Magnetic extraction and purification of DNA from whole human blood using a γ-Fe<inf>2</inf>O<inf>3</inf>@Chitosan@Polyaniline hybrid nanocomposite

Bruna Gomes Maciel, Romário Justino da Silva, Alicia Elizabeth Chávez-Guajardo, Juan Carlos Medina-Llamas, José Jarib Alcaraz-Espinoza, Celso Pinto de Melo

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8 Scopus citations

Abstract

© 2018 Elsevier Ltd We examined the properties of the nanocomposite γ-Fe2O3@Chi@Pani as an adsorbent of deoxyribonucleic acid (DNA). As a model system, we used an aqueous solution of salmon sperm DNA, whose decreasing concentration was followed by monitoring the 260 nm UV–vis absorption. After adjusting the data collected to a Langmuir isotherm curve, we estimated the adsorption capacity (qe) of the nanocomposite as 49.5 mg/g. We also observed that the kinetic model of the DNA capture presents a mixed character, with both chemical mechanisms and intraparticle diffusion processes involved. When the MNC was used to extract the DNA from complex samples (human blood), a capture rate of 80 ng/μL was achieved, with the collected fraction exhibiting good quality, as evaluated by PCR analysis and electrophoresis assays. These results suggest that the γ-Fe2O3@Chi@Pani nanocomposite is a promising adsorbent for use in protocols for purification of DNA from complex samples.
Original languageAmerican English
Pages (from-to)100-108
Number of pages9
JournalCarbohydrate Polymers
DOIs
StatePublished - 1 Oct 2018

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