TY - JOUR
T1 - Isolation of Moderately Halophilic Bacteria in Saline Environments of Sonora State Searching for Proteolytic Hydrolases
AU - Corral, Juan Carlos Coronado
AU - De Los Santos Villalobos, Sergio
AU - Barrgàn, Lilia Arely Prado
AU - Figueroa, Juan Jose Buenrostro
AU - Vásquez-Murrieta, María Soledad
AU - Alvarado, María Isabel Estrada
AU - Chávez, Luis Alberto Cira
N1 - Publisher Copyright:
© 2018 2018 Luis Alberto Cira Chávez, published by Sciendo.
PY - 2018/1
Y1 - 2018/1
N2 - The aim of the study was to isolate moderately halophilic bacteria that produce proteolytic enzymes with industrial biotechnological value. Screening of halophiles from various saline habitats, led to the isolation of 210 moderately halophilic bacteria producing industrially important hydrolases; such as proteases, which are enzymes that hydrolyze the peptide bonds of proteins, represent one of the three groups of industrial enzymes. The present study of halophilic bacteria, producing proteases and isolated from different saline soils of Sonora State, was divided in two parts: the first part included screening of moderately halophiles bacteria collected from various regions, while the second part consisted of enzyme production by fermentation in liquid medium in flask. Three strains of bacteria with potential proteolytic activity (BLRMAM1066, BLCLAM1064, PPSE3AM1053) were selected to continue the electrophoresis and zymogram tests, obtaining molecular weights from 19 to 193 kDa.
AB - The aim of the study was to isolate moderately halophilic bacteria that produce proteolytic enzymes with industrial biotechnological value. Screening of halophiles from various saline habitats, led to the isolation of 210 moderately halophilic bacteria producing industrially important hydrolases; such as proteases, which are enzymes that hydrolyze the peptide bonds of proteins, represent one of the three groups of industrial enzymes. The present study of halophilic bacteria, producing proteases and isolated from different saline soils of Sonora State, was divided in two parts: the first part included screening of moderately halophiles bacteria collected from various regions, while the second part consisted of enzyme production by fermentation in liquid medium in flask. Three strains of bacteria with potential proteolytic activity (BLRMAM1066, BLCLAM1064, PPSE3AM1053) were selected to continue the electrophoresis and zymogram tests, obtaining molecular weights from 19 to 193 kDa.
KW - Isolation
KW - Moderate halophiles
KW - Proteases
KW - SDS-PAGE
KW - Saline areas
KW - Screening
UR - http://www.scopus.com/inward/record.url?scp=85051350231&partnerID=8YFLogxK
U2 - 10.1515/opag-2018-0021
DO - 10.1515/opag-2018-0021
M3 - Artículo
SN - 2391-9531
VL - 3
SP - 207
EP - 213
JO - Open Agriculture
JF - Open Agriculture
IS - 1
ER -