TY - JOUR
T1 - Isotopic variation in blood components based on their biochemistry and physiology
T2 - A comparison between sharks and fur seals
AU - Tamburin, Elena
AU - Amador-Capitanachi, María José
AU - Galván-Magaña, Felipe
AU - Norris, Tenaya A.
AU - Elorriaga-Verplancken, Fernando R.
N1 - Publisher Copyright:
© 2019 Wiley Periodicals, Inc.
PY - 2020/2/1
Y1 - 2020/2/1
N2 - Research using stable isotopes analysis (SIA) of carbon (δ13C) and nitrogen (δ15N) in blood components is lacking, because of the challenge of sample collection, processing, and storage in remote areas. There also is a paucity of information regarding the effect of tissue biochemical composition on isotopic ratios with few comparisons among taxa. We collected blood samples from shortfin mako sharks (n = 70; 2016) and Guadalupe fur seals (n = 25; 2017). All samples were centrifuged to obtain plasma from sharks and serum from the Guadalupe fur seals, and all the samples were prepared for SIA and analyzed using a Costech 4010 elemental analyzer interfaced with a Delta V Plus isotope ratio mass spectrometer. We found significant differences between plasma δ13C values of shortfin mako sharks (−17.6 ± 0.9‰) and serum of Guadalupe fur seals (−20.3 ± 1.2‰), but we did not find any differences for δ15N values between the two species. The differences in δ13C values between species are probably due to the specific blood composition and to the different biochemical characteristics and different adaptations within taxa. These findings highlight the importance of further research on the influence of biochemistry features on isotopic results, in this way a more accurate assessment will be possible for this factor, separating it from the dietary influences on stable isotopic values.
AB - Research using stable isotopes analysis (SIA) of carbon (δ13C) and nitrogen (δ15N) in blood components is lacking, because of the challenge of sample collection, processing, and storage in remote areas. There also is a paucity of information regarding the effect of tissue biochemical composition on isotopic ratios with few comparisons among taxa. We collected blood samples from shortfin mako sharks (n = 70; 2016) and Guadalupe fur seals (n = 25; 2017). All samples were centrifuged to obtain plasma from sharks and serum from the Guadalupe fur seals, and all the samples were prepared for SIA and analyzed using a Costech 4010 elemental analyzer interfaced with a Delta V Plus isotope ratio mass spectrometer. We found significant differences between plasma δ13C values of shortfin mako sharks (−17.6 ± 0.9‰) and serum of Guadalupe fur seals (−20.3 ± 1.2‰), but we did not find any differences for δ15N values between the two species. The differences in δ13C values between species are probably due to the specific blood composition and to the different biochemical characteristics and different adaptations within taxa. These findings highlight the importance of further research on the influence of biochemistry features on isotopic results, in this way a more accurate assessment will be possible for this factor, separating it from the dietary influences on stable isotopic values.
KW - Guadalupe fur seals
KW - biochemical features
KW - plasma
KW - serum
KW - shortfin mako sharks
KW - stable isotopes
UR - http://www.scopus.com/inward/record.url?scp=85074809209&partnerID=8YFLogxK
U2 - 10.1002/jez.2330
DO - 10.1002/jez.2330
M3 - Artículo
C2 - 31709773
AN - SCOPUS:85074809209
SN - 2471-5638
VL - 333
SP - 104
EP - 110
JO - Journal of Experimental Zoology Part A: Ecological and Integrative Physiology
JF - Journal of Experimental Zoology Part A: Ecological and Integrative Physiology
IS - 2
ER -