Modulation of the release of adenosine by glucose and chemical hypoxia in cultured renal cells (MDCK line)

José L. Reyes, Estela Meléndez, Jorge Suárez, Marta Rangel, Marta Franco, Flavio Martinez

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

The effect of changes in the external concentrations of glucose on the release of adenosine of cultured renal cells (MDCK line) was studied. Adenosine release was markedly increased by the addition of glucose (5.5 mM) to monolayers of MDCK cells deprived of glucose for 24 hours. Addition of glucose to either the apical or basolateral side of the monolayer elicited a marked release of adenosine at the contralateral compartment. Removal of sodium in the external media blunted the response to glucose. Methylglucose that is transported into the cell but not metabolized also markedly increased adenosine release. Deoxyglucose that induces chemical hypoxia stimulated the release of adenosine. These results suggest that variations in extracellular glucose might be a physiological modulator of the cell regulation of adenosine. © 1995 Academic Press, Inc.
Original languageAmerican English
Pages (from-to)970-977
Number of pages872
JournalBiochemical and Biophysical Research Communications
DOIs
StatePublished - 1 Jan 1995

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adenosines
hypoxia
cultured cells
glucose
Adenosine
Glucose
Cultured Cells
Modulation
Kidney
modulation
Cell Line
Monolayers
cells
Madin Darby Canine Kidney Cells
Deoxyglucose
compartments
Modulators
Hypoxia
modulators
Sodium

Cite this

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abstract = "The effect of changes in the external concentrations of glucose on the release of adenosine of cultured renal cells (MDCK line) was studied. Adenosine release was markedly increased by the addition of glucose (5.5 mM) to monolayers of MDCK cells deprived of glucose for 24 hours. Addition of glucose to either the apical or basolateral side of the monolayer elicited a marked release of adenosine at the contralateral compartment. Removal of sodium in the external media blunted the response to glucose. Methylglucose that is transported into the cell but not metabolized also markedly increased adenosine release. Deoxyglucose that induces chemical hypoxia stimulated the release of adenosine. These results suggest that variations in extracellular glucose might be a physiological modulator of the cell regulation of adenosine. {\circledC} 1995 Academic Press, Inc.",
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Modulation of the release of adenosine by glucose and chemical hypoxia in cultured renal cells (MDCK line). / Reyes, José L.; Meléndez, Estela; Suárez, Jorge; Rangel, Marta; Franco, Marta; Martinez, Flavio.

In: Biochemical and Biophysical Research Communications, 01.01.1995, p. 970-977.

Research output: Contribution to journalArticle

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T1 - Modulation of the release of adenosine by glucose and chemical hypoxia in cultured renal cells (MDCK line)

AU - Reyes, José L.

AU - Meléndez, Estela

AU - Suárez, Jorge

AU - Rangel, Marta

AU - Franco, Marta

AU - Martinez, Flavio

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