TY - JOUR
T1 - Mechanical production of pellets for the application of entomopathogenic nematodes
T2 - effect of pre-acclimation of Steinernema glaseri on its survival time and infectivity against Phyllophaga vetula
AU - Cortés-Martínez, Carlos Inocencio
AU - Lewis, Edwin E.
AU - Ruiz-Vega, Jaime
AU - Martínez-Gutiérrez, Gabino Alberto
N1 - Publisher Copyright:
© 2017 Informa UK Limited, trading as Taylor & Francis Group.
PY - 2017/8/3
Y1 - 2017/8/3
N2 - The low survival time and diminished infectivity by entomopathogenic nematodes (EPNs) from granular formulations limit their efficiency against agricultural insect pests. This study determined the benefit of pre-acclimating infective juveniles (IJs) of Steinernema glaseri (NJ-43 strain) on extending their mean survival time (STm) in diatomaceous earth (DE) pellets and increasing their infectivity against Phyllophaga vetula. The IJs were reared in Galleria mellonella larvae placed in Petri dishes containing plaster of Paris (PP) or modified White traps (WTs). Pelletisation was performed in a machine operating on the principle of laminar flow using DE Celite® 209. Pellets were stored at room temperature (23 ± 3°C) and high relative humidity (96–100%). IJs harvested from WTs between the 3rd and the 5th days after the onset of emergence were more infective on P. vetula and pre-acclimation of S. glaseri in PP increased significantly its STm in the pellets; from 23.1 to 34.5 days, compared with non-pre-acclimatised IJs from WTs. However, juveniles with or without pre-acclimation formulated in DE pellets failed to achieve significant control of P. vetula. These results are discussed in light of the relationship between EPN survival and host infection by EPNs with possible effects of the formulation in DE pellets.
AB - The low survival time and diminished infectivity by entomopathogenic nematodes (EPNs) from granular formulations limit their efficiency against agricultural insect pests. This study determined the benefit of pre-acclimating infective juveniles (IJs) of Steinernema glaseri (NJ-43 strain) on extending their mean survival time (STm) in diatomaceous earth (DE) pellets and increasing their infectivity against Phyllophaga vetula. The IJs were reared in Galleria mellonella larvae placed in Petri dishes containing plaster of Paris (PP) or modified White traps (WTs). Pelletisation was performed in a machine operating on the principle of laminar flow using DE Celite® 209. Pellets were stored at room temperature (23 ± 3°C) and high relative humidity (96–100%). IJs harvested from WTs between the 3rd and the 5th days after the onset of emergence were more infective on P. vetula and pre-acclimation of S. glaseri in PP increased significantly its STm in the pellets; from 23.1 to 34.5 days, compared with non-pre-acclimatised IJs from WTs. However, juveniles with or without pre-acclimation formulated in DE pellets failed to achieve significant control of P. vetula. These results are discussed in light of the relationship between EPN survival and host infection by EPNs with possible effects of the formulation in DE pellets.
KW - Infectivity
KW - granular formulation
KW - pelletiser
KW - plaster of Paris
KW - white grub
UR - http://www.scopus.com/inward/record.url?scp=85028527163&partnerID=8YFLogxK
U2 - 10.1080/09583157.2017.1366423
DO - 10.1080/09583157.2017.1366423
M3 - Artículo
SN - 0958-3157
VL - 27
SP - 940
EP - 951
JO - Biocontrol Science and Technology
JF - Biocontrol Science and Technology
IS - 8
ER -