Methods for analysis of time-resolved fluorescence data

E. Moreno-García, J. M. De La Rosa-Vázquez, S. Stolik-Isakina, P. Reyes-López

Research output: Contribution to conferencePaperpeer-review

1 Scopus citations

Abstract

We report the results of applying several deconvolution methods to estimate fluorescence decay curves and fluorescence lifetimes. The methods are applied to time-resolved fluorescence signals measured with a homemade spectrofluorometer. We measured the fluorescence emitted by three substances with known lifetimes: fluoresceine (4.03 ns), rhodamine 6G (4.08 ns), and rhodamine B (1.68 ns). Also we measured the fluorescence emitted by three mixtures of two substances. We review three deconvolution methods: Fourier transform, least-squares iterative reconvolution, and Laguerre functions. to estimate the intrinsic fluorescence of the samples. A program was developed in LabView 7.1 to apply the three deconvolution methods from the observed fluorescence signals and the instrumental response signal. We conclude from data analysis results, that deconvolution by Laguerre expansion provides the best approximation of fluorescence lifetimes, producing an error less than 0.8% for substances, and less than 2.5% for mixtures.

Original languageEnglish
Pages317-322
Number of pages6
DOIs
StatePublished - 2012
Externally publishedYes
Event22nd Annual International Conference on Electronics, Communications and Computers, CONIELECOMP 2012 - Cholula, Mexico
Duration: 27 Feb 201229 Feb 2012

Conference

Conference22nd Annual International Conference on Electronics, Communications and Computers, CONIELECOMP 2012
Country/TerritoryMexico
CityCholula
Period27/02/1229/02/12

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