Jasmonic acid stimulates the oxidative responses and triterpene production in Jatropha curcas cell suspension cultures through mevalonate as biosynthetic precursor

Fabiola Zaragoza-Martínez, Gonzalo G. Lucho-Constantino, Teresa Ponce-Noyola, Fernando Esparza-García, Héctor Poggi-Varaldo, Carlos M. Cerda-García-Rojas, Gabriela Trejo-Tapia, Ana C. Ramos-Valdivia

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Abstract

© 2016, Springer Science+Business Media Dordrecht. Jatropha curcas has considerable potential for production of biodiesel and secondary metabolites with medicinal uses. Herein, J. curcas cell suspension cultures were established to study the effect of jasmonic acid (JA) elicitation on triterpene production and oxidative responses. Cell cultures grown in dark conditions reached maximum biomass accumulation at the 12th day of culture (14.3 ± 0.45 g DW L−1) with a specific growth rate μ = 0.131 d−1. Elicitation with JA (200 or 400 μM) on 4-days-old cell cultures caused reduction in biomass and triterpene contents. In contrast, application of 200 μM JA at the 7th day of culture triggered triterpene accumulation by three times (1180 ± 12.3 μg g−1 DW, at day 2) with respect to control, without significant changes in biomass and viability. After 2 days of elicitation, betulin increased up to 7.3-fold (from 110.6 ± 20.7 to 808.7 ± 55.4 μg g−1 DW), while betulinic acid reached the maximum amount at day 6 after elicitation (245.6 ± 3.7 to 835 ± 41.5 μg g−1 DW). Lupeol presented a moderate increase (167.9 ± 51.0–288.8 ± 7.3 μg g−1 DW) along 8 days after elicitation. In correlation with triterpene production, JA application induced oxidative responses evaluated by an increase in the H2O2 levels up to three times and of malondialdehyde by 59 %. At day 4 after elicitation, catalase showed higher increase (122 %) than peroxidases (63 %) and ascorbate peroxidase (26 %). Incorporation of radioactive labels from (R,S)-[2-14C]mevalonic acid in triterpenes and sterols confirmed its role as metabolic precursor.
Original languageAmerican English
Pages (from-to)47-56
Number of pages41
JournalPlant Cell, Tissue and Organ Culture
DOIs
StatePublished - 1 Oct 2016

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Jatropha curcas
jasmonic acid
triterpenoids
cell suspension culture
cell culture
betulin
mevalonic acid
lupeol
peroxidases
biomass
biodiesel
ascorbate peroxidase
specific growth rate
malondialdehyde
sterols
secondary metabolites
biomass production
catalase
viability

Cite this

Zaragoza-Martínez, F., Lucho-Constantino, G. G., Ponce-Noyola, T., Esparza-García, F., Poggi-Varaldo, H., Cerda-García-Rojas, C. M., ... Ramos-Valdivia, A. C. (2016). Jasmonic acid stimulates the oxidative responses and triterpene production in Jatropha curcas cell suspension cultures through mevalonate as biosynthetic precursor. Plant Cell, Tissue and Organ Culture, 47-56. https://doi.org/10.1007/s11240-016-1028-z
Zaragoza-Martínez, Fabiola ; Lucho-Constantino, Gonzalo G. ; Ponce-Noyola, Teresa ; Esparza-García, Fernando ; Poggi-Varaldo, Héctor ; Cerda-García-Rojas, Carlos M. ; Trejo-Tapia, Gabriela ; Ramos-Valdivia, Ana C. / Jasmonic acid stimulates the oxidative responses and triterpene production in Jatropha curcas cell suspension cultures through mevalonate as biosynthetic precursor. In: Plant Cell, Tissue and Organ Culture. 2016 ; pp. 47-56.
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author = "Fabiola Zaragoza-Mart{\'i}nez and Lucho-Constantino, {Gonzalo G.} and Teresa Ponce-Noyola and Fernando Esparza-Garc{\'i}a and H{\'e}ctor Poggi-Varaldo and Cerda-Garc{\'i}a-Rojas, {Carlos M.} and Gabriela Trejo-Tapia and Ramos-Valdivia, {Ana C.}",
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Jasmonic acid stimulates the oxidative responses and triterpene production in Jatropha curcas cell suspension cultures through mevalonate as biosynthetic precursor. / Zaragoza-Martínez, Fabiola; Lucho-Constantino, Gonzalo G.; Ponce-Noyola, Teresa; Esparza-García, Fernando; Poggi-Varaldo, Héctor; Cerda-García-Rojas, Carlos M.; Trejo-Tapia, Gabriela; Ramos-Valdivia, Ana C.

In: Plant Cell, Tissue and Organ Culture, 01.10.2016, p. 47-56.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - Jasmonic acid stimulates the oxidative responses and triterpene production in Jatropha curcas cell suspension cultures through mevalonate as biosynthetic precursor

AU - Zaragoza-Martínez, Fabiola

AU - Lucho-Constantino, Gonzalo G.

AU - Ponce-Noyola, Teresa

AU - Esparza-García, Fernando

AU - Poggi-Varaldo, Héctor

AU - Cerda-García-Rojas, Carlos M.

AU - Trejo-Tapia, Gabriela

AU - Ramos-Valdivia, Ana C.

PY - 2016/10/1

Y1 - 2016/10/1

N2 - © 2016, Springer Science+Business Media Dordrecht. Jatropha curcas has considerable potential for production of biodiesel and secondary metabolites with medicinal uses. Herein, J. curcas cell suspension cultures were established to study the effect of jasmonic acid (JA) elicitation on triterpene production and oxidative responses. Cell cultures grown in dark conditions reached maximum biomass accumulation at the 12th day of culture (14.3 ± 0.45 g DW L−1) with a specific growth rate μ = 0.131 d−1. Elicitation with JA (200 or 400 μM) on 4-days-old cell cultures caused reduction in biomass and triterpene contents. In contrast, application of 200 μM JA at the 7th day of culture triggered triterpene accumulation by three times (1180 ± 12.3 μg g−1 DW, at day 2) with respect to control, without significant changes in biomass and viability. After 2 days of elicitation, betulin increased up to 7.3-fold (from 110.6 ± 20.7 to 808.7 ± 55.4 μg g−1 DW), while betulinic acid reached the maximum amount at day 6 after elicitation (245.6 ± 3.7 to 835 ± 41.5 μg g−1 DW). Lupeol presented a moderate increase (167.9 ± 51.0–288.8 ± 7.3 μg g−1 DW) along 8 days after elicitation. In correlation with triterpene production, JA application induced oxidative responses evaluated by an increase in the H2O2 levels up to three times and of malondialdehyde by 59 %. At day 4 after elicitation, catalase showed higher increase (122 %) than peroxidases (63 %) and ascorbate peroxidase (26 %). Incorporation of radioactive labels from (R,S)-[2-14C]mevalonic acid in triterpenes and sterols confirmed its role as metabolic precursor.

AB - © 2016, Springer Science+Business Media Dordrecht. Jatropha curcas has considerable potential for production of biodiesel and secondary metabolites with medicinal uses. Herein, J. curcas cell suspension cultures were established to study the effect of jasmonic acid (JA) elicitation on triterpene production and oxidative responses. Cell cultures grown in dark conditions reached maximum biomass accumulation at the 12th day of culture (14.3 ± 0.45 g DW L−1) with a specific growth rate μ = 0.131 d−1. Elicitation with JA (200 or 400 μM) on 4-days-old cell cultures caused reduction in biomass and triterpene contents. In contrast, application of 200 μM JA at the 7th day of culture triggered triterpene accumulation by three times (1180 ± 12.3 μg g−1 DW, at day 2) with respect to control, without significant changes in biomass and viability. After 2 days of elicitation, betulin increased up to 7.3-fold (from 110.6 ± 20.7 to 808.7 ± 55.4 μg g−1 DW), while betulinic acid reached the maximum amount at day 6 after elicitation (245.6 ± 3.7 to 835 ± 41.5 μg g−1 DW). Lupeol presented a moderate increase (167.9 ± 51.0–288.8 ± 7.3 μg g−1 DW) along 8 days after elicitation. In correlation with triterpene production, JA application induced oxidative responses evaluated by an increase in the H2O2 levels up to three times and of malondialdehyde by 59 %. At day 4 after elicitation, catalase showed higher increase (122 %) than peroxidases (63 %) and ascorbate peroxidase (26 %). Incorporation of radioactive labels from (R,S)-[2-14C]mevalonic acid in triterpenes and sterols confirmed its role as metabolic precursor.

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