TY - JOUR
T1 - Modulation of tolerance to Cr(VI) and Cr(VI) reduction by sulfate ion in a Candida yeast strain isolated from tannery wastewater
AU - Guillén-Jiménez, Flor De María
AU - Morales-Barrera, Liliana
AU - Morales-Jiménez, Jesús
AU - Hernández-Rodríguez, César Hugo
AU - Cristiani-Urbina, Eliseo
N1 - Funding Information:
C. H. H.-R. and E. C.-U. are fellow holders of a grant from the Comisión de Operación y Fomento de Actividades Académicas, Instituto Politécnico Nacional, Mexico City, Mexico. The authors gratefully acknowledge the financial support provided by the Secretaría de Investigación y Posgrado, IPN. The CONACyT awarded a graduate scholarship to one of the co-authors (F. M. G.-J.).
PY - 2008/11
Y1 - 2008/11
N2 - The main aim of this study was to investigate the influence of the sulfate ion on the tolerance to Cr(VI) and the Cr(VI) reduction in a yeast strain isolated from tannery wastewater and identified as Candida sp. FGSFEP by the D1/D2 domain sequence of the 26S rRNA gene. The Candida sp. FGSFEP strain was grown in culture media with sulfate concentrations ranging from 0 to 23.92 mM, in absence and presence of Cr(VI) [1.7 and 3.3 mM]. In absence of Cr(VI), the yeast specific growth rate was practically the same in every sulfate concentration tested, which suggests that sulfate had no stimulating or inhibiting effect on the yeast cell growth. In contrast, at the two initial Cr(VI) concentrations assayed, the specific growth rate of Candida sp. FGSFEP rose when sulfate concentration increased. Likewise, the greater efficiencies and volumetric rates of Cr(VI) reduction exhibited by Candida sp. FGSFEP were obtained at high sulfate concentrations. Yeast was capable of reducing 100% of 1.7 mM Cr(VI) and 84% of 3.3 mM Cr(VI), with rates of 0.98 and 0.44 mg Cr(VI)/L h, with 10 and 23.92 mM sulfate concentrations, respectively. These results indicate that sulfate plays an important role in the tolerance to Cr(VI) and Cr(VI) reduction in Candida sp. FGSFEP. These findings may have significant implications in the biological treatment of Cr(VI)-laden wastewaters.
AB - The main aim of this study was to investigate the influence of the sulfate ion on the tolerance to Cr(VI) and the Cr(VI) reduction in a yeast strain isolated from tannery wastewater and identified as Candida sp. FGSFEP by the D1/D2 domain sequence of the 26S rRNA gene. The Candida sp. FGSFEP strain was grown in culture media with sulfate concentrations ranging from 0 to 23.92 mM, in absence and presence of Cr(VI) [1.7 and 3.3 mM]. In absence of Cr(VI), the yeast specific growth rate was practically the same in every sulfate concentration tested, which suggests that sulfate had no stimulating or inhibiting effect on the yeast cell growth. In contrast, at the two initial Cr(VI) concentrations assayed, the specific growth rate of Candida sp. FGSFEP rose when sulfate concentration increased. Likewise, the greater efficiencies and volumetric rates of Cr(VI) reduction exhibited by Candida sp. FGSFEP were obtained at high sulfate concentrations. Yeast was capable of reducing 100% of 1.7 mM Cr(VI) and 84% of 3.3 mM Cr(VI), with rates of 0.98 and 0.44 mg Cr(VI)/L h, with 10 and 23.92 mM sulfate concentrations, respectively. These results indicate that sulfate plays an important role in the tolerance to Cr(VI) and Cr(VI) reduction in Candida sp. FGSFEP. These findings may have significant implications in the biological treatment of Cr(VI)-laden wastewaters.
KW - Candida
KW - Cr(VI) reduction
KW - Hexavalent chromium [Cr(VI)]
KW - Modulation
KW - Sulfate
UR - http://www.scopus.com/inward/record.url?scp=53949120220&partnerID=8YFLogxK
U2 - 10.1007/s10295-008-0425-7
DO - 10.1007/s10295-008-0425-7
M3 - Artículo
C2 - 18712548
AN - SCOPUS:53949120220
SN - 1367-5435
VL - 35
SP - 1277
EP - 1287
JO - Journal of Industrial Microbiology and Biotechnology
JF - Journal of Industrial Microbiology and Biotechnology
IS - 11
ER -