TY - JOUR
T1 - Measurement of suppressor activity of T CD4+CD25+ T reg cells using bromodeoxyuridine incorporation assay
AU - Ávalos-Martínez, Claudia E.
AU - Rodríguez-Alba, Juan Carlos
AU - Berrón-Ruiz, Laura
AU - Romero-Ramírez, Hector
AU - Santos-Argumedo, Leopoldo
AU - Jiménez-Zamudio, Luis Antonio
AU - Domínguez-López, Maria Lilia
AU - Vega-López, Armando
AU - García-Latorre, Ethel
N1 - Funding Information:
C.E. Ávalos-Martínez is a DSc. student who received a doctoral scholarship from CONACyT, México. L. Jiménez-Zamudio, ML Domínguez-López, A.Vega-López and E. García-Latorre are fellows of COFAA and EDI-IPN. The work was done in part with grants from SIP-IPN and CONACyT, México.
PY - 2013
Y1 - 2013
N2 - The suppressor effect of T regulatory lymphocytes in co-cultures with T effector cells obtained by magnetic columns from healthy donors and activated by CD3/CD28 was measured by a proliferation assay using BrdU incorporation and an ELISA test. Tritiated thymidine incorporation was used as a reference since it is the gold standard for proliferation assays. Both methods were used simultaneously in the same samples in order to compare them. Correlation between them was statistically significant (p < 0.001). The purification using magnetic columns was very efficient since CD4+CD25+ cells were also FOXP3+ therefore; they were identified as suppressor T cells. The use of BrdU incorporation in suppression assays is an excellent method that avoids the use of radioactive contaminating materials.
AB - The suppressor effect of T regulatory lymphocytes in co-cultures with T effector cells obtained by magnetic columns from healthy donors and activated by CD3/CD28 was measured by a proliferation assay using BrdU incorporation and an ELISA test. Tritiated thymidine incorporation was used as a reference since it is the gold standard for proliferation assays. Both methods were used simultaneously in the same samples in order to compare them. Correlation between them was statistically significant (p < 0.001). The purification using magnetic columns was very efficient since CD4+CD25+ cells were also FOXP3+ therefore; they were identified as suppressor T cells. The use of BrdU incorporation in suppression assays is an excellent method that avoids the use of radioactive contaminating materials.
KW - BrdU incorporation
KW - Effector T cells
KW - Regulatory T cells
KW - Suppression by T reg cell
KW - Thymidine incorporation
UR - http://www.scopus.com/inward/record.url?scp=84875963690&partnerID=8YFLogxK
U2 - 10.3109/08820139.2013.773337
DO - 10.3109/08820139.2013.773337
M3 - Artículo
C2 - 23883202
SN - 0882-0139
VL - 42
SP - 369
EP - 381
JO - Immunological Investigations
JF - Immunological Investigations
IS - 4
ER -