Aislamiento de una clona que contiene un gen de xilanasa a partir de una genoteca de Cellulomonas flavigena

Xylanases are catalytic enzymes with potential applications in the preparation of paper pulps, processing of agro-food raw materials and for the enzymatic conversion of hemicellulose to biomass. Xylanases are synthesized by a great diversity of genes stored in genomic libraries. In this work a genomic library of Cellulomonas flavigena was constructed in the bacteriophage lambda FIX II. Two primers were designed from an alignment of several aminoacid sequences of xylanases for the amplification of a 781 bp DNA fragment by PCR, which was used as a probe to screen the genomic library. One clone that carried a fragment of a xylanase gene was isolated.