Measurement of suppressor activity of T CD4+CD25+ T reg cells using bromodeoxyuridine incorporation assay

Claudia E. Ávalos-Martínez, Juan Carlos Rodríguez-Alba, Laura Berrón-Ruiz, Hector Romero-Ramírez, Leopoldo Santos-Argumedo, Luis Antonio Jiménez-Zamudio, Maria Lilia Domínguez-López, Armando Vega-López, Ethel García-Latorre

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The suppressor effect of T regulatory lymphocytes in co-cultures with T effector cells obtained by magnetic columns from healthy donors and activated by CD3/CD28 was measured by a proliferation assay using BrdU incorporation and an ELISA test. Tritiated thymidine incorporation was used as a reference since it is the gold standard for proliferation assays. Both methods were used simultaneously in the same samples in order to compare them. Correlation between them was statistically significant (p < 0.001). The purification using magnetic columns was very efficient since CD4+CD25+ cells were also FOXP3+ therefore; they were identified as suppressor T cells. The use of BrdU incorporation in suppression assays is an excellent method that avoids the use of radioactive contaminating materials. © 2013 Informa Healthcare USA, Inc.
Original languageAmerican English
Pages (from-to)369-381
Number of pages330
JournalImmunological Investigations
DOIs
StatePublished - 12 Apr 2013

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Bromodeoxyuridine
Regulatory T-Lymphocytes
Coculture Techniques
Thymidine
Enzyme-Linked Immunosorbent Assay
Delivery of Health Care
T-Lymphocytes

Cite this

Ávalos-Martínez, Claudia E. ; Rodríguez-Alba, Juan Carlos ; Berrón-Ruiz, Laura ; Romero-Ramírez, Hector ; Santos-Argumedo, Leopoldo ; Jiménez-Zamudio, Luis Antonio ; Domínguez-López, Maria Lilia ; Vega-López, Armando ; García-Latorre, Ethel. / Measurement of suppressor activity of T CD4+CD25+ T reg cells using bromodeoxyuridine incorporation assay. In: Immunological Investigations. 2013 ; pp. 369-381.
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Measurement of suppressor activity of T CD4+CD25+ T reg cells using bromodeoxyuridine incorporation assay. / Ávalos-Martínez, Claudia E.; Rodríguez-Alba, Juan Carlos; Berrón-Ruiz, Laura; Romero-Ramírez, Hector; Santos-Argumedo, Leopoldo; Jiménez-Zamudio, Luis Antonio; Domínguez-López, Maria Lilia; Vega-López, Armando; García-Latorre, Ethel.

In: Immunological Investigations, 12.04.2013, p. 369-381.

Research output: Contribution to journalArticle

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AU - Romero-Ramírez, Hector

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AU - Jiménez-Zamudio, Luis Antonio

AU - Domínguez-López, Maria Lilia

AU - Vega-López, Armando

AU - García-Latorre, Ethel

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