Xylanases are catalytic enzymes with potential applications in the preparation of paper pulps, processing of agro-food raw materials and for the enzymatic conversion of hemicellulose to biomass. Xylanases are synthesized by a great diversity of genes stored in genomic libraries. In this work a genomic library of Cellulomonas flavigena was constructed in the bacteriophage lambda FIX II. Two primers were designed from an alignment of several aminoacid sequences of xylanases for the amplification of a 781 bp DNA fragment by PCR, which was used as a probe to screen the genomic library. One clone that carried a fragment of a xylanase gene was isolated.
|Original language||American English|
|Number of pages||5|
|Journal||Revista Mexicana de Ciencias Farmaceuticas|
|State||Published - 1 Apr 2005|
Mayorga Reyes, L., Gutiérrez N, A., Salgado, L. M., & Ponce N, T. (2005). Isolation of a clone containing a xylanase gene from a genomic library of Cellulomonas flavigena. Revista Mexicana de Ciencias Farmaceuticas, 5-9. https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=20444477802&origin=inward