TY - JOUR
T1 - Larvicidal effect of Clinopodium macrostemum essential oil extracted by microwave-assisted hydrodistillation against Culex quinquefasciatus (Diptera: Culicidae)
AU - Rojas-Olivos, Alejandra
AU - Solano-Gómez, Rodolfo
AU - Granados-Echegoyen, Carlos
AU - Santiago-Santiago, Luis Alberto
AU - García-Dávila, Jorge
AU - Pérez-Pacheco, Rafael
AU - Lagunez-Rivera, Luicita
N1 - Publisher Copyright:
© 2018, Sociedade Brasileira de Medicina Tropical. All rights reserved.
PY - 2018/4/1
Y1 - 2018/4/1
N2 - Introduction: In the present study, we determined the chemical composition of Clinopodium macrostemum essential oil obtained by microwave-assisted hydrodistillation and its effect on the growth and development of Culex quinquefasciatus mosquito larvae. Methods: The essential oil compounds were identified by gas chromatography-mass spectrometry analysis, and bioassays were conducted to evaluate the influence on the developmental stages of early second instar larvae of Cx. quinquefasciatus until the emergence of adults, using essential oil concentrations of 50, 100, 200, 400, and 800mg/L. Results: The gas chromatographymass spectrometry analyses revealed that the leaf essential oil of C. macrostemum contained 32 compounds and the major chemical compounds identified were linalool (55.4%), nerol (6.4%), caryophyllene (6.25%), menthone (5.8%), geraniol acetate (4.1%), terpineol (3.7%), and pulegone (2.8%). The essential oil yield obtained by microwave-assisted hydrodistillation was 0.8% at 20 min. The treatments showed lethal concentrations (LC50 and LC90) of 22.49 and 833.35mg/L, respectively, after the final measurement of the total number of dead larvae (second, third, and fourth instars), and LC50 and LC90 of 6.62 and 693.35mg/L, respectively, at the end of the experimental period. The essential oil inhibited the growth and development of the mosquito larvae by 32% (relative growth index = 0.68) at 50mg/L, and up to 47% (relative growth index = 0.53) at 800mg/L. Conclusion: The present study demonstrated the larvicidal effect of C. macrostemum essential oil on Cx. quinquefasciatus, which can be attributed to the oxygenate compounds obtained by the extraction method.
AB - Introduction: In the present study, we determined the chemical composition of Clinopodium macrostemum essential oil obtained by microwave-assisted hydrodistillation and its effect on the growth and development of Culex quinquefasciatus mosquito larvae. Methods: The essential oil compounds were identified by gas chromatography-mass spectrometry analysis, and bioassays were conducted to evaluate the influence on the developmental stages of early second instar larvae of Cx. quinquefasciatus until the emergence of adults, using essential oil concentrations of 50, 100, 200, 400, and 800mg/L. Results: The gas chromatographymass spectrometry analyses revealed that the leaf essential oil of C. macrostemum contained 32 compounds and the major chemical compounds identified were linalool (55.4%), nerol (6.4%), caryophyllene (6.25%), menthone (5.8%), geraniol acetate (4.1%), terpineol (3.7%), and pulegone (2.8%). The essential oil yield obtained by microwave-assisted hydrodistillation was 0.8% at 20 min. The treatments showed lethal concentrations (LC50 and LC90) of 22.49 and 833.35mg/L, respectively, after the final measurement of the total number of dead larvae (second, third, and fourth instars), and LC50 and LC90 of 6.62 and 693.35mg/L, respectively, at the end of the experimental period. The essential oil inhibited the growth and development of the mosquito larvae by 32% (relative growth index = 0.68) at 50mg/L, and up to 47% (relative growth index = 0.53) at 800mg/L. Conclusion: The present study demonstrated the larvicidal effect of C. macrostemum essential oil on Cx. quinquefasciatus, which can be attributed to the oxygenate compounds obtained by the extraction method.
KW - Caryophyllene
KW - Filariasis
KW - Gas chromatography–mass spectrophotometry
KW - Linalool
KW - Medicinal plants
KW - Pennyroyal
UR - http://www.scopus.com/inward/record.url?scp=85049203164&partnerID=8YFLogxK
U2 - 10.1590/0037-8682-0284-2017
DO - 10.1590/0037-8682-0284-2017
M3 - Artículo
C2 - 29972558
AN - SCOPUS:85049203164
SN - 0037-8682
VL - 51
SP - 291
EP - 296
JO - Revista da Sociedade Brasileira de Medicina Tropical
JF - Revista da Sociedade Brasileira de Medicina Tropical
IS - 3
M1 - S0037-86822018000300291
ER -