TY - JOUR
T1 - Microvascular protective activity of anthocyanosides-rich extract from Acalypha langiana
AU - Gutierrez, Rosa Martha Perez
AU - Solis, Rosario Vargas
PY - 2008
Y1 - 2008
N2 - A mixture of anthocyanosides from the leaves of Acalypha langiana consisting of cyanidin-3-glucoside, cyanidin-3-rutinoside, peonidin-3-galactoside, petunidin-3-glucoside, delphinidin-3-glucoside, malvidin-3-glucoside, malvidin-5,5-diglucoside and the anthocyanidin pelargonidin, were characterised by means of HPLC and UV-VIS. Phenolic components were extracted with pure methanol. The samples were diluted in the mixture of methanol and formic acid just before the injection on the column and separated on Hypersil PEP 300 C18 chromatographic column using gradient solvent system consisting of formic acid, water and methanol. DAD detector was employed at 520 nm for anthocyanins. An Acalypha langiona anthocyanosides preparation (equivalent to 30% of anthocyanidins) demonstrated significant vasoprotective in rabbits, the skin capillary permeability increase, due to chloroform, was reduced after i.p. (25-100 mg/kg) anthocyanosides. Comparison was made between the action of the methanol extract and a known protective microvascular drug as troxerutin (25 mg/kg). The mixed of anthocyanosides was more active that the troxerutin.
AB - A mixture of anthocyanosides from the leaves of Acalypha langiana consisting of cyanidin-3-glucoside, cyanidin-3-rutinoside, peonidin-3-galactoside, petunidin-3-glucoside, delphinidin-3-glucoside, malvidin-3-glucoside, malvidin-5,5-diglucoside and the anthocyanidin pelargonidin, were characterised by means of HPLC and UV-VIS. Phenolic components were extracted with pure methanol. The samples were diluted in the mixture of methanol and formic acid just before the injection on the column and separated on Hypersil PEP 300 C18 chromatographic column using gradient solvent system consisting of formic acid, water and methanol. DAD detector was employed at 520 nm for anthocyanins. An Acalypha langiona anthocyanosides preparation (equivalent to 30% of anthocyanidins) demonstrated significant vasoprotective in rabbits, the skin capillary permeability increase, due to chloroform, was reduced after i.p. (25-100 mg/kg) anthocyanosides. Comparison was made between the action of the methanol extract and a known protective microvascular drug as troxerutin (25 mg/kg). The mixed of anthocyanosides was more active that the troxerutin.
KW - Acalypha langiana
KW - Anthocyanidins
KW - Vasoprotective activity
UR - http://www.scopus.com/inward/record.url?scp=69749124812&partnerID=8YFLogxK
M3 - Artículo
SN - 0973-1296
VL - 4
SP - 85
EP - 88
JO - Pharmacognosy Magazine
JF - Pharmacognosy Magazine
IS - 14
ER -