Isolation of a clone containing a xylanase gene from a genomic library of Cellulomonas flavigena

Lino Mayorga Reyes, Angélica Gutiérrez N, Luis M. Salgado, Teresa Ponce N

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Abstract

Xylanases are catalytic enzymes with potential applications in the preparation of paper pulps, processing of agro-food raw materials and for the enzymatic conversion of hemicellulose to biomass. Xylanases are synthesized by a great diversity of genes stored in genomic libraries. In this work a genomic library of Cellulomonas flavigena was constructed in the bacteriophage lambda FIX II. Two primers were designed from an alignment of several aminoacid sequences of xylanases for the amplification of a 781 bp DNA fragment by PCR, which was used as a probe to screen the genomic library. One clone that carried a fragment of a xylanase gene was isolated.
Original languageAmerican English
Pages (from-to)5-9
Number of pages5
JournalRevista Mexicana de Ciencias Farmaceuticas
StatePublished - 1 Apr 2005
Externally publishedYes

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