TY - JOUR
T1 - Isolation and characterization of exosomes released from mosquito cells infected with dengue virus
AU - Reyes-Ruiz, José Manuel
AU - Osuna-Ramos, Juan Fidel
AU - De Jesús-González, Luis Adrián
AU - Hurtado-Monzón, Arianna Mahely
AU - Farfan-Morales, Carlos Noe
AU - Cervantes-Salazar, Margot
AU - Bolaños, Jeni
AU - Cigarroa-Mayorga, Oscar E.
AU - Martín-Martínez, Eduardo San
AU - Medina, Fernando
AU - Fragoso-Soriano, Rogelio Jaime
AU - Chávez-Munguía, Bibiana
AU - Salas-Benito, Juan Santiago
AU - del Angel, Rosa M.
N1 - Publisher Copyright:
© 2019 Elsevier B.V.
PY - 2019/6
Y1 - 2019/6
N2 - Exosomes are endocytic origin small-membrane vesicles secreted to the extracellular space by most cell types. Exosomes released from virus infected-cells can mediate the cell-to-cell communication to promote or modulate viral transmission. Dengue virus (DENV) is an arbovirus transmitted by Aedes mosquitoes bite to humans. Interestingly, the role of exosomes during the DENV infection in mammalian cells has already been described. However, little is known about exosomes derived from infected mosquito cells. Thus, the exosomes released from DENV-infected C6/36 cells were isolated, purified and analyzed using an antibody against the tetraspanin CD9 from human that showed cross-reactivity with the homologs to human CD9 found in Aedes albopictus (AalCD9). The exosomes from DENV infected cells were larger than the exosomes secreted from uninfected cells, contained virus-like particles, and they were able to infect naïve C6/36 cells, suggesting that exosomes are playing a role in virus dissemination.
AB - Exosomes are endocytic origin small-membrane vesicles secreted to the extracellular space by most cell types. Exosomes released from virus infected-cells can mediate the cell-to-cell communication to promote or modulate viral transmission. Dengue virus (DENV) is an arbovirus transmitted by Aedes mosquitoes bite to humans. Interestingly, the role of exosomes during the DENV infection in mammalian cells has already been described. However, little is known about exosomes derived from infected mosquito cells. Thus, the exosomes released from DENV-infected C6/36 cells were isolated, purified and analyzed using an antibody against the tetraspanin CD9 from human that showed cross-reactivity with the homologs to human CD9 found in Aedes albopictus (AalCD9). The exosomes from DENV infected cells were larger than the exosomes secreted from uninfected cells, contained virus-like particles, and they were able to infect naïve C6/36 cells, suggesting that exosomes are playing a role in virus dissemination.
KW - C6/36 cells
KW - Dengue virus
KW - Exosomes
KW - Intraluminal vesicles (ILVs)
KW - Multivesicular bodies (MVBs)
UR - http://www.scopus.com/inward/record.url?scp=85063941388&partnerID=8YFLogxK
U2 - 10.1016/j.virusres.2019.03.015
DO - 10.1016/j.virusres.2019.03.015
M3 - Artículo
C2 - 30930201
SN - 0168-1702
VL - 266
SP - 1
EP - 14
JO - Virus Research
JF - Virus Research
ER -
