Microbiologic, serologic diagnosis, and genotypification of Helicobacter pylori isolated from biopsies in children and adult people. Molecular detection of the cag pathogenicity island of Helicobacter pylori

Silvia Giono Cerezo, Margarita Camorlinga Ponce, Germán Rubén Aguilar Gutiérrez

Research output: Contribution to conferencePaper

1 Citation (Scopus)

Abstract

Hp diagnostic is made by invasive methods using gastric biopsy of antrum, for culture and histological study. Non invasive are serology and urea breath test. 152 Hp from 19 children's with acute gastritis (46. 1%); 9 Hp from an adult. There had ampicillin resistance, 27. 4%, claritromicin 21. 8%, metronidazol 58. 4% and tetracycline 31. 5%, the 21 % susceptible to four antimicrobial. RAPD-PCR with 4 primers gave 44 profiles, related with clinical profile. In 7 children, there were two profiles RAPD. Three were similar but different each other. AFLP 23 adults 151 Hp; cagA y vacA genotypes gave unique patterns for each patient. The genes cagA, babA and oipA, secuencing and compared with reported Hp (Genbank); gave high polymorfism. Everything indicates that there are colonization with multiple Hp strains in Mexicans. In 645 sera from 352 children: 36.9% with Hp, the 46. 9% gave him/her anti-shits and 16. 2% antibodies to urease. Adults 293 (89. 1%) with Hp, 78. 9% gave anti-CagA and 59% antibodies to urease. The expression of IL-8 was bigger in infected children that in not infected and more significant in peptic ulcer. Genomic library Cag-PAI revealed 90% (Hp) positive, they had the complete PAI, 2 were incomplete and three negatives. PCR -LiPA LiPA (LineProbe Assay) is suggestive for genotyping assays.
Original languageAmerican English
Pages99-104
Number of pages88
StatePublished - 1 Apr 2006
EventRevista Latinoamericana de Microbiologia -
Duration: 1 Apr 2006 → …

Conference

ConferenceRevista Latinoamericana de Microbiologia
Period1/04/06 → …

Fingerprint

Genomic Islands
Helicobacter pylori
Biopsy
Urease
Plasminogen Activator Inhibitor 2
Random Amplified Polymorphic DNA Technique
Ampicillin Resistance
Pyloric Antrum
Breath Tests
Genomic Library
Antibodies
Nucleic Acid Databases
Gastritis
Serology
Tetracycline
Peptic Ulcer
Interleukin-8
Urea
Genotype
Polymerase Chain Reaction

Cite this

@conference{6dd27b432d844744892afd3498e9b35e,
title = "Microbiologic, serologic diagnosis, and genotypification of Helicobacter pylori isolated from biopsies in children and adult people. Molecular detection of the cag pathogenicity island of Helicobacter pylori",
abstract = "Hp diagnostic is made by invasive methods using gastric biopsy of antrum, for culture and histological study. Non invasive are serology and urea breath test. 152 Hp from 19 children's with acute gastritis (46. 1{\%}); 9 Hp from an adult. There had ampicillin resistance, 27. 4{\%}, claritromicin 21. 8{\%}, metronidazol 58. 4{\%} and tetracycline 31. 5{\%}, the 21 {\%} susceptible to four antimicrobial. RAPD-PCR with 4 primers gave 44 profiles, related with clinical profile. In 7 children, there were two profiles RAPD. Three were similar but different each other. AFLP 23 adults 151 Hp; cagA y vacA genotypes gave unique patterns for each patient. The genes cagA, babA and oipA, secuencing and compared with reported Hp (Genbank); gave high polymorfism. Everything indicates that there are colonization with multiple Hp strains in Mexicans. In 645 sera from 352 children: 36.9{\%} with Hp, the 46. 9{\%} gave him/her anti-shits and 16. 2{\%} antibodies to urease. Adults 293 (89. 1{\%}) with Hp, 78. 9{\%} gave anti-CagA and 59{\%} antibodies to urease. The expression of IL-8 was bigger in infected children that in not infected and more significant in peptic ulcer. Genomic library Cag-PAI revealed 90{\%} (Hp) positive, they had the complete PAI, 2 were incomplete and three negatives. PCR -LiPA LiPA (LineProbe Assay) is suggestive for genotyping assays.",
author = "Cerezo, {Silvia Giono} and Ponce, {Margarita Camorlinga} and Guti{\'e}rrez, {Germ{\'a}n Rub{\'e}n Aguilar}",
year = "2006",
month = "4",
day = "1",
language = "American English",
pages = "99--104",
note = "Revista Latinoamericana de Microbiologia ; Conference date: 01-04-2006",

}

Microbiologic, serologic diagnosis, and genotypification of Helicobacter pylori isolated from biopsies in children and adult people. Molecular detection of the cag pathogenicity island of Helicobacter pylori. / Cerezo, Silvia Giono; Ponce, Margarita Camorlinga; Gutiérrez, Germán Rubén Aguilar.

2006. 99-104 Paper presented at Revista Latinoamericana de Microbiologia, .

Research output: Contribution to conferencePaper

TY - CONF

T1 - Microbiologic, serologic diagnosis, and genotypification of Helicobacter pylori isolated from biopsies in children and adult people. Molecular detection of the cag pathogenicity island of Helicobacter pylori

AU - Cerezo, Silvia Giono

AU - Ponce, Margarita Camorlinga

AU - Gutiérrez, Germán Rubén Aguilar

PY - 2006/4/1

Y1 - 2006/4/1

N2 - Hp diagnostic is made by invasive methods using gastric biopsy of antrum, for culture and histological study. Non invasive are serology and urea breath test. 152 Hp from 19 children's with acute gastritis (46. 1%); 9 Hp from an adult. There had ampicillin resistance, 27. 4%, claritromicin 21. 8%, metronidazol 58. 4% and tetracycline 31. 5%, the 21 % susceptible to four antimicrobial. RAPD-PCR with 4 primers gave 44 profiles, related with clinical profile. In 7 children, there were two profiles RAPD. Three were similar but different each other. AFLP 23 adults 151 Hp; cagA y vacA genotypes gave unique patterns for each patient. The genes cagA, babA and oipA, secuencing and compared with reported Hp (Genbank); gave high polymorfism. Everything indicates that there are colonization with multiple Hp strains in Mexicans. In 645 sera from 352 children: 36.9% with Hp, the 46. 9% gave him/her anti-shits and 16. 2% antibodies to urease. Adults 293 (89. 1%) with Hp, 78. 9% gave anti-CagA and 59% antibodies to urease. The expression of IL-8 was bigger in infected children that in not infected and more significant in peptic ulcer. Genomic library Cag-PAI revealed 90% (Hp) positive, they had the complete PAI, 2 were incomplete and three negatives. PCR -LiPA LiPA (LineProbe Assay) is suggestive for genotyping assays.

AB - Hp diagnostic is made by invasive methods using gastric biopsy of antrum, for culture and histological study. Non invasive are serology and urea breath test. 152 Hp from 19 children's with acute gastritis (46. 1%); 9 Hp from an adult. There had ampicillin resistance, 27. 4%, claritromicin 21. 8%, metronidazol 58. 4% and tetracycline 31. 5%, the 21 % susceptible to four antimicrobial. RAPD-PCR with 4 primers gave 44 profiles, related with clinical profile. In 7 children, there were two profiles RAPD. Three were similar but different each other. AFLP 23 adults 151 Hp; cagA y vacA genotypes gave unique patterns for each patient. The genes cagA, babA and oipA, secuencing and compared with reported Hp (Genbank); gave high polymorfism. Everything indicates that there are colonization with multiple Hp strains in Mexicans. In 645 sera from 352 children: 36.9% with Hp, the 46. 9% gave him/her anti-shits and 16. 2% antibodies to urease. Adults 293 (89. 1%) with Hp, 78. 9% gave anti-CagA and 59% antibodies to urease. The expression of IL-8 was bigger in infected children that in not infected and more significant in peptic ulcer. Genomic library Cag-PAI revealed 90% (Hp) positive, they had the complete PAI, 2 were incomplete and three negatives. PCR -LiPA LiPA (LineProbe Assay) is suggestive for genotyping assays.

UR - https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=34548046267&origin=inward

UR - https://www.scopus.com/inward/citedby.uri?partnerID=HzOxMe3b&scp=34548046267&origin=inward

M3 - Paper

SP - 99

EP - 104

ER -