Microvascular protective activity of anthocyanosides-rich extract from Acalypha langiana

Rosa Martha Perez Gutierrez, Rosario Vargas Solis

Research output: Contribution to journalArticleResearchpeer-review

Abstract

A mixture of anthocyanosides from the leaves of Acalypha langiana consisting of cyanidin-3-glucoside, cyanidin-3-rutinoside, peonidin-3-galactoside, petunidin-3-glucoside, delphinidin-3-glucoside, malvidin-3-glucoside, malvidin-5,5-diglucoside and the anthocyanidin pelargonidin, were characterised by means of HPLC and UV-VIS. Phenolic components were extracted with pure methanol. The samples were diluted in the mixture of methanol and formic acid just before the injection on the column and separated on Hypersil PEP 300 C18 chromatographic column using gradient solvent system consisting of formic acid, water and methanol. DAD detector was employed at 520 nm for anthocyanins. An Acalypha langiona anthocyanosides preparation (equivalent to 30% of anthocyanidins) demonstrated significant vasoprotective in rabbits, the skin capillary permeability increase, due to chloroform, was reduced after i.p. (25-100 mg/kg) anthocyanosides. Comparison was made between the action of the methanol extract and a known protective microvascular drug as troxerutin (25 mg/kg). The mixed of anthocyanosides was more active that the troxerutin.
Original languageAmerican English
Pages (from-to)85-88
Number of pages76
JournalPharmacognosy Magazine
StatePublished - 18 Aug 2008

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formic acid
Methanol
Anthocyanins
Formic acid
Protective Agents
Galactosides
Capillary Permeability
Chloroform
Chlorine compounds
Skin
High Pressure Liquid Chromatography
Rabbits
Detectors
Injections
Acalypha
Glucosides
Water
troxerutin

Cite this

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title = "Microvascular protective activity of anthocyanosides-rich extract from Acalypha langiana",
abstract = "A mixture of anthocyanosides from the leaves of Acalypha langiana consisting of cyanidin-3-glucoside, cyanidin-3-rutinoside, peonidin-3-galactoside, petunidin-3-glucoside, delphinidin-3-glucoside, malvidin-3-glucoside, malvidin-5,5-diglucoside and the anthocyanidin pelargonidin, were characterised by means of HPLC and UV-VIS. Phenolic components were extracted with pure methanol. The samples were diluted in the mixture of methanol and formic acid just before the injection on the column and separated on Hypersil PEP 300 C18 chromatographic column using gradient solvent system consisting of formic acid, water and methanol. DAD detector was employed at 520 nm for anthocyanins. An Acalypha langiona anthocyanosides preparation (equivalent to 30{\%} of anthocyanidins) demonstrated significant vasoprotective in rabbits, the skin capillary permeability increase, due to chloroform, was reduced after i.p. (25-100 mg/kg) anthocyanosides. Comparison was made between the action of the methanol extract and a known protective microvascular drug as troxerutin (25 mg/kg). The mixed of anthocyanosides was more active that the troxerutin.",
author = "Gutierrez, {Rosa Martha Perez} and Solis, {Rosario Vargas}",
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journal = "Pharmacognosy Magazine",
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Microvascular protective activity of anthocyanosides-rich extract from Acalypha langiana. / Gutierrez, Rosa Martha Perez; Solis, Rosario Vargas.

In: Pharmacognosy Magazine, 18.08.2008, p. 85-88.

Research output: Contribution to journalArticleResearchpeer-review

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