology
JF - Biocontrol Science and Technology
IS - 9
ER -