TY - JOUR
T1 - Moderate aerobic exercise on bone quality changes associated with aging and oxidative stress in BALB/c mice
AU - Sierra-Ramírez, José Alfredo
AU - Saucedo-Bueno, Lourdes
AU - García-Hernández, Ana Lilia
AU - Martínez-Dávalos, Arnulfo
AU - Rodríguez-López, Camilo
AU - Drago-Serrano, Maria Elisa
AU - Godínez-Victoria, Marycarmen
N1 - Publisher Copyright:
© 2022
PY - 2022/4
Y1 - 2022/4
N2 - Exercise encourages active and healthy aging, maintaining functional and physical capabilities. This study aimed to assess the effects of a long-term moderate aerobic exercise protocol on bone microarchitecture and fragility associated with chronic inflammation and oxidative stress in aging. Male BALB/c mice (n = 10 per group) underwent a moderate exercise protocol from 13 weeks to 27 (adulthood age) or 108 weeks of age (elderly age) and were then sacrificed. Age-match sedentary mice were included as a control group. Serum cortisol concentrations were determined by chemiluminescent immunoassay, C-reactive protein (CRP) by a turbidimetric assay, advanced glycation end-products (AGEs) and malondialdehyde (MDA) by fluorescent spectroscopy, and total glutathione (GSH) by colorimetric method. The right femur was dissected for morphometric and densitometric analysis by computerized microtomography (µCT), and biomechanical properties were assessed using a three-point bending device. Muscle from the same extremity was obtained to determine relative mRNA expression of pro-inflammatory cytokines (TNF-α and IL-6) by RT-qPCR. Statistical differences were evaluated by two-way ANOVA and Holm-Sidak method post hoc with P < 0.05. In elderly mice, moderate exercise increased glutathione levels and microarchitecture complexity but decreased bone fragility and oxidative stress markers, cortisol, and pro-inflammatory cytokines. In conclusion, these results suggest a strong link between a pro-inflammatory state and age-conditioned oxidative stress on bone quality. Thus, on a human scale, moderate aerobic exercise may improve bone quality during aging.
AB - Exercise encourages active and healthy aging, maintaining functional and physical capabilities. This study aimed to assess the effects of a long-term moderate aerobic exercise protocol on bone microarchitecture and fragility associated with chronic inflammation and oxidative stress in aging. Male BALB/c mice (n = 10 per group) underwent a moderate exercise protocol from 13 weeks to 27 (adulthood age) or 108 weeks of age (elderly age) and were then sacrificed. Age-match sedentary mice were included as a control group. Serum cortisol concentrations were determined by chemiluminescent immunoassay, C-reactive protein (CRP) by a turbidimetric assay, advanced glycation end-products (AGEs) and malondialdehyde (MDA) by fluorescent spectroscopy, and total glutathione (GSH) by colorimetric method. The right femur was dissected for morphometric and densitometric analysis by computerized microtomography (µCT), and biomechanical properties were assessed using a three-point bending device. Muscle from the same extremity was obtained to determine relative mRNA expression of pro-inflammatory cytokines (TNF-α and IL-6) by RT-qPCR. Statistical differences were evaluated by two-way ANOVA and Holm-Sidak method post hoc with P < 0.05. In elderly mice, moderate exercise increased glutathione levels and microarchitecture complexity but decreased bone fragility and oxidative stress markers, cortisol, and pro-inflammatory cytokines. In conclusion, these results suggest a strong link between a pro-inflammatory state and age-conditioned oxidative stress on bone quality. Thus, on a human scale, moderate aerobic exercise may improve bone quality during aging.
KW - Aging
KW - Inflammation
KW - Moderate exercise
KW - Oxide-reduction system
KW - Quality bone
UR - http://www.scopus.com/inward/record.url?scp=85126320589&partnerID=8YFLogxK
U2 - 10.1016/j.jbiomech.2022.111035
DO - 10.1016/j.jbiomech.2022.111035
M3 - Artículo
C2 - 35298960
AN - SCOPUS:85126320589
SN - 0021-9290
VL - 135
JO - Journal of Biomechanics
JF - Journal of Biomechanics
M1 - 111035
ER -