Modulation by bovine lactoferrin of parameters associated with the IgA response in the proximal and distal small intestine of BALB/c mice

Marycarmen Godínez-Victoria, Teresita Rocío Cruz-Hernández, Humberto Reyna-Garfias, Reyna Elizabeth Barbosa-Cabrera, Maria Elisa Drago-Serrano, María Cristina Sánchez-Gómez, Rafael Campos-Rodríguez

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

© 2017 Informa UK Limited, trading as Taylor & Francis Group. Background: Secretory IgA (SIgA) and the polymeric immunoglobulin receptor (pIgR) have a pivotal role in gut homeostasis. Bovine lactoferrin (bLf) has been shown to modulate intestinal immunity and endogenous corticosterone. Considering the regionalization of the intestinal immune response, the aim of this work was to compare the impact of bLf on the IgA response in the proximal versus distal small intestine under physiological conditions. Methods: Groups of healthy male BALB/c mice were orally treated with one daily dose of bLf (50, 500, or 5000 μg) or untreated (control) for 7 d, and then sacrificed. From plasma samples, corticosterone levels were determined by an enzyme-linked immunosorbent assay (ELISA) kit. From distal and proximal segments of the small intestine, the following material was obtained: intestinal secretions to evaluate IgA levels by ELISA; epithelial cell extracts for protein-analysis of α-chain and pIgR by Western blot; mucosa samples for mRNA analysis of α-/J-chain, pIgR, and interleukin (IL)-2, -4, -5, and -6 by real-time quantitative reverse transcription polymerase chain reaction (RT-PCR). Results: With 5000 μg of bLf, there were greater modulatory effects in the distal (versus proximal) segment, evidenced by an increase in the (i) level of total and specific IgA, (ii) protein expression of α-chain and pIgR, (iii) mRNA transcripts of α-chain, IL-2 and IL-5, and (iv) level of plasma corticosterone. Conclusions: Endogenous corticosterone elicited by bLf may have allowed for an IL profile that favored the IgA antibody response. The latter has a key role in maintaining intestinal homeostasis.
Original languageAmerican English
Pages (from-to)66-73
Number of pages58
JournalImmunopharmacology and Immunotoxicology
DOIs
StatePublished - 4 Mar 2017

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Polymeric Immunoglobulin Receptors
Lactoferrin
Immunoglobulin A
Small Intestine
Corticosterone
Modulation
Immunosorbents
Interleukin-2
Assays
Homeostasis
Enzyme-Linked Immunosorbent Assay
Intestinal Secretions
Plasmas
Secretory Immunoglobulin A
Messenger RNA
Interleukins
Polymerase chain reaction
Interleukin-5
Enzymes
Transcription

Cite this

@article{36eaf40820ef4610817da1b829aacb63,
title = "Modulation by bovine lactoferrin of parameters associated with the IgA response in the proximal and distal small intestine of BALB/c mice",
abstract = "{\circledC} 2017 Informa UK Limited, trading as Taylor & Francis Group. Background: Secretory IgA (SIgA) and the polymeric immunoglobulin receptor (pIgR) have a pivotal role in gut homeostasis. Bovine lactoferrin (bLf) has been shown to modulate intestinal immunity and endogenous corticosterone. Considering the regionalization of the intestinal immune response, the aim of this work was to compare the impact of bLf on the IgA response in the proximal versus distal small intestine under physiological conditions. Methods: Groups of healthy male BALB/c mice were orally treated with one daily dose of bLf (50, 500, or 5000 μg) or untreated (control) for 7 d, and then sacrificed. From plasma samples, corticosterone levels were determined by an enzyme-linked immunosorbent assay (ELISA) kit. From distal and proximal segments of the small intestine, the following material was obtained: intestinal secretions to evaluate IgA levels by ELISA; epithelial cell extracts for protein-analysis of α-chain and pIgR by Western blot; mucosa samples for mRNA analysis of α-/J-chain, pIgR, and interleukin (IL)-2, -4, -5, and -6 by real-time quantitative reverse transcription polymerase chain reaction (RT-PCR). Results: With 5000 μg of bLf, there were greater modulatory effects in the distal (versus proximal) segment, evidenced by an increase in the (i) level of total and specific IgA, (ii) protein expression of α-chain and pIgR, (iii) mRNA transcripts of α-chain, IL-2 and IL-5, and (iv) level of plasma corticosterone. Conclusions: Endogenous corticosterone elicited by bLf may have allowed for an IL profile that favored the IgA antibody response. The latter has a key role in maintaining intestinal homeostasis.",
author = "Marycarmen God{\'i}nez-Victoria and Cruz-Hern{\'a}ndez, {Teresita Roc{\'i}o} and Humberto Reyna-Garfias and Barbosa-Cabrera, {Reyna Elizabeth} and Drago-Serrano, {Maria Elisa} and S{\'a}nchez-G{\'o}mez, {Mar{\'i}a Cristina} and Rafael Campos-Rodr{\'i}guez",
year = "2017",
month = "3",
day = "4",
doi = "10.1080/08923973.2017.1282513",
language = "American English",
pages = "66--73",
journal = "Immunopharmacology and Immunotoxicology",
issn = "0892-3973",
publisher = "Informa Healthcare",

}

Modulation by bovine lactoferrin of parameters associated with the IgA response in the proximal and distal small intestine of BALB/c mice. / Godínez-Victoria, Marycarmen; Cruz-Hernández, Teresita Rocío; Reyna-Garfias, Humberto; Barbosa-Cabrera, Reyna Elizabeth; Drago-Serrano, Maria Elisa; Sánchez-Gómez, María Cristina; Campos-Rodríguez, Rafael.

In: Immunopharmacology and Immunotoxicology, 04.03.2017, p. 66-73.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Modulation by bovine lactoferrin of parameters associated with the IgA response in the proximal and distal small intestine of BALB/c mice

AU - Godínez-Victoria, Marycarmen

AU - Cruz-Hernández, Teresita Rocío

AU - Reyna-Garfias, Humberto

AU - Barbosa-Cabrera, Reyna Elizabeth

AU - Drago-Serrano, Maria Elisa

AU - Sánchez-Gómez, María Cristina

AU - Campos-Rodríguez, Rafael

PY - 2017/3/4

Y1 - 2017/3/4

N2 - © 2017 Informa UK Limited, trading as Taylor & Francis Group. Background: Secretory IgA (SIgA) and the polymeric immunoglobulin receptor (pIgR) have a pivotal role in gut homeostasis. Bovine lactoferrin (bLf) has been shown to modulate intestinal immunity and endogenous corticosterone. Considering the regionalization of the intestinal immune response, the aim of this work was to compare the impact of bLf on the IgA response in the proximal versus distal small intestine under physiological conditions. Methods: Groups of healthy male BALB/c mice were orally treated with one daily dose of bLf (50, 500, or 5000 μg) or untreated (control) for 7 d, and then sacrificed. From plasma samples, corticosterone levels were determined by an enzyme-linked immunosorbent assay (ELISA) kit. From distal and proximal segments of the small intestine, the following material was obtained: intestinal secretions to evaluate IgA levels by ELISA; epithelial cell extracts for protein-analysis of α-chain and pIgR by Western blot; mucosa samples for mRNA analysis of α-/J-chain, pIgR, and interleukin (IL)-2, -4, -5, and -6 by real-time quantitative reverse transcription polymerase chain reaction (RT-PCR). Results: With 5000 μg of bLf, there were greater modulatory effects in the distal (versus proximal) segment, evidenced by an increase in the (i) level of total and specific IgA, (ii) protein expression of α-chain and pIgR, (iii) mRNA transcripts of α-chain, IL-2 and IL-5, and (iv) level of plasma corticosterone. Conclusions: Endogenous corticosterone elicited by bLf may have allowed for an IL profile that favored the IgA antibody response. The latter has a key role in maintaining intestinal homeostasis.

AB - © 2017 Informa UK Limited, trading as Taylor & Francis Group. Background: Secretory IgA (SIgA) and the polymeric immunoglobulin receptor (pIgR) have a pivotal role in gut homeostasis. Bovine lactoferrin (bLf) has been shown to modulate intestinal immunity and endogenous corticosterone. Considering the regionalization of the intestinal immune response, the aim of this work was to compare the impact of bLf on the IgA response in the proximal versus distal small intestine under physiological conditions. Methods: Groups of healthy male BALB/c mice were orally treated with one daily dose of bLf (50, 500, or 5000 μg) or untreated (control) for 7 d, and then sacrificed. From plasma samples, corticosterone levels were determined by an enzyme-linked immunosorbent assay (ELISA) kit. From distal and proximal segments of the small intestine, the following material was obtained: intestinal secretions to evaluate IgA levels by ELISA; epithelial cell extracts for protein-analysis of α-chain and pIgR by Western blot; mucosa samples for mRNA analysis of α-/J-chain, pIgR, and interleukin (IL)-2, -4, -5, and -6 by real-time quantitative reverse transcription polymerase chain reaction (RT-PCR). Results: With 5000 μg of bLf, there were greater modulatory effects in the distal (versus proximal) segment, evidenced by an increase in the (i) level of total and specific IgA, (ii) protein expression of α-chain and pIgR, (iii) mRNA transcripts of α-chain, IL-2 and IL-5, and (iv) level of plasma corticosterone. Conclusions: Endogenous corticosterone elicited by bLf may have allowed for an IL profile that favored the IgA antibody response. The latter has a key role in maintaining intestinal homeostasis.

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U2 - 10.1080/08923973.2017.1282513

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