Mass production of Scenedesmus incrassatulus in 8 and 40 liter disposable polyethylene bags with different culture media

L. Ceballos-Pinto, F. Martinez-Jeronimo

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Abstract

The green microalga Scenedesmus incrassatulus was grown in three culture media (Bold Basal, Bristol and one prepared by dilution of a commercial fertilizer for violets), in 8- and 40-liter polyethylene bags. Population growth was measured by assessing cell density, protein content and chlorophyll concentration. No significant differences were detected for the growth rates estimated for the cultures in Bold Basal and Bristol media, whereas the commercial fertilizer was not a good option for reducing the cost of culture medium and results were inconsistent, probably related with a low quality control in the fertilizer manufacturing. Besides, microalgae grown in the medium with fertilizer did not grow as coenobia, and showed a notably intracellular vacuolated aspect. In general, the best outcomes were obtained in the cultures carried out in 8-liter Bold Basal medium. From our results we conclude that it will be more convenient to culture S. incrassatulus in only one step (8 or 40 litres), because the produced biomass will have a better quality, and the chances of a significant bacterial contamination will be lower.
Original languageAmerican English
Pages (from-to)109-119
Number of pages97
JournalRevista Latinoamericana de Microbiologia
StatePublished - 3 Aug 1995

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scenedesmus
Scenedesmus
fertilizers
culture media
Fertilizers
bags
Polyethylene
polyethylene
Culture Media
Polyethylenes
polyethylenes
Viola
Microalgae
bacterial contamination
Population Growth
chlorophylls
Chlorophyll
biomass
quality control
microalgae

Cite this

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title = "Mass production of Scenedesmus incrassatulus in 8 and 40 liter disposable polyethylene bags with different culture media",
abstract = "The green microalga Scenedesmus incrassatulus was grown in three culture media (Bold Basal, Bristol and one prepared by dilution of a commercial fertilizer for violets), in 8- and 40-liter polyethylene bags. Population growth was measured by assessing cell density, protein content and chlorophyll concentration. No significant differences were detected for the growth rates estimated for the cultures in Bold Basal and Bristol media, whereas the commercial fertilizer was not a good option for reducing the cost of culture medium and results were inconsistent, probably related with a low quality control in the fertilizer manufacturing. Besides, microalgae grown in the medium with fertilizer did not grow as coenobia, and showed a notably intracellular vacuolated aspect. In general, the best outcomes were obtained in the cultures carried out in 8-liter Bold Basal medium. From our results we conclude that it will be more convenient to culture S. incrassatulus in only one step (8 or 40 litres), because the produced biomass will have a better quality, and the chances of a significant bacterial contamination will be lower.",
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N2 - The green microalga Scenedesmus incrassatulus was grown in three culture media (Bold Basal, Bristol and one prepared by dilution of a commercial fertilizer for violets), in 8- and 40-liter polyethylene bags. Population growth was measured by assessing cell density, protein content and chlorophyll concentration. No significant differences were detected for the growth rates estimated for the cultures in Bold Basal and Bristol media, whereas the commercial fertilizer was not a good option for reducing the cost of culture medium and results were inconsistent, probably related with a low quality control in the fertilizer manufacturing. Besides, microalgae grown in the medium with fertilizer did not grow as coenobia, and showed a notably intracellular vacuolated aspect. In general, the best outcomes were obtained in the cultures carried out in 8-liter Bold Basal medium. From our results we conclude that it will be more convenient to culture S. incrassatulus in only one step (8 or 40 litres), because the produced biomass will have a better quality, and the chances of a significant bacterial contamination will be lower.

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